BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,410	0	C	T	53.1%	111.9 / inf	514	L185L (CTC→CTT)	yecD	isochorismatase family protein
Reads supporting (aligned to +/- strand): ref base C (122/119); new base T (163/110); total (285/229)
Fisher's exact test for biased strand distribution p-value = 4.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.31e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 538 total aligned reads displayed.

CGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCGCCACTTTAACT  >  NC_000913/1949311‑1949509

cGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTc                                                                                                     <  2:2454520/100‑1 (MQ=255)

  aaGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCaa                                                                                                   <  1:5520528/100‑1 (MQ=255)

  aaGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCaa                                                                                                   >  2:5494875/1‑100 (MQ=255)

  aaGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCaa                                                                                                   >  1:2722807/1‑100 (MQ=255)

      cGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCAcgcg                                                                                               >  1:4762971/1‑100 (MQ=255)

           gTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATg                                                                                          <  2:292975/100‑1 (MQ=255)

           gTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATg                                                                                          >  2:5962568/1‑100 (MQ=255)

            tAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGa                                                                                         >  2:1667995/1‑100 (MQ=255)

            tAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGa                                                                                         <  2:7053633/100‑1 (MQ=255)

             aGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAt                                                                                        >  1:3194383/1‑100 (MQ=255)

             aGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAt                                                                                        <  1:493280/100‑1 (MQ=255)

              gTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAtt                                                                                       <  1:3490310/100‑1 (MQ=255)

              gTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAtt                                                                                       <  2:5331717/100‑1 (MQ=255)

              gTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAtt                                                                                       >  2:1401449/1‑100 (MQ=255)

              gTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAtt                                                                                       >  1:4035630/1‑100 (MQ=255)

              gTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAtt                                                                                       <  1:6130243/100‑1 (MQ=255)

               tGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAttt                                                                                      >  1:5416631/1‑100 (MQ=255)

                   gccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACAt                                                                                  <  2:3048713/100‑1 (MQ=255)

                   gccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACAt                                                                                  <  1:2079946/100‑1 (MQ=255)

                     cAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCg                                                                                <  1:7124820/100‑1 (MQ=255)

                       gcgcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGt                                                                              >  1:1938091/1‑100 (MQ=255)

                        cgcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTc                                                                             <  2:1822198/100‑1 (MQ=255)

                        cgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTc                                                                             <  2:3984112/100‑1 (MQ=255)

                         gcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCt                                                                            <  1:5936873/100‑1 (MQ=255)

                            gAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTAcc                                                                         >  1:4684522/1‑100 (MQ=255)

                             agcaacACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCg                                                                        >  1:375865/1‑100 (MQ=255)

                                 gcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAAt                                                                    >  2:3491980/1‑100 (MQ=255)

                                 acacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAAt                                                                    <  1:2252685/99‑1 (MQ=255)

                                   acaATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATgg                                                                  >  2:5706202/1‑100 (MQ=255)

                                    caATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGt                                                                 <  2:6359708/100‑1 (MQ=255)

                                    caATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGt                                                                 <  1:3385509/100‑1 (MQ=255)

                                     aaTAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTc                                                                <  2:5635329/100‑1 (MQ=255)

                                     aaTAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTc                                                                >  1:5680931/1‑100 (MQ=255)

                                     aaTAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTc                                                                >  2:2597653/1‑100 (MQ=255)

                                        aaCAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCa                                                             <  2:1951008/100‑1 (MQ=255)

                                         aCAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCAt                                                            >  2:5089699/1‑100 (MQ=255)

                                          cAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATc                                                           >  1:1449983/1‑100 (MQ=255)

                                           aGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATcc                                                          <  2:2190124/100‑1 (MQ=255)

                                            gTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCt                                                         <  2:3678756/100‑1 (MQ=255)

                                            gTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCt                                                         <  1:1576505/100‑1 (MQ=255)

                                             tATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTa                                                        >  2:2328728/1‑100 (MQ=255)

                                              aTCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTaa                                                       >  1:5101436/1‑100 (MQ=255)

                                               ttaatcATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTaaa                                                      >  1:4259374/6‑100 (MQ=255)

                                                taatcATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAAt                                                     >  1:6211138/5‑100 (MQ=255)

                                                cAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAAt                                                     >  2:426489/1‑100 (MQ=255)

                                                    cATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGt                                                 <  2:5189718/100‑1 (MQ=255)

                                                     atatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTg                                                >  1:3281856/1‑100 (MQ=255)

                                                     atatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTg                                                >  1:2828012/1‑100 (MQ=255)

                                                     atatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTg                                                >  2:156369/1‑100 (MQ=255)

                                                     atatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTg                                                <  2:3745787/100‑1 (MQ=255)

                                                      tatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGGTCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGc                                               >  2:4321447/1‑100 (MQ=255)

                                                      tatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGc                                               >  2:6593486/1‑100 (MQ=255)

                                                      tatCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGc                                               <  1:2909681/100‑1 (MQ=255)

                                                        tCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCgg                                             >  2:6182394/1‑100 (MQ=255)

                                                         cTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGt                                            <  1:4979859/100‑1 (MQ=255)

                                                         cTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGt                                            <  1:6927673/100‑1 (MQ=255)

                                                          tACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGtt                                           <  2:2685256/100‑1 (MQ=255)

                                                           aCCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTg                                          >  1:5846827/1‑100 (MQ=255)

                                                           aCCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTg                                          <  1:3846095/100‑1 (MQ=255)

                                                            cccGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTgg                                         >  2:796633/1‑100 (MQ=255)

                                                             ccGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTggg                                        <  2:2292064/100‑1 (MQ=255)

                                                                cgcATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGAt                                     <  1:99339/100‑1 (MQ=255)

                                                                cgcATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGAt                                     <  1:6651408/100‑1 (MQ=255)

                                                                   aTCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCAc                                  <  2:4572423/100‑1 (MQ=255)

                                                                   aTCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCAc                                  <  1:5179531/100‑1 (MQ=255)

                                                                    tCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCAcc                                 <  1:6354049/100‑1 (MQ=255)

                                                                    tCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCAcc                                 >  2:6668059/1‑100 (MQ=255)

                                                                         cGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCt                            >  2:1254335/1‑100 (MQ=255)

                                                                          gtgtGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCtt                           <  1:2377051/100‑1 (MQ=255)

                                                                           tgtgCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTg                          >  2:3338191/1‑100 (MQ=255)

                                                                           tgtgCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTg                          >  1:6450575/1‑100 (MQ=255)

                                                                            gtgCCTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGa                         <  1:5252788/100‑1 (MQ=255)

                                                                             tgCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGaa                        <  1:132886/100‑1 (MQ=255)

                                                                             tgCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGaa                        <  2:2868951/100‑1 (MQ=255)

                                                                             tgCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGaa                        <  1:5021657/100‑1 (MQ=255)

                                                                             tgCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGaa                        >  1:2492922/1‑100 (MQ=255)

                                                                             tgCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGaa                        >  2:5817118/1‑100 (MQ=255)

                                                                             tgCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGaa                        <  1:1620150/100‑1 (MQ=255)

                                                                                gTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAagag                     >  2:4935956/1‑100 (MQ=255)

                                                                                gTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAagag                     <  1:4570534/100‑1 (MQ=255)

                                                                                 tAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGt                    >  1:3064188/1‑100 (MQ=255)

                                                                                   gCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTAt                  >  2:6486543/1‑100 (MQ=255)

                                                                                      tGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGcc               >  1:631252/1‑100 (MQ=255)

                                                                                      tGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGcc               <  1:4402349/100‑1 (MQ=255)

                                                                                      tGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGcc               <  1:4176798/100‑1 (MQ=255)

                                                                                       ggAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGccc              >  2:882794/1‑100 (MQ=255)

                                                                                        gAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGCCCg             >  2:5422851/1‑100 (MQ=255)

                                                                                        gAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGCCCg             >  1:2142059/1‑100 (MQ=255)

                                                                                        gAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGCCCg             >  1:1036408/1‑100 (MQ=255)

                                                                                        gAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCg             >  1:3018319/1‑100 (MQ=255)

                                                                                         aaGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGCCCGc            >  1:6780339/1‑100 (MQ=255)

                                                                                          agagATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGCCCGcc           >  2:928562/1‑100 (MQ=255)

                                                                                          agagATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGCCCGcc           >  2:2059865/1‑100 (MQ=255)

                                                                                             gaTCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGCCCGCCACt        <  2:6852838/100‑1 (MQ=255)

                                                                                             gaTCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGCCCGCCACt        >  1:6041402/1‑100 (MQ=255)

                                                                                             gaTCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGTATCACCAGCCTTGAAGAGTATGCCCGCCACt        >  1:1693823/1‑100 (MQ=255)

                                                                                                ccTCAACGGTTTATGATTTACATCGGTCTCCCGAAATGGTCCCACCCAAAATGGGTGGGGTGGGGGATCCCCAGCCTTAAAGAGTATGCCCGCCACTTTa     <  2:25397/100‑1 (MQ=255)

                                                                                                 cTCACCGGGTTATGTTTTACATCGGTCTACCGCAATGGTCGCATCCTAAAGGGGTGGGGTGGGGGATCCCCAGCCTTGAAGAGTATGCCCGCCACTTTaa    <  2:469905/100‑1 (MQ=255)

                                                                                                 cTCACCGCGTTATGTTTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGACCCCCAGCCTTGAAGATTATGCCCGCCACTTTaa    <  2:6394002/100‑1 (MQ=255)

                                                                                                 cTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCGCCACTTTaa    >  1:1257262/1‑100 (MQ=255)

CGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGTGCGGTTGGGGATCACCAGCCTTGAAGAGTATGCCCGCCACTTTAACT  >  NC_000913/1949311‑1949509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`