Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,057,873	C→G	W418C (TGG→TGC)	yihO ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,057,873	0	C	G	100.0%	36.6 / NA	12	W418C (TGG→TGC)	yihO	MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (5/7);  total (5/7)

GTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCA  >  NZ_CP009273/4057809‑4057952                                                                 |                                                                                gTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAAc                                                        <  1:18818/90‑1 (MQ=255) gTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAAc                                                        <  2:304016/90‑1 (MQ=255)           gAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGt                                              >  1:170335/1‑90 (MQ=255)                tgtAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCAt                                         <  1:80112/90‑1 (MQ=255)                       gAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTg                                  >  2:202928/1‑90 (MQ=255)                         aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg                                >  1:114840/1‑90 (MQ=255)                            ccATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGa                             <  1:77696/90‑1 (MQ=255)                                          gcaATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATaa               >  2:149343/1‑90 (MQ=255)                                               aatTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaa                                                          <  1:209573/42‑1 (MQ=255)                                               aatTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCaa                                                          >  2:209573/1‑42 (MQ=255)                                                      aGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTc   <  2:313390/90‑1 (MQ=255)                                                       gTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCa  <  1:4382/90‑1 (MQ=255)                                                                 |                                                                                GTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCA  >  NZ_CP009273/4057809‑4057952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑