BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,344	0	G	A	49.7%	48.4 / inf	467	Q163Q (CAG→CAA)	yecD	isochorismatase family protein
Reads supporting (aligned to +/- strand): ref base G (116/119); new base A (115/117); total (231/236)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 508 total aligned reads displayed.

TATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTA  >  NC_000913/1949251‑1949436

tatCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGTCGCCAGCGCCGagcag                                                                                              >  1:582425/1‑94 (MQ=255)

 atCGGTGTTTAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCTGAAGACGCCTGTAGTGCCGCCAGCGCTGagcaga                                                                                             >  1:4229536/1‑93 (MQ=255)

      tgtTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAAt                                                                                        <  1:6028327/94‑1 (MQ=255)

        ttGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATat                                                                                      >  1:5740652/1‑93 (MQ=255)

           taTCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAg                                                                                   <  1:5339343/93‑1 (MQ=255)

            aTCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAgc                                                                                  >  1:5056013/1‑93 (MQ=255)

             tCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAgcc                                                                                 >  1:1839984/1‑92 (MQ=255)

             accACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAgca                                                                                 <  1:689940/93‑3 (MQ=255)

                aCCGCCCGCAGTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGcaca                                                                                          <  2:6679346/82‑1 (MQ=255)

                 ccgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCaa                                                                             >  1:4792224/1‑94 (MQ=255)

                  cgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAAt                                                                                        <  2:1316072/82‑1 (MQ=255)

                   gcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATa                                                                                       <  2:1673077/82‑1 (MQ=255)

                      cgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACa                                                                                    >  2:3652536/1‑82 (MQ=255)

                      cgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCata                                                                        <  1:1651641/94‑1 (MQ=255)

                      cgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCata                                                                        >  1:4584670/1‑94 (MQ=255)

                       tcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCatat                                                                       <  1:2416308/93‑1 (MQ=255)

                       gcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAg                                                                                   >  2:5979633/1‑82 (MQ=255)

                       gcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAg                                                                                   <  2:7101588/82‑1 (MQ=255)

                       gcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAg                                                                                   >  2:2353246/1‑82 (MQ=255)

                        cAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCatatt                                                                      >  1:2266610/1‑93 (MQ=255)

                         aaTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATcc                                                                     >  1:7182221/1‑93 (MQ=255)

                          aTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCtc                                                                    >  1:6989427/1‑93 (MQ=255)

                          aTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTa                                                                    >  1:1053100/1‑94 (MQ=255)

                            tccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCTCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTAcc                                                                  <  1:5246314/93‑1 (MQ=255)

                            gCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCa                                                                              >  2:7432640/1‑82 (MQ=255)

                              cTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAAc                                                                            <  2:1903634/82‑1 (MQ=255)

                              cTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTaat                                                                            >  2:3556104/1‑81 (MQ=255)

                               tGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAAcc                                                                           <  2:4659915/82‑1 (MQ=255)

                                gggAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCa                                                                          >  2:2979051/1‑82 (MQ=255)

                                gggAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCa                                                                          <  2:4779102/82‑1 (MQ=255)

                                 ggAACTTGGCTTTAATCTGGTGATTGCCGACCACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCat                                                                         >  2:3731053/1‑82 (MQ=255)

                                  taaCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCa                                                            <  1:6463790/93‑1 (MQ=255)

                                   aaCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCatat                                                                       <  2:4479067/82‑1 (MQ=255)

                                    aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATc                                                                      >  2:5252712/1‑82 (MQ=255)

                                    aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCAtg                                                          >  1:3989540/1‑93 (MQ=255)

                                    aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCAta                                                          >  1:6829486/1‑93 (MQ=255)

                                      ttGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGc                                                        >  1:1466355/1‑94 (MQ=255)

                                      ctGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGc                                                        <  1:3420287/93‑1 (MQ=255)

                                        tgCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGccc                                                      <  1:7343432/93‑1 (MQ=255)

                                        ggCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTAcc                                                                  <  2:3344251/82‑1 (MQ=255)

                                          cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCcg                                                                >  2:3897996/1‑82 (MQ=255)

                                          cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgt                                                    <  1:1013754/94‑1 (MQ=255)

                                           tttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCgtg                                                   <  1:6137612/94‑1 (MQ=255)

                                           tttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCgtc                                                   >  1:4963957/1‑93 (MQ=255)

                                             tAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCgcgc                                                             <  2:4396087/82‑1 (MQ=255)

                                              aaTCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCa                                                            >  2:5581209/1‑82 (MQ=255)

                                               ttCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCg                                               <  1:2064931/93‑1 (MQ=255)

                                                 cTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCg                                                         <  2:5309758/82‑1 (MQ=255)

                                                 cTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGtc                                             >  1:7068614/1‑93 (MQ=255)

                                                   ggTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGcc                                                       >  2:5055540/1‑82 (MQ=255)

                                                    ctGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCg                                          <  1:2566487/93‑1 (MQ=255)

                                                     ggATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGt                                         <  1:780999/93‑1 (MQ=255)

                                                     agATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGt                                         <  1:3850127/93‑1 (MQ=255)

                                                        ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTAGCCGCGCATCGCCCgtgt                                                  >  2:5453056/1‑82 (MQ=255)

                                                        ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCgtgt                                                  >  2:4144551/1‑82 (MQ=255)

                                                        ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTggc                                      >  1:5637835/1‑93 (MQ=255)

                                                        ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtgt                                                  >  2:6337054/1‑82 (MQ=255)

                                                        ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgggt                                                  <  2:7247039/82‑1 (MQ=255)

                                                          gCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGc                                                <  2:2627663/82‑1 (MQ=255)

                                                          gCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGc                                                <  2:828062/82‑1 (MQ=255)

                                                             gAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTa                                             <  2:3423495/82‑1 (MQ=255)

                                                             gAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAgaga                                 <  1:1768629/94‑1 (MQ=255)

                                                             gAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAagagc                                 >  1:1550659/1‑93 (MQ=255)

                                                             gAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAgaga                                 <  1:2712907/94‑1 (MQ=255)

                                                              aaGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGAt                                >  1:4542589/1‑94 (MQ=255)

                                                               aGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGAta                               >  1:1259093/1‑93 (MQ=255)

                                                               aGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGc                                           <  2:1554717/82‑1 (MQ=255)

                                                               aGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATc                               >  1:6337859/1‑94 (MQ=255)

                                                                gACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATcg                              >  1:1410932/1‑93 (MQ=255)

                                                                gACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCg                                          <  2:2867197/82‑1 (MQ=255)

                                                                gACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATca                              >  1:439892/1‑93 (MQ=255)

                                                                   gCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                       <  2:4049702/82‑1 (MQ=255)

                                                                     cTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcat                         >  1:6656417/1‑90 (MQ=255)

                                                                     cTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcac                         >  1:2964462/1‑90 (MQ=255)

                                                                      tGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAag                                    >  2:2078783/1‑82 (MQ=255)

                                                                          ttGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGtt                    <  1:6564610/93‑1 (MQ=255)

                                                                          gTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGAt                                <  2:6146593/82‑1 (MQ=255)

                                                                          ctGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGtt                    <  1:4066155/93‑1 (MQ=255)

                                                                             ccgccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTAta                 >  1:88856/1‑93 (MQ=255)

                                                                               gccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCa                           <  2:2452366/82‑1 (MQ=255)

                                                                               gccAGCGCCGAGCAACACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGTTCCTCa                           <  2:4973611/82‑1 (MQ=255)

                                                                               cccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAt               <  1:3288801/93‑1 (MQ=255)

                                                                                tcAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAtt              <  1:5533252/93‑1 (MQ=255)

                                                                                  aGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAcg                        <  2:5181610/82‑1 (MQ=255)

                                                                                  aGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAcg                        >  2:3185657/1‑82 (MQ=255)

                                                                                  aGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAtttt            >  1:4330245/1‑93 (MQ=255)

                                                                                   gcgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCAcgc                       >  2:1779373/1‑82 (MQ=255)

                                                                                    tgcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACa          <  1:5452965/93‑1 (MQ=255)

                                                                                     gcCGAGCAACACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGt                     >  2:5730948/1‑82 (MQ=255)

                                                                                     gcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACAt         <  1:5974690/94‑1 (MQ=255)

                                                                                      ccGAGCAGCACAATAGCAGTATCAACCATATCTCCCCGCGCATCGCACGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGtt                    <  2:6483559/82‑1 (MQ=255)

                                                                                      ccGAGCAGCACAATAACAGTATCAACCATATCTCCCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGtt                    <  2:1207837/82‑1 (MQ=255)

                                                                                      ccGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGtt                    >  2:3102507/1‑82 (MQ=255)

                                                                                      ccGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGtt                    >  2:2744013/1‑82 (MQ=255)

                                                                                       cGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTa                   >  2:1061438/1‑82 (MQ=255)

                                                                                        gAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCga      >  1:3659541/1‑93 (MQ=255)

                                                                                         agcagcACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGt     >  1:3163755/1‑94 (MQ=255)

                                                                                          gcagcaCAATAACAGTATCAACCATATCTACCCGCGCAGCGCCCGTGTGCGTAGCGTGGAAGATATCCTCAACGCGTTATGATTTACATCGGTc    >  1:7465167/1‑94 (MQ=255)

                                                                                          gcaacaCAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGtt    >  1:3732649/1‑93 (MQ=255)

                                                                                           cagcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGAt               <  2:6874902/82‑1 (MQ=255)

TATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTA  >  NC_000913/1949251‑1949436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`