Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,568,198	A→G	F417S (TTC→TCC)	pqqL ←	insulinase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,568,198	0	A	G	100.0%	51.8 / NA	16	F417S (TTC→TCC)	pqqL	insulinase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/9);  total (7/9)

AACTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATC  >  NZ_CP009273/1568111‑1568253                                                                                        |                                                        aaCTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGAc                                                       <  1:309623/90‑1 (MQ=255) aaCTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGAc                                                       <  2:1358/90‑1 (MQ=255)   ctgctgCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACgg                                                     <  1:189926/90‑1 (MQ=255)   ctgctgCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACgg                                                     <  2:194755/90‑1 (MQ=255)       tgcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGta                                                 >  1:59280/1‑90 (MQ=255)       tgcCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGta                                                 <  2:157476/90‑1 (MQ=255)                         cTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGAt                               <  2:102849/90‑1 (MQ=255)                                 ggTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCg                       >  1:326044/1‑90 (MQ=255)                                 ggTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCg                       >  1:38444/1‑90 (MQ=255)                                  gTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGa                      >  1:110223/1‑90 (MQ=255)                                          ctgcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACgg                                                     <  1:127772/51‑1 (MQ=255)                                          ctgcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACgg                                                     >  2:127772/1‑51 (MQ=255)                                            gcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCa            >  1:174875/1‑90 (MQ=255)                                            gcCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCa            >  2:179167/1‑90 (MQ=255)                                                     gTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAAt   <  2:174875/90‑1 (MQ=255)                                                      tttCGAAAGTTGATATGTCTCTTCCGGCGACAAGGACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATc  <  1:165649/90‑1 (MQ=255)                                                                                        |                                                        AACTGCTGCCATTTTTCCGCCAGACTTTGCACGGTAATTTGCTGCCACAGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATC  >  NZ_CP009273/1568111‑1568253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑