Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,842,032 G→T R67R (CGC→CGA)  hycG ← hydrogenase 3 and formate hydrogenase complex, HycG subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,842,0320GT99.6% 1013.5 / NA 257R67R (CGC→CGA) hycGhydrogenase 3 and formate hydrogenase complex, HycG subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (147/109);  total (148/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 277 total aligned reads displayed.

CACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACGTT  >  NC_000913/2841934‑2842129
                                                                                                  |                                                                                                 
cacgcacgCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCg                                                                                                 <  1:6661174/101‑1 (MQ=255)
   gcacgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTc                                                                                              >  2:6349492/1‑101 (MQ=255)
     acgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTg                                                                                            <  1:3051314/101‑1 (MQ=255)
       gcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGCCGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                                          <  1:6557732/101‑1 (MQ=255)
       gcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                                          >  1:3647102/1‑101 (MQ=255)
        cagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCAt                                                                                         <  2:1871251/101‑1 (MQ=255)
            gctggggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGAAAAATATCCGCATGACGCGGCGAAGGAACGATTTTAATGCCGAAAAGGTCTGCATCaaa                                                                                     >  2:4414498/4‑101 (MQ=255)
            gcagGNGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCaaa                                                                                     >  1:2629938/1‑101 (MQ=255)
            gcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCaaa                                                                                     <  2:3701949/101‑1 (MQ=255)
             cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                                    >  1:1913953/1‑101 (MQ=255)
             cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                                    <  1:1012826/101‑1 (MQ=255)
             cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                                    <  1:4343656/101‑1 (MQ=255)
              agGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACa                                                                                   <  1:5505500/101‑1 (MQ=255)
               ggggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAg                                                                                  >  2:1030511/1‑101 (MQ=255)
               ggggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAg                                                                                  >  1:73284/1‑101 (MQ=255)
                 gtaTCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcg                                                                                >  1:3475742/3‑101 (MQ=255)
                 ggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcg                                                                                <  2:2714807/101‑1 (MQ=255)
                 ggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcg                                                                                <  2:6307714/101‑1 (MQ=255)
                  ggtCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcgg                                                                               >  1:6994943/3‑101 (MQ=255)
                  gATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcgg                                                                               <  2:6080339/101‑1 (MQ=255)
                   aTCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcggc                                                                              <  2:4952086/101‑1 (MQ=255)
                   aTCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcggc                                                                              <  2:420831/101‑1 (MQ=255)
                     cGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGa                                                                            >  1:2263022/1‑101 (MQ=255)
                        aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAg                                                                         <  1:1106805/101‑1 (MQ=255)
                            cACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                                     >  2:4060242/1‑101 (MQ=255)
                             aCGGGTGACCGCGCCGGTAAACAGTTAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCCTTCTGCATCAAACAGCGGCGAAAGCGTgg                                                                    <  2:3449080/101‑1 (MQ=255)
                             aCGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTgg                                                                    >  2:841925/1‑101 (MQ=255)
                              cGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGc                                                                   >  2:5285398/1‑101 (MQ=255)
                              cGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGc                                                                   <  2:4753716/101‑1 (MQ=255)
                               gggTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCg                                                                  >  1:4186534/1‑101 (MQ=255)
                               gggTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCg                                                                  <  2:718512/101‑1 (MQ=255)
                                 gTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaa                                                                >  2:5086475/1‑101 (MQ=255)
                                   gACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaaa                                                              >  1:2945359/1‑101 (MQ=255)
                                   gACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaaa                                                              >  1:6419314/1‑101 (MQ=255)
                                     ccGCGCCGGTAAACAGGAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatt                                                            >  2:3279514/1‑101 (MQ=255)
                                      cgcgCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAattt                                                           >  1:2096039/1‑101 (MQ=255)
                                         gcCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcga                                                        >  1:2882938/1‑101 (MQ=255)
                                          ccGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgat                                                       >  1:1647887/1‑101 (MQ=255)
                                           cGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatt                                                      >  2:6661101/1‑101 (MQ=255)
                                            ggTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgattt                                                     >  1:5437755/1‑101 (MQ=255)
                                             gTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatttc                                                    <  2:5669067/101‑1 (MQ=255)
                                              tAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatttcg                                                   >  2:2457399/1‑101 (MQ=255)
                                                aaCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCa                                                 >  2:328315/1‑101 (MQ=255)
                                                 aCAGTAAAATATCCGCATGCCGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCaa                                                <  2:1686560/101‑1 (MQ=255)
                                                 aCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCaa                                                >  2:5164431/1‑101 (MQ=255)
                                                  cAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                               >  2:4617975/1‑101 (MQ=255)
                                                   aGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAcc                                              <  2:2260277/101‑1 (MQ=255)
                                                   aGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAcc                                              <  1:1809803/101‑1 (MQ=255)
                                                    gTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCg                                             <  2:6557031/101‑1 (MQ=255)
                                                      aaaaTCTCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGtt                                           <  2:375533/101‑1 (MQ=255)
                                                      aaaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGtt                                           <  2:4832159/101‑1 (MQ=255)
                                                       aaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCATCCGTTg                                          <  1:4494222/101‑1 (MQ=255)
                                                        aaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGc                                         <  2:5005351/101‑1 (MQ=255)
                                                        aaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGAGTGGCGAAAATTTCGATTTCGCAACCGTTGc                                         <  1:2602154/101‑1 (MQ=255)
                                                           atCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgc                                      >  2:6183321/1‑101 (MQ=255)
                                                            tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgcc                                     <  1:5881711/101‑1 (MQ=255)
                                                               gCATGACGCGGTGAATGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgcc                                  >  2:6883659/1‑101 (MQ=255)
                                                               gCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgcc                                  >  2:1207256/1‑101 (MQ=255)
                                                               gCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgcc                                  <  1:6291791/101‑1 (MQ=255)
                                                               gCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgcc                                  <  1:851094/101‑1 (MQ=255)
                                                                cATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGccgccg                                 >  1:5561185/1‑101 (MQ=255)
                                                                 aTGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCcgccgc                                >  1:3196406/1‑101 (MQ=255)
                                                                 aTGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCcgccgc                                >  2:4154282/1‑101 (MQ=255)
                                                                  tGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCa                               <  1:2457076/101‑1 (MQ=255)
                                                                   gACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAg                              >  1:377961/1‑101 (MQ=255)
                                                                      gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGGGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                           >  2:3663428/1‑101 (MQ=255)
                                                                      gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                           <  1:2326596/101‑1 (MQ=255)
                                                                      gcgGTGAAGGAACGACTTTAATGACGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                           <  2:5839288/101‑1 (MQ=255)
                                                                        ggTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAc                         >  2:5243867/1‑101 (MQ=255)
                                                                        ggTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAc                         >  1:1694686/1‑101 (MQ=255)
                                                                        ggTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAc                         <  2:395854/101‑1 (MQ=255)
                                                                         gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGGTTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                        >  2:259056/1‑101 (MQ=255)
                                                                         gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                        >  1:4895727/1‑101 (MQ=255)
                                                                         gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                        >  1:6032161/1‑101 (MQ=255)
                                                                            aaGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCgg                     >  1:3359983/1‑101 (MQ=255)
                                                                              ggAACGACTTTAATGCCGAATCTTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGCCTACGCggga                   >  2:4558830/1‑99 (MQ=255)
                                                                              ggAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGGGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTa                   <  2:1481243/101‑1 (MQ=255)
                                                                              ggAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTa                   >  2:1330066/1‑101 (MQ=255)
                                                                                aaCGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTaaa                 >  1:4628309/1‑101 (MQ=255)
                                                                                aaCGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGGGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGCaaa                 >  1:6313973/1‑101 (MQ=255)
                                                                                 aCGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAAc                <  1:4154282/101‑1 (MQ=255)
                                                                                  cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa               <  2:2813301/101‑1 (MQ=255)
                                                                                  cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa               <  1:5148391/101‑1 (MQ=255)
                                                                                    ccTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa             <  2:1647887/100‑1 (MQ=255)
                                                                                    aCTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa             >  1:394023/1‑101 (MQ=255)
                                                                                     cTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAg            >  1:1542406/1‑101 (MQ=255)
                                                                                      tttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAgg           <  1:5749736/101‑1 (MQ=255)
                                                                                      tttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAgg           >  2:4290675/1‑101 (MQ=255)
                                                                                       ttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGc          <  1:3034283/101‑1 (MQ=255)
                                                                                        tAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCa         >  1:3450700/1‑101 (MQ=255)
                                                                                        tAATGCCGAATCCTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCCACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCa         <  2:340636/101‑1 (MQ=255)
                                                                                         aaTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAg        >  2:2853380/1‑101 (MQ=255)
                                                                                          aTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGa       >  2:3064853/1‑101 (MQ=255)
                                                                                           tGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGaa      <  1:687842/101‑1 (MQ=255)
                                                                                            gCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAAc     <  1:2724247/101‑1 (MQ=255)
                                                                                            gCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAAc     <  1:6528870/101‑1 (MQ=255)
                                                                                             ccGAATCGTTCTGCATCAAACAGCGGGGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACg    <  2:5410746/101‑1 (MQ=255)
                                                                                              cGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACGt   >  2:2038222/1‑101 (MQ=255)
                                                                                               gAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACGtt  <  1:1278963/101‑1 (MQ=255)
                                                                                               gAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACGtt  <  1:4642643/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACGTT  >  NC_000913/2841934‑2842129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑