| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 322,687 | A→G | I91T (ATC→ACC) | betA ← | choline dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 322,687 | 0 | A | G | 94.4% | 50.4 / ‑3.1 | 18 | I91T (ATC→ACC) | betA | choline dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (7/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01 | |||||||||||
TCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAT > NZ_CP009273/322600‑322768 | tCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTc < 1:44293/90‑1 (MQ=255) gAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAg > 1:302534/1‑90 (MQ=255) tCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATc < 1:266945/90‑1 (MQ=255) ttCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCAccc < 2:131175/90‑1 (MQ=255) ggTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATc < 1:30577/73‑1 (MQ=255) ggTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATc > 2:30577/1‑73 (MQ=255) aTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt < 1:151971/90‑1 (MQ=255) aTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt < 1:189463/90‑1 (MQ=255) tCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACcgc < 1:269923/67‑1 (MQ=255) tCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACcgc > 2:269923/1‑67 (MQ=255) cATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGGTATTCATaa > 1:34668/1‑90 (MQ=255) aTGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACg > 1:103994/1‑73 (MQ=255) aTGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACg < 2:103994/73‑1 (MQ=255) tGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATgcggc > 1:132460/1‑57 (MQ=255) tGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATgcggc < 2:132460/57‑1 (MQ=255) tGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACg < 1:334722/72‑1 (MQ=255) tGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACg > 2:334722/1‑72 (MQ=255) tGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt < 2:26118/90‑1 (MQ=255) | TCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAT > NZ_CP009273/322600‑322768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |