Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,5180TC52.0% 168.6 / 275.2 177D154D (GAT→GAC) nohDDLP12 prophage; DNA packaging protein
Reads supporting (aligned to +/- strand):  ref base T (47/38);  new base C (47/45);  total (94/83)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 186 total aligned reads displayed.

CCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTT  >  NC_000913/580452‑580618
                                                                  |                                                                                                    
tcctcTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTgg                                                                    >  1:1336976/5‑101 (MQ=0)
tcctcTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTgg                                                                    <  1:2814133/97‑1 (MQ=0)
tcctcTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTgg                                                                    >  1:3861989/5‑101 (MQ=0)
tcctcTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTgg                                                                    <  1:1602109/97‑1 (MQ=0)
 cctcTGTCGGTTCAGCGGCGCTTCCCGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGa                                                                   >  1:5618320/4‑101 (MQ=0)
                     cttcccGGAGCTGGAAAACCGACATATTGCTTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGc                                               >  2:2851550/5‑101 (MQ=0)
                     cttcccGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGc                                               >  2:1701065/5‑101 (MQ=0)
                     cttcccGGAGCTGGAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCCGCGCTGGATGAAATAATACCGGGGTTGc                                               >  1:3971805/5‑101 (MQ=1)
                                  gAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAatatat                                  <  2:459295/101‑1 (MQ=11)
                                  gAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAatatat                                  >  1:818442/1‑101 (MQ=11)
                                  gAAAACCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAatatat                                  <  2:4949138/101‑1 (MQ=11)
                                   aaaaCCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATc                                 <  2:1988320/101‑1 (MQ=31)
                                   aaaaCCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATc                                 <  1:1709959/101‑1 (MQ=11)
                                   aaaaCCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATc                                 <  2:5428262/101‑1 (MQ=11)
                                    aaaCCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCg                                <  1:723133/101‑1 (MQ=11)
                                     aaCCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGa                               >  1:3015545/1‑101 (MQ=11)
                                     aaCCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGa                               >  2:2706289/1‑101 (MQ=11)
                                     aaCCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGa                               >  1:6167688/1‑101 (MQ=11)
                                      aCCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGaa                              >  1:5548232/1‑101 (MQ=35)
                                      aCCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGaa                              <  1:4939168/101‑1 (MQ=11)
                                      aCCGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGaa                              >  1:1983047/1‑101 (MQ=11)
                                       ccGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAAc                             >  2:5336162/1‑101 (MQ=11)
                                       ccGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGATGCTGAGTGAATATATCGaat                             >  2:705664/1‑100 (MQ=11)
                                        cNACNNGTTGATTTNCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAAct                            >  2:1124158/1‑100 (MQ=255)
                                        cGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACa                            >  1:1385385/1‑101 (MQ=38)
                                        cGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACa                            <  1:1536604/101‑1 (MQ=38)
                                        cGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACa                            >  1:604824/1‑101 (MQ=38)
                                        cGACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACa                            >  1:3842567/1‑101 (MQ=11)
                                        cGACATATTGATTTACTCAAGAAGGACATCATTAAAGCCGAGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGAGTTGCTGAGTGAATATATCGAAct                            >  2:3314966/1‑100 (MQ=2)
                                         gACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAg                           >  2:6760064/1‑101 (MQ=38)
                                         gACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAg                           >  1:1637257/1‑101 (MQ=38)
                                         gACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAg                           >  2:5383117/1‑101 (MQ=38)
                                         gACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAg                           <  2:2562705/101‑1 (MQ=38)
                                         gACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAg                           <  2:26144/101‑1 (MQ=14)
                                         gACATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCGGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAg                           <  1:2495019/101‑1 (MQ=14)
                                          aCATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGt                          <  1:1190126/101‑1 (MQ=40)
                                          aCATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGt                          >  2:5505751/1‑101 (MQ=9)
                                          aCATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGt                          >  2:5130946/1‑101 (MQ=14)
                                          aCATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGt                          >  1:5139002/1‑101 (MQ=14)
                                          aCATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGt                          >  1:2374357/1‑101 (MQ=14)
                                           cATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTc                         <  2:2355169/101‑1 (MQ=40)
                                           cATATTGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTc                         <  2:598525/101‑1 (MQ=14)
                                            aTGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCa                        >  1:6975672/1‑101 (MQ=255)
                                             tattGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAg                       >  2:3528753/3‑101 (MQ=14)
                                             tattGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAg                       <  1:592119/99‑1 (MQ=14)
                                             tattGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAg                       <  1:376161/99‑1 (MQ=14)
                                             tattGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAg                       <  2:6598309/99‑1 (MQ=14)
                                             tattGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAg                       <  1:1100005/99‑1 (MQ=14)
                                             tGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAg                       <  1:4194863/101‑1 (MQ=255)
                                              gTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAgg                      <  2:6471093/101‑1 (MQ=255)
                                              gTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAgg                      >  2:2354059/1‑101 (MQ=255)
                                              attGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAgg                      <  2:3944054/100‑1 (MQ=14)
                                              attGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAgg                      >  2:6255237/2‑101 (MQ=14)
                                               ttGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGt                     <  2:4284802/101‑1 (MQ=255)
                                               ttGATTTCCTCAAGAAGGACATCATTAAAGCCATGATCAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTTAATATATCGATCCGTCAGGt                     <  2:5190463/101‑1 (MQ=9)
                                               ttGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGt                     <  1:2582601/101‑1 (MQ=14)
                                                tGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGtt                    <  1:5850247/101‑1 (MQ=255)
                                                tGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGtt                    >  2:2308063/1‑101 (MQ=255)
                                                tGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATATCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGtt                    >  1:2637179/1‑101 (MQ=14)
                                                tGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGtt                    <  1:4905975/101‑1 (MQ=14)
                                                tGATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGAGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGtt                    >  2:1621258/1‑101 (MQ=14)
                                                 gATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTa                   >  2:4825945/1‑101 (MQ=255)
                                                 gATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTa                   <  1:3125118/101‑1 (MQ=255)
                                                 gATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTa                   <  1:2539804/101‑1 (MQ=255)
                                                 gATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTa                   >  2:937329/1‑101 (MQ=17)
                                                 gATTTCCTCAAGAAGGACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTa                   <  1:967391/101‑1 (MQ=17)
                                                  aTTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTaa                  >  2:887219/1‑101 (MQ=255)
                                                  aTTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTaa                  >  2:1596893/1‑101 (MQ=255)
                                                  aTTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTaa                  >  1:4427366/1‑101 (MQ=255)
                                                  aTTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTaa                  >  1:5930770/1‑101 (MQ=255)
                                                   tttCCTGAAACGGGATATCATCAAAGCCATGAGCAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAc                 >  2:2167906/1‑101 (MQ=255)
                                                    ttCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACa                <  2:2983/101‑1 (MQ=255)
                                                    ttCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACa                >  2:2828382/1‑101 (MQ=255)
                                                    tgccTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACa                <  2:2634664/99‑1 (MQ=255)
                                                      ccTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAgg              <  1:5071082/101‑1 (MQ=255)
                                                      ccTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAgg              <  1:4953126/101‑1 (MQ=255)
                                                       cTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGc             >  2:2885619/1‑101 (MQ=255)
                                                        tGAAACGGGATATCATCAAAGCCATGAGCAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGCGTGAATATATCTAACAGTCAGGTTAACAgggt            <  2:6960285/101‑3 (MQ=17)
                                                        tGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCt            >  1:3359042/1‑101 (MQ=255)
                                                        tGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCt            >  1:3575998/1‑101 (MQ=255)
                                                          aagaaggACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGc          >  2:6477440/6‑101 (MQ=17)
                                                          aagaaggACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGc          >  1:638413/6‑101 (MQ=17)
                                                          aaaCGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGc          >  2:6052403/1‑101 (MQ=255)
                                                           aaCGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCg         <  1:3674642/101‑1 (MQ=255)
                                                           aaCGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCg         <  1:3273531/101‑1 (MQ=255)
                                                            gaaggACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGcag        <  1:6953260/98‑3 (MQ=17)
                                                            caaggACATCATTAAAGCCATGAACAAAGCAGCTGAGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGNTTAAGGGGNNGCng        <  2:1141191/98‑1 (MQ=17)
                                                            aCGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTGAACAGGCTGCgg        <  2:6238118/101‑1 (MQ=255)
                                                             cGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGc       >  1:4266748/1‑101 (MQ=255)
                                                             aaggACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTGCAGc       <  1:1022689/99‑1 (MQ=17)
                                                              aggACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagca      <  1:2105908/100‑1 (MQ=17)
                                                              aggACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGATGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagca      <  1:1666857/100‑1 (MQ=17)
                                                               ggATATCATCAAAGCCATGAACAAAGGAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCAt     >  1:6852440/1‑101 (MQ=255)
                                                               ggATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCAt     >  1:4124614/1‑101 (MQ=255)
                                                               ggACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcac     >  2:3571666/1‑100 (MQ=17)
                                                               ggACATCATTAAAGCCATGAACAAAGCAGCTGCGCTGGATGAAATAATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAGGGGCTgcagcac     >  1:179210/1‑100 (MQ=17)
                                                                gATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCAtt    <  1:1886767/101‑1 (MQ=255)
                                                                 atatCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCAttt   >  1:645668/1‑101 (MQ=255)
                                                                 atatCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCAttt   >  2:2246420/1‑101 (MQ=255)
                                                                  tatCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCAtttt  <  1:2144243/101‑1 (MQ=255)
                                                                  |                                                                                                    
CCCCCTGTCGGTGCAGCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTT  >  NC_000913/580452‑580618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑