Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,662,649	T→G	H58P (CAC→CCC)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,662,649	0	T	G	100.0%	17.9 / NA	7	H58P (CAC→CCC)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/2);  total (5/2)

GCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCG  >  NZ_CP009273/1662566‑1662727                                                                                    |                                                                               gCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCtt                                                                          >  2:288480/1‑90 (MQ=255)           aGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCAtctc                                                                >  1:347422/1‑90 (MQ=255)                     accaGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAAtt                                                      <  2:16965/90‑1 (MQ=255)                             cACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGAt                                              >  2:274357/1‑90 (MQ=255)                                                         tcttTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACgg                  >  1:108813/1‑90 (MQ=255)                                                                tttCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGAt           >  2:113991/1‑90 (MQ=255)                                                                         ctTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATAcgcg  <  1:113991/90‑1 (MQ=255)                                                                                    |                                                                               GCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCG  >  NZ_CP009273/1662566‑1662727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑