BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,472,857	A→G	intergenic (+117/‑28)	bdcR → / → tabA	transcriptional repressor for divergent bdcA/biofilm modulator regulated by toxins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,472,857	0	A	G	100.0%	1524.1 / NA	347	intergenic (+117/‑28)	bdcR/tabA	transcriptional repressor for divergent bdcA/biofilm modulator regulated by toxins
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (174/173); total (174/173)

Only 100 of 349 total aligned reads displayed.

GGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCAT  >  NC_000913/4472760‑4472953

 gCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgt                                                                                               <  2:3490724/100‑1 (MQ=255)

   gggAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgttg                                                                                             <  1:5694226/100‑1 (MQ=255)

    ggAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgttgt                                                                                            >  2:4709338/1‑100 (MQ=255)

     gAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgttgtt                                                                                           >  2:713883/1‑100 (MQ=255)

     gAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgttgtt                                                                                           <  2:2225752/100‑1 (MQ=255)

     gAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgttgtt                                                                                           >  2:1649779/1‑100 (MQ=255)

      aaaGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGttt                                                                                          >  1:1738504/1‑100 (MQ=255)

      aaaGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGttt                                                                                          <  1:6004182/100‑1 (MQ=255)

       aaGCCCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGtttt                                                                                         <  2:2694615/100‑1 (MQ=255)

        aGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTtta                                                                                        <  1:1002780/100‑1 (MQ=255)

        aGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTtta                                                                                        <  2:5915457/100‑1 (MQ=255)

         gcgcCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaa                                                                                       >  1:4426114/1‑100 (MQ=255)

         gcgcCTGCCGATAAGTTGTGATAAGACAATAACTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTGGTATAACGATAGAAATGAAGCGTTGTTttaa                                                                                       <  2:1289002/100‑1 (MQ=255)

          cgcCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaat                                                                                      <  1:7020298/100‑1 (MQ=255)

          cgcCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaat                                                                                      <  1:3495492/100‑1 (MQ=255)

                 cGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAg                                                                               >  1:1425936/1‑100 (MQ=255)

                 cGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAg                                                                               <  1:3127404/100‑1 (MQ=255)

                  gATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGt                                                                              <  1:916392/100‑1 (MQ=255)

                   aTAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTg                                                                             >  2:322991/1‑100 (MQ=255)

                    tAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTgg                                                                            >  1:3871437/1‑100 (MQ=255)

                    tAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTgg                                                                            >  1:4536656/1‑100 (MQ=255)

                     aaGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGa                                                                           <  1:4776122/100‑1 (MQ=255)

                       gTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGaaa                                                                         <  1:3842944/100‑1 (MQ=255)

                          gtgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAga                                                                      >  1:5466706/1‑100 (MQ=255)

                          gtgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAga                                                                      >  2:3979239/1‑100 (MQ=255)

                          gtgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAga                                                                      >  2:1538464/1‑100 (MQ=255)

                           tgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgat                                                                     >  2:5546979/1‑100 (MQ=255)

                           tgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgat                                                                     >  2:4625002/1‑100 (MQ=255)

                           tgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgat                                                                     <  2:232151/100‑1 (MQ=255)

                            gATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgatg                                                                    <  2:4323064/100‑1 (MQ=255)

                             aTAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgatga                                                                   <  1:5005351/100‑1 (MQ=255)

                              tAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCTTTGTTTTAATTAAGGAGTGGAAAAgatgat                                                                  >  1:4690093/1‑100 (MQ=255)

                              tAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgatgat                                                                  >  1:3337040/1‑100 (MQ=255)

                              tAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgatgat                                                                  <  1:6445876/100‑1 (MQ=255)

                               aaGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatc                                                                 <  1:2812626/100‑1 (MQ=255)

                               aaGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatc                                                                 >  2:3612833/1‑100 (MQ=255)

                                 gACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatcat                                                               <  1:2957477/100‑1 (MQ=255)

                                  aCAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatcatc                                                              <  1:6253584/100‑1 (MQ=255)

                                  aCAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatcatc                                                              <  1:769223/100‑1 (MQ=255)

                                   cAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCg                                                             >  1:1960076/1‑100 (MQ=255)

                                   cAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCg                                                             <  1:2637802/100‑1 (MQ=255)

                                   cAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCg                                                             >  1:6639830/1‑100 (MQ=255)

                                    aataatTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGATGTTTTAATTAAGGAGTGGAAAAGATGATCATCgg                                                            >  1:510423/1‑100 (MQ=255)

                                       aatTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGaaa                                                         <  2:2057621/100‑1 (MQ=255)

                                        atTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAat                                                        <  1:2084260/100‑1 (MQ=255)

                                        atTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAat                                                        >  1:3025438/1‑100 (MQ=255)

                                        atTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAat                                                        <  2:4019130/100‑1 (MQ=255)

                                          tCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAatat                                                      <  1:6546741/100‑1 (MQ=255)

                                          tCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAatat                                                      <  1:695560/100‑1 (MQ=255)

                                               cgTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATa                                                 >  2:668139/1‑100 (MQ=255)

                                                gTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATaa                                                <  2:1912402/100‑1 (MQ=255)

                                                 ttAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAAt                                               >  2:881244/1‑100 (MQ=255)

                                                  tAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATc                                              >  2:3795738/1‑100 (MQ=255)

                                                    aGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCtt                                            <  2:6410267/100‑1 (MQ=255)

                                                     ggCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCtta                                           >  2:1454085/1‑99 (MQ=255)

                                                     ggCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTc                                           >  1:7147740/1‑100 (MQ=255)

                                                          gCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCg                                      <  2:1803195/100‑1 (MQ=255)

                                                          gCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCg                                      >  2:5353502/1‑100 (MQ=255)

                                                             gAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTgg                                   >  2:2516381/1‑100 (MQ=255)

                                                              aaTTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGc                                  >  1:1104831/1‑100 (MQ=255)

                                                              aaTTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGc                                  <  2:2938979/100‑1 (MQ=255)

                                                                ttGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTa                                >  2:69244/1‑100 (MQ=255)

                                                                ttGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTa                                >  2:578157/1‑100 (MQ=255)

                                                                  gATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAcc                              >  1:5659514/1‑100 (MQ=255)

                                                                  gATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAcc                              <  2:4424486/100‑1 (MQ=255)

                                                                  gATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAcc                              >  1:1854810/1‑100 (MQ=255)

                                                                   aTTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAccc                             >  1:3566872/1‑100 (MQ=255)

                                                                   aTTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAccc                             >  1:4070246/1‑100 (MQ=255)

                                                                   aTTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAccc                             <  1:5330584/100‑1 (MQ=255)

                                                                    ttATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAcccc                            >  2:5041016/1‑100 (MQ=255)

                                                                       tCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAgg                         <  2:2126562/100‑1 (MQ=255)

                                                                         ttttCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAg                       >  2:6312385/1‑100 (MQ=255)

                                                                          tttCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGt                      <  1:4324470/100‑1 (MQ=255)

                                                                           ttCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGtt                     <  1:1649779/100‑1 (MQ=255)

                                                                           ttCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGtt                     >  1:2368071/1‑100 (MQ=255)

                                                                            tCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTa                    <  1:6802080/100‑1 (MQ=255)

                                                                            tCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTa                    >  1:4836789/1‑100 (MQ=255)

                                                                             cGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTAc                   >  2:3093761/1‑100 (MQ=255)

                                                                              gTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACg                  >  1:1048315/1‑100 (MQ=255)

                                                                              gTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACg                  >  2:482599/1‑100 (MQ=255)

                                                                              gTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACg                  >  1:2267915/1‑100 (MQ=255)

                                                                               tataACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGc                 >  2:5217892/1‑100 (MQ=255)

                                                                               tataACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGc                 >  1:61532/1‑100 (MQ=255)

                                                                               tataACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGc                 <  2:3362269/100‑1 (MQ=255)

                                                                               tataACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGc                 <  1:437695/100‑1 (MQ=255)

                                                                                ataACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGcc                <  2:1238914/100‑1 (MQ=255)

                                                                                 taACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCa               >  2:5814642/1‑100 (MQ=255)

                                                                                 taACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCa               >  1:4168687/1‑100 (MQ=255)

                                                                                 taACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGAACATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCa               >  2:6863384/1‑100 (MQ=255)

                                                                                  aaCGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAg              <  2:5188169/100‑1 (MQ=255)

                                                                                      aTAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGa          >  1:3715780/1‑100 (MQ=255)

                                                                                      aTAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGa          <  2:6527434/100‑1 (MQ=255)

                                                                                       tAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGAt         >  2:6289504/1‑100 (MQ=255)

                                                                                       tAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGAt         >  1:2453727/1‑100 (MQ=255)

                                                                                        aGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGAtt        >  1:4575847/1‑100 (MQ=255)

                                                                                        aGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGAtt        <  2:6049580/100‑1 (MQ=255)

                                                                                         gAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTg       >  1:3677028/1‑100 (MQ=255)

                                                                                           aaTGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAg     <  2:6768500/100‑1 (MQ=255)

                                                                                            aTGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGc    >  1:5861932/1‑100 (MQ=255)

                                                                                             tGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCa   <  1:133052/100‑1 (MQ=255)

GGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCAT  >  NC_000913/4472760‑4472953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`