Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,763,148	A→G	intergenic (‑206/‑183)	ydiJ ← / → ydiK	FAD‑binding and (Fe‑S)‑binding domain‑containing protein/AI‑2E family transporter YdiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,763,148	0	A	G	100.0%	35.2 / NA	12	intergenic (‑206/‑183)	ydiJ/ydiK	FAD‑binding and (Fe‑S)‑binding domain‑containing protein/AI‑2E family transporter YdiK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/3);  total (9/3)

TTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCC  >  NZ_CP009273/1763087‑1763232                                                              |                                                                                     ttACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGGCGAGTAAATCGTTTGCGTGTTGCCTGAg                                                          >  1:300695/1‑90 (MQ=255)           aaGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGGCGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTAcc                                                >  2:249944/1‑90 (MQ=255)           aaGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGGCGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTAcc                                                >  2:345989/1‑90 (MQ=255)                aCTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGGCGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACAtt                                           >  2:92273/1‑90 (MQ=255)                 cTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGGCGAGTAAATCGGTTGCGTGTTGCCTGAGTTGTTGTACAACAttt                                          >  1:232654/1‑90 (MQ=37)                       tGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGGCGAGTAAATCGTTTGCGGGTTGCCTGAGTTGTTGTACCACATTTTTTTCt                                    >  1:215503/1‑90 (MQ=255)                                  aGCCGCAGCACATGCAACTTGAAATACGGCGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCAt                         >  2:80648/1‑90 (MQ=255)                                           acaTGCAACTTGAAATACGGCGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATc                        <  1:190291/82‑1 (MQ=255)                                           acaTGCAACTTGAAATACGGCGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATc                        >  2:190291/1‑82 (MQ=255)                                               gCAACTTGAAATACGGCGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGc            <  2:300695/90‑1 (MQ=255)                                                   cTTGAAATACGGCGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGaa        >  2:325424/1‑90 (MQ=255)                                                         aTACGGCGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGcc  <  1:52647/90‑1 (MQ=255)                                                              |                                                                                     TTACAATAGTAAGTGACTGGGGTGAACGAACGTAGCCGCAGCACATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAATCGGCC  >  NZ_CP009273/1763087‑1763232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑