Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,369,157	T→C	G310G (GGT→GGC)	dcuC →	anaerobic C4‑dicarboxylate transporter DcuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,369,157	0	T	C	100.0%	46.9 / NA	16	G310G (GGT→GGC)	dcuC	anaerobic C4‑dicarboxylate transporter DcuC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/4);  total (12/4)

GTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGG  >  NZ_CP009273/3369091‑3369242                                                                   |                                                                                      gTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAAc                                                                >  2:29647/1‑90 (MQ=255)         gCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCtggtgg                                                        >  2:183523/1‑90 (MQ=255)              cATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTcgcg                                                   >  2:14477/1‑90 (MQ=255)              cATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTcgcg                                                   >  2:224185/1‑90 (MQ=255)                                     cGATGTGCAGGCGTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCtt                            >  1:93456/1‑90 (MQ=255)                                     cGATGTGCAGGCGTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCtt                            >  2:284366/1‑90 (MQ=255)                                       aTGTGCAGGCGTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTaa                          >  1:9537/1‑90 (MQ=255)                                       aTGTGCAGGCGTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTaa                          >  2:180363/1‑90 (MQ=255)                                       aTGTGCAGGCGTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTaa                          >  2:198357/1‑90 (MQ=255)                                            cAGGCGTTTTATGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAacgacg                     >  1:312124/1‑90 (MQ=255)                                                 gTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTgg                >  1:299727/1‑90 (MQ=255)                                                 gTTTTTTGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTgg                >  2:306336/1‑90 (MQ=255)                                                     tttGACGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACt            <  1:148876/90‑1 (MQ=255)                                                          cGGCATGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGa       <  2:221263/90‑1 (MQ=255)                                                            gCATGGGCACGCAGTTTGCCACCGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGCCGATTGGCACTGTCGATg     <  1:284344/90‑1 (MQ=255)                                                               tGGGCACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCgg  <  2:247307/90‑1 (MQ=255)                                                                   |                                                                                      GTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTCGCGGGTGAAATATTTGCGAAAGGCTTAACGACGATTGGCACTGTCGATGCGG  >  NZ_CP009273/3369091‑3369242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑