Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	130,698	A→T	*866L (TAA→TTA)	acnB →	bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	130,698	0	A	T	100.0%	22.4 / NA	7	*866L (TAA→TTA)	acnB	bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/1);  total (6/1)

CGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCA  >  NZ_CP009273/130625‑130760                                                                          |                                                               cGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCAcgc                                                <  1:215407/90‑1 (MQ=255)              tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAAc                                   >  1:97597/1‑90 (MQ=255)                     gCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACaa                            >  1:143002/1‑90 (MQ=255)                                   ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga              >  2:328491/1‑90 (MQ=255)                                      agaAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAt           >  1:56219/1‑90 (MQ=255)                                        aaaGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc         >  1:229536/1‑90 (MQ=255)                                               aTGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCa  >  1:133424/1‑90 (MQ=255)                                                                          |                                                               CGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCA  >  NZ_CP009273/130625‑130760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑