Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,673,542	A→G	K287E (AAG→GAG)	ydgH →	DUF1471 family protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,673,542	0	A	G	100.0%	30.6 / NA	13	K287E (AAG→GAG)	ydgH	DUF1471 family protein YdgH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/7);  total (6/7)

GTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATA  >  NZ_CP009273/1673466‑1673627                                                                             |                                                                                      gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                          <  1:14848/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                                          >  2:133805/1‑90 (MQ=255)      aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt                                                                     >  1:243946/1‑90 (MQ=255)                   ctctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGCGCTAAGTATTACCACATCACCCg                                                        >  1:137884/1‑90 (MQ=255)                     ctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGGGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGcc                                                      >  2:212049/1‑90 (MQ=255)                            cTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCa                                               <  2:243946/90‑1 (MQ=255)                                   aaCATGACCAAAGCCCCCTATCAGGTTGCAAACGGTGCGGGGGAAAAGGGTGCAAATTATTACCACATCCCCCGCCAGTGGCAGGAACGt                                        <  1:296935/90‑1 (MQ=255)                                                     tATCAGGTTGCAAAACGGGCCGCGGAAAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc                      <  2:6425/90‑1 (MQ=255)                                                           gTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTAc                                                                   >  1:150069/1‑39 (MQ=39)                                                           gTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTAc                                                                   <  2:150069/39‑1 (MQ=255)                                                                    cGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGGAATAACCTGACCGTCAGCGCAGATCTGtat       >  1:315609/1‑90 (MQ=255)                                                                        ccGCGGAAAAGGGTGTTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataaat   <  2:319487/90‑1 (MQ=255)                                                                         cgcgGAAAAGGGTGCTAAGTTTTACCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataaata  <  2:295052/90‑1 (MQ=255)                                                                             |                                                                                      GTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATA  >  NZ_CP009273/1673466‑1673627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑