Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	609,731	A→G	D40G (GAT→GGT)	entF →	enterobactin non‑ribosomal peptide synthetase EntF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	609,731	0	A	G	87.5%	17.7 / ‑4.9	8	D40G (GAT→GGT)	entF	enterobactin non‑ribosomal peptide synthetase EntF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (3/4);  minor base T (0/1);  total (3/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAA  >  NZ_CP009273/609648‑609809                                                                                    |                                                                               cGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCGGCCTGGAGCGTTGCGCATTACGTTGAGTTAACCGGAAAGGTTGTTTCGcc                                                                          <  1:286750/90‑1 (MQ=255) cGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGcc                                                                          >  1:340161/1‑90 (MQ=255)        tGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTg                                                                   >  2:233560/1‑90 (MQ=255)                                        cGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGc                                   <  1:233560/90‑1 (MQ=255)                                                        cATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGACACGCTGCGGATg                   <  2:214930/90‑1 (MQ=255)                                                                tGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTAc           <  2:348418/90‑1 (MQ=255)                                                                      aaCCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGa     >  1:294635/1‑90 (MQ=255)                                                                         cGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATaa  <  1:50499/90‑1 (MQ=255)                                                                                    |                                                                               CGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAA  >  NZ_CP009273/609648‑609809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑