Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	601,986	A→G	F285S (TTC→TCC)	ybdK ←	YbdK family carboxylate‑amine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	601,986	0	A	G	100.0%	43.7 / NA	14	F285S (TTC→TCC)	ybdK	YbdK family carboxylate‑amine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/5);  total (9/5)

TTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCC  >  NZ_CP009273/601918‑602066                                                                     |                                                                                 ttAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATAcagca                                                             >  1:287914/1‑90 (MQ=255)                 tcCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTg                                             <  2:287914/90‑1 (MQ=255)                       gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                       >  2:344931/1‑90 (MQ=255)                            gATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACg                                  >  2:87574/1‑90 (MQ=255)                               cGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCCGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGc                               >  1:243232/1‑90 (MQ=255)                                    atgaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTcc                          <  1:306308/90‑1 (MQ=255)                                     tgaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCg                         <  1:344931/90‑1 (MQ=255)                                      gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGt                        >  1:301337/1‑90 (MQ=255)                                      gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGt                        >  2:226076/1‑90 (MQ=255)                                           cTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa                   >  1:286056/1‑90 (MQ=255)                                              cAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAAcc                <  2:151916/90‑1 (MQ=255)                                                     aTAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGc         >  1:106809/1‑90 (MQ=255)                                                          gACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAg    >  2:290379/1‑90 (MQ=255)                                                            cAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGcc  <  2:45487/90‑1 (MQ=255)                                                                     |                                                                                 TTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCC  >  NZ_CP009273/601918‑602066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑