Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,579,109	A→C	G545G (GGT→GGG)	ydeP ←	acid resistance putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,579,109	0	A	C	100.0%	26.0 / NA	9	G545G (GGT→GGG)	ydeP	acid resistance putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/2);  total (7/2)

CAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGCA  >  NZ_CP009273/1579025‑1579194                                                                                     |                                                                                      caGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc                                                                                  >  2:134138/1‑90 (MQ=255)   gATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggcgg                                                                                >  1:18594/1‑90 (MQ=255)                           ggggTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCAt                                                        >  1:342342/1‑90 (MQ=255)                                aGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatc                                                   <  1:35867/90‑1 (MQ=255)                                 gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca                                                  >  2:328533/1‑90 (MQ=255)                                   gctgcCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatcatc                                                <  1:231807/90‑1 (MQ=255)                                      gcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGAc                                             >  1:124976/1‑90 (MQ=255)                                      gcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGAc                                             >  2:257264/1‑90 (MQ=255)                                                                                 ttACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGCa  >  2:342483/1‑90 (MQ=255)                                                                                     |                                                                                      CAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGCA  >  NZ_CP009273/1579025‑1579194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑