| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,507,468 | C→A | P132Q (CCG→CAG) | ydcT → | ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,507,468 | 0 | C | A | 83.3% | 23.0 / 1.3 | 12 | P132Q (CCG→CAG) | ydcT | ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (5/5); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
AAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGA > NZ_CP009273/1507384‑1507549 | aaGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACAGTcac < 1:210214/90‑1 (MQ=255) agcGGCACGCAATGGCGCAATAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACAGTCACAACTTTCTGGTGGTCAgc < 1:244177/90‑1 (MQ=255) gcGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACAGTCACAACTTTCTGGTGGTCAgcg > 1:252506/1‑90 (MQ=255) gcgcAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACAGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCt > 2:91250/1‑90 (MQ=255) gcAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACAGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTAt > 1:84354/1‑90 (MQ=255) cAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATc > 2:179399/1‑90 (MQ=255) tGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACAGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCAt < 2:212476/90‑1 (MQ=255) gTGGCGTTGGGGTTTGTACATCAACGTAAACAGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGtgaat < 2:151349/90‑1 (MQ=255) ttGGGGTTTGTACATCAACGTAAACAGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACcg < 1:301554/90‑1 (MQ=255) ttGTACATCAACGTAAACAGTCACAACTTTCTGGTGGTCAGCGTCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTAt > 1:18647/1‑90 (MQ=255) cATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTg < 1:179399/90‑1 (MQ=255) aCGTAAACAGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGa > 1:57433/1‑90 (MQ=255) | AAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGA > NZ_CP009273/1507384‑1507549 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |