BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,362	0	C	T	46.1%	263.9 / inf	527	N169N (AAC→AAT)	yecD	isochorismatase family protein
Reads supporting (aligned to +/- strand): ref base C (134/150); new base T (118/125); total (252/275)
Fisher's exact test for biased strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-04
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 547 total aligned reads displayed.

AATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGT  >  NC_000913/1949262‑1949462

 aTCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAAcc                                                                                                     <  2:1673999/101‑1 (MQ=255)

    cACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCata                                                                                                  <  1:2918019/101‑1 (MQ=255)

    cACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCata                                                                                                  <  2:2539031/101‑1 (MQ=255)

     aCCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCatat                                                                                                 >  1:6491149/1‑101 (MQ=255)

     aCCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCatat                                                                                                 <  1:6407331/101‑1 (MQ=255)

      ccgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATc                                                                                                <  1:3398482/101‑1 (MQ=255)

       cgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCt                                                                                               <  2:4267035/101‑1 (MQ=255)

        gcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTa                                                                                              >  1:6274929/1‑101 (MQ=255)

           cncANNGCCTGGGANCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTAcca                                                                                           >  2:1147034/1‑100 (MQ=255)

           cgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTAccc                                                                                           <  2:6492606/101‑1 (MQ=255)

           cgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTAccc                                                                                           >  2:6365148/1‑101 (MQ=255)

            gcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCcg                                                                                          <  1:2672875/101‑1 (MQ=255)

             cAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCcgc                                                                                         >  2:101108/1‑101 (MQ=255)

              aaTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCcgcg                                                                                        >  2:4640169/1‑101 (MQ=255)

              aaTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCcgcg                                                                                        >  1:1923699/1‑101 (MQ=255)

              aaTGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCcgcg                                                                                        >  2:5424122/1‑101 (MQ=255)

                tGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCa                                                                                      >  1:4962771/1‑101 (MQ=255)

                 gCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCgcgcct                                                                                     >  2:6587441/1‑99 (MQ=255)

                 gCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCac                                                                                     >  1:1472712/1‑100 (MQ=255)

                 gCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCAt                                                                                     <  2:2726273/101‑1 (MQ=255)

                  ccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATc                                                                                    <  1:5846583/101‑1 (MQ=255)

                  ccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATc                                                                                    <  2:3967490/101‑1 (MQ=255)

                  ccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATc                                                                                    <  2:2222588/101‑1 (MQ=255)

                  ccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATc                                                                                    <  1:6039610/101‑1 (MQ=255)

                   cTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCg                                                                                   <  2:5017726/101‑1 (MQ=255)

                   cTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCg                                                                                   <  1:4201821/101‑1 (MQ=255)

                    tGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGc                                                                                  <  2:2388235/101‑1 (MQ=255)

                      ggAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTCCCCGCGCATCGccc                                                                                <  2:4553941/101‑1 (MQ=255)

                          cTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtgt                                                                            >  1:4116161/1‑101 (MQ=255)

                          cTTGGCTTTAATCTGGTGATTGCCGAAAACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATGTACCCGCGCATCGCCCgtgg                                                                            >  2:2009737/1‑100 (MQ=255)

                              gCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGCGTGCga                                                                        >  2:5296612/1‑100 (MQ=255)

                              ccTTTAATCTGGTGATTGCCGAAGACGCCTGTACTGCCGCCAGCGCCGAGCAGAACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGt                                                                        <  2:3259558/100‑1 (MQ=255)

                               cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTa                                                                       <  1:6961274/101‑1 (MQ=255)

                               cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTa                                                                       <  2:875547/101‑1 (MQ=255)

                               cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTa                                                                       <  2:5561574/101‑1 (MQ=255)

                               cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTa                                                                       <  2:5819894/101‑1 (MQ=255)

                               cTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTa                                                                       <  2:1417048/101‑1 (MQ=255)

                                tttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAg                                                                      <  2:4492299/101‑1 (MQ=255)

                                 ttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGc                                                                     >  1:2748603/1‑101 (MQ=255)

                                   aaTCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGt                                                                   >  1:2082116/1‑101 (MQ=255)

                                   aaTCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGt                                                                   >  2:5060285/1‑101 (MQ=255)

                                     tCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                                                 <  1:4724230/101‑1 (MQ=255)

                                     tCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                                                 <  2:2916784/101‑1 (MQ=255)

                                     tCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                                                 >  1:6673810/1‑101 (MQ=255)

                                      cTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGa                                                                >  1:2562648/1‑101 (MQ=255)

                                       tGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGaa                                                               >  1:3800340/1‑101 (MQ=255)

                                        ggTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAag                                                              >  1:2287861/1‑101 (MQ=255)

                                          tGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAagag                                                            >  1:3231428/1‑101 (MQ=255)

                                           gATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAgaga                                                           >  1:923738/1‑101 (MQ=255)

                                           gATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAgaga                                                           <  1:1547546/101‑1 (MQ=255)

                                            aTTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGAt                                                          <  1:4906336/101‑1 (MQ=255)

                                              tGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATcc                                                        <  1:3633754/101‑1 (MQ=255)

                                              tGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCg                                                                                   >  1:2413502/1‑74 (MQ=255)

                                                ccGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTc                                                      <  1:5602951/101‑1 (MQ=255)

                                                 cGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttc                                                     >  1:3583853/1‑99 (MQ=255)

                                                   aaGGCGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcac                                                   <  1:1661427/101‑5 (MQ=255)

                                                   aaGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCaan                                                   >  2:2444772/1‑100 (MQ=255)

                                                    aGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcacg                                                  <  1:706604/101‑6 (MQ=255)

                                                     caCGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAcgc                                                 <  2:7023059/100‑1 (MQ=255)

                                                       cGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGt                                               <  1:4433889/101‑1 (MQ=255)

                                                         ccTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTa                                             <  1:6807557/101‑1 (MQ=255)

                                                          cTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTAt                                            <  1:774640/101‑1 (MQ=255)

                                                          cTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTAt                                            <  1:216948/101‑1 (MQ=255)

                                                           tGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGGATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGCGGAAGAGATCCTCAACGCGTTATg                                           >  2:3084124/1‑101 (MQ=255)

                                                              aGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAtt                                        <  2:6103617/101‑1 (MQ=255)

                                                                  ccgccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACa                                    <  1:6171526/101‑1 (MQ=255)

                                                                   cgccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACAt                                   <  2:1202818/101‑1 (MQ=255)

                                                                    gccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATc                                  <  1:5313377/101‑1 (MQ=255)

                                                                        gcgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTc                              <  1:176857/101‑1 (MQ=255)

                                                                        gcgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTc                              <  1:502501/101‑1 (MQ=255)

                                                                         cgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCt                             <  2:6274929/101‑1 (MQ=255)

                                                                          gcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGTTCCTTCACGCGTTATGATTTACATCGGTCtg                            >  1:1352578/1‑100 (MQ=255)

                                                                          gcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCtg                            >  2:945253/1‑100 (MQ=255)

                                                                            cGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTAcc                          >  2:3748571/1‑101 (MQ=255)

                                                                            cGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTAcc                          >  1:3967931/1‑101 (MQ=255)

                                                                            cGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTAcc                          <  1:5151260/101‑1 (MQ=255)

                                                                             gAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCg                         >  2:5493594/1‑101 (MQ=255)

                                                                              agcagcACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGc                        <  1:5796083/101‑1 (MQ=255)

                                                                                cagcaCAATAACAGTAGCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCaa                      >  2:6840749/1‑101 (MQ=255)

                                                                                 aacacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAAt                     <  2:4347273/99‑1 (MQ=255)

                                                                                   cacaATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATgg                   <  2:3018581/101‑1 (MQ=255)

                                                                                    acaATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGt                  >  1:5936939/1‑101 (MQ=255)

                                                                                        aaaCAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCa              <  2:3828321/100‑1 (MQ=255)

                                                                                         aaCAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCAt             >  1:1282433/1‑101 (MQ=255)

                                                                                          aCAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATc            >  2:5214339/1‑101 (MQ=255)

                                                                                          aCAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATc            >  1:4350477/1‑101 (MQ=255)

                                                                                          aCAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATc            <  2:1785952/101‑1 (MQ=255)

                                                                                          aCAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATc            >  2:4884689/1‑101 (MQ=255)

                                                                                          aCAGCATTAATCATATCTACCCGCGCATCGCCCGGGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCGGTCTGCCGCAATGGTCGCATc            <  2:1898580/101‑1 (MQ=255)

                                                                                           cAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATcc           <  1:4582884/101‑1 (MQ=255)

                                                                                            aGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCt          >  2:584206/1‑101 (MQ=255)

                                                                                            aGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCt          <  1:3462859/101‑1 (MQ=255)

                                                                                             gTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTCGCGTGGAAGAGATCCTCAACGAGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTa         >  1:2877784/1‑101 (MQ=255)

                                                                                             gTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTa         <  2:4062072/101‑1 (MQ=255)

                                                                                             atttCACCCATATCTACCCGCGCATCGCCCGTGGGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCACATCCTa         <  1:387878/98‑1 (MQ=255)

                                                                                              tATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTaa        <  1:5406985/101‑1 (MQ=255)

                                                                                              tATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTaa        >  2:3491690/1‑101 (MQ=255)

                                                                                                tCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAAt      <  1:1147034/101‑1 (MQ=255)

                                                                                                tCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAAt      <  1:1004725/101‑1 (MQ=255)

                                                                                                  aaCCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATgg    >  2:5971276/1‑101 (MQ=255)

AATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTACCGCAATGGTCGCATCCTAAATGGGT  >  NC_000913/1949262‑1949462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`