| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,310,789 | C→T | A92A (GCG→GCA) | phnH ← | phosphonate C‑P lyase system protein PhnH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,310,789 | 0 | C | T | 93.8% | 48.6 / ‑3.8 | 16 | A92A (GCG→GCA) | phnH | phosphonate C‑P lyase system protein PhnH |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (5/10); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTGACGATATCGTTATTTAATGGGGTAGA > NZ_CP009273/4310703‑4310872 | gCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGcc < 1:318602/90‑1 (MQ=255) gcggTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAgc < 1:109996/90‑1 (MQ=255) tGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTgg > 2:108793/1‑90 (MQ=255) cGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAAc > 1:178979/1‑90 (MQ=255) ttGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGc < 2:178979/90‑1 (MQ=255) tGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCa < 2:135571/90‑1 (MQ=255) tGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGctc < 2:208146/90‑1 (MQ=255) gTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGCTATGAAAACCCAGGctct > 1:62473/1‑90 (MQ=255) tCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTgg < 1:98694/90‑1 (MQ=255) tCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTgg > 2:53013/1‑90 (MQ=255) cTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTg > 1:131161/1‑90 (MQ=255) ccTCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTGACGATATCGt < 2:59828/90‑1 (MQ=255) tCATCCGTCACCGCGAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTGACGATATCGTTa < 1:438/90‑1 (MQ=255) gcgAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTGACGATATCGTTATTTAATGGGGTa > 2:192688/1‑90 (MQ=255) cgAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTGACGATATCGTTATTTAATGGGGTAg < 2:151047/90‑1 (MQ=255) gAAGGTTGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTGACGATATCGTTATTTAATGGGGTAGa < 1:150593/90‑1 (MQ=255) | GCACCCGCTTCCGGCGCAACGGCGGTGCCGGTGGAAAGGGCGTTGAGCTGTTCGCTGGAAATCGCCTCATCCGTCACCGCGAAGGTCGCCTGTTCCGGCTGGCTGACCAGCGGCGCGTTGGTATGAAAACGCAGGCTCTGGTTGACGATATCGTTATTTAATGGGGTAGA > NZ_CP009273/4310703‑4310872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |