Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,167,328	T→C	E698G (GAA→GGA)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,167,328	0	T	C	100.0%	18.5 / NA	7	E698G (GAA→GGA)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/1);  total (6/1)

AGCAGGCCTAAATCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGC  >  NZ_CP009273/1167244‑1167401                                                                                     |                                                                          agcagGCCTAAATCTTTAAACTTGACGTCCCTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCa                                                                      <  2:285209/90‑1 (MQ=255)     ggCCTAAATCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGa                                                                  >  2:81905/1‑90 (MQ=255)                                           ttGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATc                            >  1:265295/1‑90 (MQ=255)                                           ttGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATc                            >  2:330937/1‑90 (MQ=255)                                                  tACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGa                     >  1:320718/1‑90 (MQ=255)                                                                     tttCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGc  >  1:322729/1‑90 (MQ=255)                                                                     tttCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATCTCGATACGTACCGGCCAGTTGGc  >  1:132988/1‑90 (MQ=255)                                                                                     |                                                                          AGCAGGCCTAAATCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGC  >  NZ_CP009273/1167244‑1167401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑