Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,840,691	T→G	N432H (AAC→CAC)	uhpC ←	MFS transporter family glucose‑6‑phosphate receptor UhpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,840,691	0	T	G	100.0%	32.9 / NA	11	N432H (AAC→CAC)	uhpC	MFS transporter family glucose‑6‑phosphate receptor UhpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/7);  total (4/7)

AGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAG  >  NZ_CP009273/3840606‑3840775                                                                                      |                                                                                     aGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGT‑‑GATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCaaaaa                                                                                <  2:228905/90‑1 (MQ=255)       aaCCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGc                                                                            >  1:180208/1‑90 (MQ=255)        aCCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCa                                                                           <  1:134094/90‑1 (MQ=255)                                  gATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggcg                                                 <  1:242940/90‑1 (MQ=255)                                                        tGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACaaaaa                           >  2:112844/1‑90 (MQ=255)                                                           aTCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCgg                                                            <  1:117560/54‑1 (MQ=255)                                                           aTCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCgg                                                            >  2:117560/1‑54 (MQ=255)                                                                        cgGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCa           <  1:24374/90‑1 (MQ=255)                                                                           tgtCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggc                                                  <  1:219855/48‑1 (MQ=255)                                                                           tgtCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggc                                                  >  2:219855/1‑48 (MQ=255)                                                                                 ggCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAg  <  2:176308/90‑1 (MQ=255)                                                                                      |                                                                                     AGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAG  >  NZ_CP009273/3840606‑3840775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑