Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,888,711	A→G	V143A (GTC→GCC)	ygcQ ←	electron transfer flavoprotein subunit alpha/FixB family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,888,711	0	A	G	100.0%	16.9 / NA	7	V143A (GTC→GCC)	ygcQ	electron transfer flavoprotein subunit alpha/FixB family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/4);  total (3/4)

GCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGG  >  NZ_CP009273/2888625‑2888794                                                                                       |                                                                                    gCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGGCaa                                                                                  >  1:21105/1‑90 (MQ=255) gCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGGCaa                                                                                  <  1:249181/90‑1 (MQ=255)                     aaTGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGGCAATGTTCAGTTGTTGCATTCCa                                                              >  2:112066/1‑90 (MQ=255)                                                 ccTTTGTTCGAACCAGCCAGTCCGGAAACGCACCCGGGGCAATGTTCAGTTGATGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTc                                  <  1:127073/90‑1 (MQ=255)                                                                    gTCCGGAAGCGCCCCCGGGGCAATGTTCAGTTGTTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGa               <  2:207172/90‑1 (MQ=255)                                                                              gcACCCGGGGCAATGTTCAGTTGTTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGc     >  1:305107/1‑90 (MQ=255)                                                                                 cccGGGGCAATGTTCAGTTGTTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCggg  <  1:163003/90‑1 (MQ=255)                                                                                       |                                                                                    GCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGG  >  NZ_CP009273/2888625‑2888794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑