Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,162,146 C→G A162G (GCA→GGA)  btuB → vitamin B12/cobalamin outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,162,1460CG99.7% 1230.3 / NA 284A162G (GCA→GGA) btuBvitamin B12/cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (144/139);  minor base T (0/1);  total (144/140)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

TCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCA  >  NC_000913/4162050‑4162242
                                                                                                |                                                                                                
tCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCaggagg                                                                                               >  1:4912521/1‑100 (MQ=255)
tCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCaggagg                                                                                               <  2:3260610/100‑1 (MQ=255)
  cGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGt                                                                                             <  2:3959899/100‑1 (MQ=255)
   gTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTg                                                                                            <  1:4164963/100‑1 (MQ=255)
   gTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTg                                                                                            >  2:3531832/1‑100 (MQ=255)
    tGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTgg                                                                                           <  2:4143011/100‑1 (MQ=255)
     gggCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTggg                                                                                          <  2:4902643/100‑1 (MQ=255)
     gggCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTggg                                                                                          <  2:2687728/100‑1 (MQ=255)
     gggCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATAATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTggg                                                                                          <  2:1399907/100‑1 (MQ=255)
      ggCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTgggg                                                                                         >  2:2877795/1‑100 (MQ=255)
       gCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGa                                                                                        >  2:2052469/1‑100 (MQ=255)
       gCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGa                                                                                        <  1:6512716/100‑1 (MQ=255)
        gcgctCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGaa                                                                                       <  2:3447930/99‑1 (MQ=255)
         cgcgCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAg                                                                                      <  2:2545581/100‑1 (MQ=255)
            gcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCaa                                                                                   <  1:6710555/100‑1 (MQ=255)
             cTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAAt                                                                                  <  2:3406023/100‑1 (MQ=255)
              tCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATa                                                                                 >  2:308736/1‑100 (MQ=255)
                  cTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTa                                                                             <  1:1017141/100‑1 (MQ=255)
                  cTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTa                                                                             >  2:13732/1‑100 (MQ=255)
                  cTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTa                                                                             <  1:4837080/100‑1 (MQ=255)
                  cTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTa                                                                             <  2:6445981/100‑1 (MQ=255)
                     tttATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCa                                                                          >  1:990743/1‑100 (MQ=255)
                     tttATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCa                                                                          >  2:6118533/1‑100 (MQ=255)
                      ttATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAg                                                                         <  2:2231404/100‑1 (MQ=255)
                      ttATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAg                                                                         >  1:6444541/1‑100 (MQ=255)
                       tATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGa                                                                        >  1:1235808/1‑100 (MQ=255)
                       tATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGa                                                                        >  1:1388117/1‑100 (MQ=255)
                        aTGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGCCGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGaa                                                                       <  2:3989441/100‑1 (MQ=255)
                        aTGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGaa                                                                       >  2:6813741/1‑100 (MQ=255)
                           gTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTa                                                                    <  2:6359692/100‑1 (MQ=255)
                           gTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTa                                                                    <  2:1130489/100‑1 (MQ=255)
                           gTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTa                                                                    <  1:235312/100‑1 (MQ=255)
                            ttCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTat                                                                   <  1:6149428/100‑1 (MQ=255)
                            ttCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTat                                                                   <  2:3835465/100‑1 (MQ=255)
                               cGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTatgat                                                                >  2:5571505/1‑100 (MQ=255)
                                 aTGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGt                                                              >  1:947432/1‑100 (MQ=255)
                                  tGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGtc                                                             >  2:1850230/1‑100 (MQ=255)
                                    cAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGtctc                                                           <  1:1773987/100‑1 (MQ=255)
                                     aaTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTctct                                                          >  1:6048024/1‑100 (MQ=255)
                                     aaTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTctct                                                          <  1:6345577/100‑1 (MQ=255)
                                     aaTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTctct                                                          >  2:5362146/1‑100 (MQ=255)
                                     aaTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTctct                                                          >  1:5035585/1‑100 (MQ=255)
                                      aTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTa                                                         >  1:4382517/1‑100 (MQ=255)
                                      aTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTa                                                         <  1:3629248/100‑1 (MQ=255)
                                      aTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTa                                                         >  2:7031624/1‑100 (MQ=255)
                                       tAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTAc                                                        <  1:2081640/100‑1 (MQ=255)
                                       tAGGCGGGGGGGGGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGAAAAAGTTATCAGAAATATGATGTCTCTAc                                                        >  2:1903999/1‑100 (MQ=255)
                                         ggCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgc                                                      >  2:6524545/1‑100 (MQ=255)
                                            ggggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgcagc                                                   >  1:7087224/1‑100 (MQ=255)
                                            ggggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgcagc                                                   <  2:5047696/100‑1 (MQ=255)
                                               gtggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaac                                                <  2:5520712/100‑1 (MQ=255)
                                               gtggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaac                                                <  2:2401381/100‑1 (MQ=255)
                                                tggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaaca                                               >  2:787772/1‑100 (MQ=255)
                                                 ggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaacaa                                              >  2:2177167/1‑100 (MQ=255)
                                                  gtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCaacaac                                             >  1:2965583/1‑100 (MQ=255)
                                                  gtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCaacaac                                             >  1:259180/1‑100 (MQ=255)
                                                      atatCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTggg                                         >  2:2987576/1‑100 (MQ=255)
                                                       tatCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTgggg                                        >  1:3791788/1‑100 (MQ=255)
                                                       tatCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTgggg                                        >  2:4323774/1‑100 (MQ=255)
                                                         tcatcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGa                                      <  2:2623785/100‑1 (MQ=255)
                                                         tcatcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGa                                      >  1:420095/1‑100 (MQ=255)
                                                         tcatcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGa                                      <  1:74258/100‑1 (MQ=255)
                                                          catcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGAt                                     <  1:6848114/100‑1 (MQ=255)
                                                           atcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATa                                    <  2:2733914/100‑1 (MQ=255)
                                                           atcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATa                                    <  2:3319144/100‑1 (MQ=255)
                                                            tcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATaa                                   <  1:2544888/100‑1 (MQ=255)
                                                              acgacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGa                                 <  1:2644822/100‑1 (MQ=255)
                                                              acgacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGa                                 <  2:5155827/100‑1 (MQ=255)
                                                               cgacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGac                                <  1:2012865/100‑1 (MQ=255)
                                                                gacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGaca                               <  2:599133/100‑1 (MQ=255)
                                                                gacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGaca                               >  1:3487762/1‑100 (MQ=255)
                                                                 acgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGacac                              <  1:4549083/100‑1 (MQ=255)
                                                                 acgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGacac                              >  2:1542297/1‑100 (MQ=255)
                                                                       gATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTaa                        >  1:5646777/1‑100 (MQ=255)
                                                                        aTGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAc                       >  1:1629558/1‑100 (MQ=255)
                                                                        aTGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAc                       >  2:6673226/1‑100 (MQ=255)
                                                                        aTGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAc                       <  1:3046991/100‑1 (MQ=255)
                                                                          gAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGc                     >  2:6924669/1‑100 (MQ=255)
                                                                          gAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGc                     <  2:6870709/100‑1 (MQ=255)
                                                                           aaCCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCt                    >  1:825115/1‑100 (MQ=255)
                                                                           aaCCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAACACACGGGTAACGCt                    <  1:6940250/100‑1 (MQ=255)
                                                                           aaCCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACT‑GGGGATAAGACACGGGTAACGCTg                   >  1:3394316/1‑100 (MQ=255)
                                                                            aCCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTg                   >  1:4776092/1‑100 (MQ=255)
                                                                            aCCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTg                   <  2:3708112/100‑1 (MQ=255)
                                                                                 gaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTggg              >  1:1144670/1‑100 (MQ=255)
                                                                                 gaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTggg              <  1:584462/100‑1 (MQ=255)
                                                                                  aacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGc             >  2:2088484/1‑100 (MQ=255)
                                                                                     ggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAt          >  1:211420/1‑100 (MQ=255)
                                                                                     ggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAt          <  2:28104/100‑1 (MQ=255)
                                                                                      gaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAtt         >  2:4441704/1‑100 (MQ=255)
                                                                                      gaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAtt         >  1:514623/1‑100 (MQ=255)
                                                                                       aaaTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTa        <  2:863934/100‑1 (MQ=255)
                                                                                       aaaTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTa        >  2:4785592/1‑100 (MQ=255)
                                                                                       aaaTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTa        <  1:5996162/100‑1 (MQ=255)
                                                                                       aaaTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTa        <  1:4066830/100‑1 (MQ=255)
                                                                                        aaTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAt       >  1:1007665/1‑100 (MQ=255)
                                                                                        aaTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAt       <  1:3510290/100‑1 (MQ=255)
                                                                                         aTTTCAGGGGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATg      >  2:5851496/1‑100 (MQ=255)
                                                                                         aTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATg      <  1:897567/100‑1 (MQ=255)
                                                                                            tCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGccc   >  1:2872558/1‑100 (MQ=255)
                                                                                                |                                                                                                
TCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCA  >  NC_000913/4162050‑4162242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑