| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,431,536 | T→C | E1169G (GAA→GGA) | nifJ ← | pyruvate:ferredoxin (flavodoxin) oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,431,536 | 0 | T | C | 100.0% | 11.3 / NA | 5 | E1169G (GAA→GGA) | nifJ | pyruvate:ferredoxin (flavodoxin) oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (4/1); total (4/1) | |||||||||||
CTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTT > NZ_CP009273/1431479‑1431600 | cTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCa > 2:77553/1‑90 (MQ=255) tATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTgcagc > 1:158654/1‑90 (MQ=255) gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt > 2:147198/1‑90 (MQ=255) tttAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATc < 1:128599/82‑1 (MQ=255) tttAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATc > 2:128599/1‑82 (MQ=255) | CTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTT > NZ_CP009273/1431479‑1431600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |