BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,108,443	C→A	noncoding (56/76 nt)	pheV →	tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,108,443	0	C	A	99.8%	2028.6 / NA	524	noncoding (56/76 nt)	pheV	tRNA‑Phe
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (253/269); total (254/270)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 540 total aligned reads displayed.

TTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCT  >  NC_000913/3108345‑3108540

ttgatagaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGa                                                                                                 <  2:3076112/101‑1 (MQ=255)

  nnntgaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGact                                                                                               <  2:11113264/98‑3 (MQ=255)

  gattgaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGAtt                                                                                               <  1:4125761/101‑1 (MQ=255)

  gattgaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGAtt                                                                                               <  1:3101967/101‑1 (MQ=255)

  gattgaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGAtt                                                                                               <  1:10924/101‑1 (MQ=255)

  gatagaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGGAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGAtt                                                                                               <  2:6289286/101‑1 (MQ=255)

   nnngacgagacgaGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGAtcc                                                                                              <  2:9625591/98‑3 (MQ=255)

    ttgaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTcc                                                                                             <  1:6709739/101‑1 (MQ=255)

     tgaCGAGACGAGGCGAATCAGGTTTAATGCGCCGCGTTGCCCGGATAGCACAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCg                                                                                            <  1:2982136/101‑1 (MQ=255)

      gacgagacgaGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGa                                                                                           <  1:2763153/101‑1 (MQ=255)

      gacgagacgaGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGa                                                                                           <  1:4969026/101‑1 (MQ=255)

       nnnagacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGCCCTTGGTTAGATTACGAg                                                                                          <  2:10433972/98‑1 (MQ=14)

       acgagacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAg                                                                                          <  1:13638066/101‑1 (MQ=255)

        cgagacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGt                                                                                         >  2:4362124/1‑101 (MQ=255)

         gagacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTc                                                                                        >  1:1961970/1‑101 (MQ=255)

         gagacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTc                                                                                        >  1:574664/1‑101 (MQ=255)

          agacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTcc                                                                                       >  1:14179060/1‑101 (MQ=255)

          agacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTcc                                                                                       <  1:10036467/101‑1 (MQ=255)

          agacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTcc                                                                                       <  1:1620782/101‑1 (MQ=255)

           gacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCg                                                                                      <  2:2883754/101‑1 (MQ=255)

           gacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCg                                                                                      >  2:7036568/1‑101 (MQ=255)

            atgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTcnnn                                                                                     >  2:11468818/3‑98 (MQ=255)

              gagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGc                                                                                   >  1:13216950/1‑101 (MQ=255)

              gagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGc                                                                                   >  1:4549202/1‑101 (MQ=255)

               agGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGca                                                                                  >  2:201068/1‑101 (MQ=255)

                   gAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGcaccac                                                                              >  1:3605720/1‑101 (MQ=38)

                    aaTCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGcaccnnn                                                                             >  2:7640552/1‑98 (MQ=255)

                     aTCAGGTTTAATGCGCCGCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTACTTGGTTAGATTCCGAGTCCGGGCACCCCTa                                                                            <  2:6797708/101‑1 (MQ=11)

                      tCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTaa                                                                           <  1:10424859/101‑1 (MQ=38)

                       ccggTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACtnnn                                                                          >  2:10825286/3‑98 (MQ=2)

                        nnntttAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAAtt                                                                         <  2:10176159/98‑1 (MQ=255)

                         ggTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTc                                                                        <  1:12642025/101‑1 (MQ=255)

                          gTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCt                                                                       >  1:2311921/1‑101 (MQ=255)

                          gTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCt                                                                       <  2:1848786/101‑1 (MQ=255)

                           nnnaaTGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCtt                                                                      <  2:11896115/98‑1 (MQ=255)

                             tAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGCCCGGGCACCACTAATTCTTaa                                                                    >  2:5632717/1‑101 (MQ=255)

                              aaTGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAg                                                                   >  1:10247557/1‑101 (MQ=255)

                                tGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCATAAGaa                                                                 <  1:14087066/101‑1 (MQ=255)

                                    ccccGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCg                                                             >  1:14661177/1‑101 (MQ=255)

                                      ccGTTGCCCGGATAGCTCAGTCGGTAGCACAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGcc                                                           <  1:3947913/101‑1 (MQ=255)

                                        gTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCca                                                         <  1:432132/101‑1 (MQ=255)

                                        gTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCca                                                         >  1:11448587/1‑101 (MQ=255)

                                        gTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCca                                                         <  1:9554541/101‑1 (MQ=255)

                                        gTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCca                                                         <  1:9277598/101‑1 (MQ=255)

                                        gTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCca                                                         >  1:6379105/1‑101 (MQ=255)

                                        gTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCca                                                         >  1:7730931/1‑101 (MQ=255)

                                        gTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCca                                                         <  1:4670710/101‑1 (MQ=255)

                                         ttGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCcac                                                        >  2:4802814/1‑101 (MQ=255)

                                          nnnccGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCcaca                                                       <  2:13921542/98‑1 (MQ=255)

                                           gCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACaa                                                      <  1:1331277/101‑1 (MQ=255)

                                            nnngtaTAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAg                                                     <  2:8674263/96‑1 (MQ=255)

                                            nnnggATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAg                                                     <  2:7302915/98‑1 (MQ=255)

                                            nnnggATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAg                                                     <  2:11234197/98‑1 (MQ=255)

                                               ggATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAgnnn                                                  >  2:10248202/1‑98 (MQ=255)

                                               ggATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAgnnn                                                  >  2:8863737/1‑98 (MQ=255)

                                                gATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCgg                                                 >  2:61327/1‑101 (MQ=255)

                                                   aGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGtt                                              >  1:6132976/1‑101 (MQ=255)

                                                    gCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACAACTAATTCTTAAGAACCCGCCCACAAGGCGGGttt                                             >  1:14502533/1‑101 (MQ=255)

                                                        aGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTgt                                         >  1:6993106/1‑100 (MQ=255)

                                                          nnngTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCtt                                       <  2:14179060/98‑1 (MQ=255)

                                                           nnntAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCttt                                      <  2:9004228/98‑1 (MQ=255)

                                                            ggTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAGCCTGCCCACAAGGCGGGTTTTTGCtttt                                     <  1:14275235/101‑1 (MQ=255)

                                                            ggTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCtttt                                     <  1:5539255/101‑1 (MQ=255)

                                                            ggTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCtttt                                     >  1:8400828/1‑101 (MQ=255)

                                                            ggTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCtnnn                                     >  2:14457602/1‑98 (MQ=255)

                                                             gTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTg                                    <  1:2486648/101‑1 (MQ=255)

                                                               agagCAGGGGATTGAAAATCCCTGTGTCCTTGGTTAGATTCCGAGCCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGa                                  <  1:8098081/101‑1 (MQ=255)

                                                                gagCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGAt                                 <  2:3986292/101‑1 (MQ=255)

                                                                  nnnggggATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCt                               <  2:9379470/98‑1 (MQ=255)

                                                                  gCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCt                               >  1:13967667/1‑101 (MQ=255)

                                                                   nnngggATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTg                              <  2:12133637/98‑1 (MQ=255)

                                                                   cAGGGGATTGAAAATCCCCGGGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTg                              >  2:3274609/1‑101 (MQ=255)

                                                                    aGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGa                             >  2:1059264/1‑101 (MQ=255)

                                                                      gggATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACa                           <  2:5093724/101‑1 (MQ=255)

                                                                         nnngAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATa                        <  2:13217472/98‑1 (MQ=255)

                                                                          tttaaaaTCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGACCCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATaa                       <  2:1599465/98‑1 (MQ=255)

                                                                          ttGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACaannn                       >  2:12578657/1‑98 (MQ=255)

                                                                          ttGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATaa                       <  1:11838314/101‑1 (MQ=255)

                                                                          nnnaaaaTCCCCGTGTTCTTGGTTAGATGCCGAGTCCGGGCACCAATAATTCTTAAGAACCCGCCCACAAGGCGGTTTTTTGCTTTTGGATCTGACAAtta                       <  2:14661177/98‑3 (MQ=37)

                                                                           tGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTTGATCTGACAAtnnn                      >  2:13464824/1‑98 (MQ=255)

                                                                             aaaaTCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCt                    <  1:8603079/101‑1 (MQ=255)

                                                                              aaaTCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCtt                   <  1:8984938/101‑1 (MQ=255)

                                                                              aaaTCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCtt                   <  1:2144465/101‑1 (MQ=255)

                                                                               nnnccccGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTc                  <  2:8400828/98‑1 (MQ=255)

                                                                                aTCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCa                 >  1:11271164/1‑101 (MQ=255)

                                                                                 tCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCGCAAGGTGGGTTTTTGCTTTTGGATCTGACAATAACCttnnn                >  2:13094036/1‑98 (MQ=38)

                                                                                 tCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCAc                >  2:6455816/1‑101 (MQ=255)

                                                                                 tCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCAc                <  2:3844523/101‑1 (MQ=255)

                                                                                 cccacGTGTCCTTGGTTAGATTACGAGTCCGGGCACCACTAATTCTTAAGACCCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCAc                <  2:5798889/97‑1 (MQ=38)

                                                                                  nnncGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACg               <  2:12387016/98‑1 (MQ=255)

                                                                                  ccccGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACg               <  2:353287/101‑1 (MQ=255)

                                                                                  ccccGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACg               <  2:4549202/101‑1 (MQ=255)

                                                                                    ccGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaa             <  1:7380845/101‑1 (MQ=255)

                                                                                    ccGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaa             <  1:1290947/101‑1 (MQ=255)

                                                                                     cGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaa            >  2:2564027/1‑101 (MQ=255)

                                                                                     cGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaa            >  2:80436/1‑101 (MQ=255)

                                                                                      gtgtCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACCATACCCTTCACGaaaa           <  1:3334732/101‑1 (MQ=255)

                                                                                         tCCTTTGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaaaaa        <  1:12373021/101‑1 (MQ=255)

                                                                                           cTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAAtt      >  1:12068026/1‑101 (MQ=255)

                                                                                            ttGGTTAGATTACGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaaaaannn     >  2:13805051/1‑98 (MQ=255)

TTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCT  >  NC_000913/3108345‑3108540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`