BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	704,236	T→C	L357S (TTG→TCG)	nagE →	fused N‑acetyl glucosamine specific PTS enzyme: IIC, IIB, and IIA components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	704,236	0	T	C	100.0%	1122.1 / NA	262	L357S (TTG→TCG)	nagE	fused N‑acetyl glucosamine specific PTS enzyme: IIC, IIB, and IIA components
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (142/120); total (142/120)

Only 100 of 278 total aligned reads displayed.

TACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTG  >  NC_000913/704136‑704333

    ggTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                               <  1:6496169/100‑1 (MQ=255)

    cgTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                               >  2:2275239/1‑101 (MQ=255)

    cattGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                               >  1:3473337/3‑101 (MQ=255)

     gTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTa                                                                                              <  2:1440342/101‑1 (MQ=255)

      ttGATGGATAACCTGCCGGCCGCCAGCCAGAACGCCTGGATGCTGCTGGAGATGGGCGTTATCTTCTTCGCTATCTACTTCGAGGTGTTCAGTTCGGTTAt                                                                                             >  2:5298416/1‑101 (MQ=255)

        gATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATcc                                                                                           >  2:3696543/1‑101 (MQ=255)

          tGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGc                                                                                         >  1:3296356/1‑101 (MQ=255)

            tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCan                                                                                       >  2:6958245/1‑100 (MQ=255)

             ataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATg                                                                                      >  1:3305406/1‑101 (MQ=255)

              taACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGt                                                                                     <  1:1167567/101‑1 (MQ=255)

               aaCCTGCCGGCCGCCAGCCAGAACGTCTGGGTGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACCTCGTGGTGTTCAGTTCGGTTATCCGCATGtt                                                                                    >  2:3801175/1‑101 (MQ=255)

                 ccTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCa                                                                                  <  2:4310718/101‑1 (MQ=255)

                  cTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCaa                                                                                 >  1:6456334/1‑101 (MQ=255)

                   tGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAc                                                                                <  1:5226180/101‑1 (MQ=255)

                   tGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAc                                                                                <  2:2823520/101‑1 (MQ=255)

                    gccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAcc                                                                               >  2:2309982/1‑101 (MQ=255)

                     ccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCt                                                                              <  1:3920576/101‑1 (MQ=255)

                       ggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGa                                                                            >  1:6965541/1‑101 (MQ=255)

                       ggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGa                                                                            <  2:246517/101‑1 (MQ=255)

                        gccgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                           >  1:3684575/1‑101 (MQ=255)

                         ccgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaa                                                                          >  2:1467912/1‑101 (MQ=255)

                           gccagccaGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAc                                                                        <  1:780309/101‑1 (MQ=255)

                           gccagccaGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAc                                                                        <  1:4613678/101‑1 (MQ=255)

                              agccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGTTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGcc                                                                     <  1:5404918/101‑1 (MQ=255)

                              agccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGcc                                                                     <  1:1341084/101‑1 (MQ=255)

                               gccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTCTCCGCATGTTCAACCTGAAAACGCCg                                                                    <  1:6860077/101‑1 (MQ=255)

                               gccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGAATGTTCAACCTGAAAACGCCg                                                                    >  2:6197753/1‑101 (MQ=255)

                                 cagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCggg                                                                  >  1:6207961/1‑101 (MQ=255)

                                  agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCgggg                                                                 >  2:6659922/1‑100 (MQ=255)

                                  agAACGTCTGGATGCTGCTGGTGATGGGCGTTAGCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGt                                                                 >  2:3459612/1‑101 (MQ=255)

                                   gAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTc                                                                <  1:2758268/101‑1 (MQ=255)

                                   gAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTc                                                                <  2:1631889/101‑1 (MQ=255)

                                      cGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTg                                                             >  1:4506711/1‑101 (MQ=255)

                                       gTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGa                                                            >  1:4119311/1‑101 (MQ=255)

                                         cTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAg                                                          <  1:4333118/101‑1 (MQ=255)

                                         cTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAg                                                          >  1:997991/1‑101 (MQ=255)

                                          tGGATGCTGCTGGTGATGGGCGTTATCTTGTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAgt                                                         >  2:3474912/1‑100 (MQ=255)

                                          tGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGa                                                         >  2:2733247/1‑101 (MQ=255)

                                           ggATGCGGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGGGAAGAt                                                        <  1:199006/101‑1 (MQ=255)

                                            gATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCCTGTTCAACCTGAAAACGCCGGGTCGTGAAGATa                                                       <  2:3273122/101‑1 (MQ=255)

                                                  gctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                                 >  2:4704914/1‑101 (MQ=255)

                                                  gctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                                 <  2:1745349/101‑1 (MQ=255)

                                                  gctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                                 >  1:3548043/1‑101 (MQ=255)

                                                  gctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                                 >  2:3957994/1‑101 (MQ=255)

                                                    tgGTGATGGGCGTTATCTTCTTCGCTATCTTCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAc                                               <  1:6574226/101‑1 (MQ=255)

                                                    tgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAc                                               <  2:4290070/101‑1 (MQ=255)

                                                     ggTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACg                                              <  1:2514298/101‑1 (MQ=255)

                                                     ggTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACg                                              <  1:2246969/101‑1 (MQ=255)

                                                      gTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACga                                             <  1:3042331/101‑1 (MQ=255)

                                                      gTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACga                                             <  2:2021126/101‑1 (MQ=255)

                                                       tGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGGCGTGAAGATAAAGAAGACgag                                            >  2:459089/1‑101 (MQ=255)

                                                        gATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                           >  2:4163064/1‑101 (MQ=255)

                                                         aTGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAt                                          >  1:5122346/1‑101 (MQ=255)

                                                          tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATc                                         <  2:5591502/101‑1 (MQ=255)

                                                           gggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCg                                        >  1:5904413/1‑101 (MQ=255)

                                                             gCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGtt                                      >  2:33913/1‑101 (MQ=255)

                                                               gTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTAc                                    >  2:5029418/1‑101 (MQ=255)

                                                               gTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTAc                                    >  2:6425897/1‑101 (MQ=255)

                                                                ttctcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGCCGAGATCGTTACt                                   >  2:1807765/4‑101 (MQ=255)

                                                                 tATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTg                                  <  2:4019382/101‑1 (MQ=255)

                                                                 tATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTg                                  <  1:2608692/101‑1 (MQ=255)

                                                                 tATCTTCTTCGCTATCGACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTg                                  <  2:2045229/101‑1 (MQ=255)

                                                                  aTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGCTATCCGCATTTTCAACCTGAAAACGCAGGGTCGTGAAGATAAAAAAGAGGAGATCGTTACTga                                 <  2:6061682/101‑1 (MQ=255)

                                                                   tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                                <  2:4830827/101‑1 (MQ=255)

                                                                   tcttctTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                                >  1:5054547/1‑101 (MQ=255)

                                                                    cttcttCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaag                               >  1:6714618/1‑101 (MQ=255)

                                                                    cttcttCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaag                               <  1:6122558/101‑1 (MQ=255)

                                                                     ttcttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaaga                              <  2:817687/101‑1 (MQ=255)

                                                                     ttcttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaaga                              <  1:3238032/101‑1 (MQ=255)

                                                                      tcttcGCTATCTACTTCGTGGGGTTCAGTTCGGTTATCCGCAGGTTCAACCTGAAAACGCCGGGGCGTGAAGATAAAGAATACAAGATCTTCACTggacaa                             >  2:2602607/1‑96 (MQ=255)

                                                                       cttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGaagaag                            <  1:3838562/101‑1 (MQ=255)

                                                                        ttcGCTATCTTCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGc                           <  1:2321234/101‑1 (MQ=255)

                                                                        ttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGc                           <  1:3142789/101‑1 (MQ=255)

                                                                        ttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGc                           <  1:5836939/101‑1 (MQ=255)

                                                                           gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCaa                        >  2:2965065/1‑101 (MQ=255)

                                                                           gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCaa                        >  1:1515450/1‑101 (MQ=255)

                                                                            cTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc                       >  2:6512808/1‑101 (MQ=255)

                                                                            cTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc                       >  1:1033753/1‑101 (MQ=255)

                                                                            cTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc                       >  1:2798656/1‑101 (MQ=255)

                                                                            cTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc                       >  1:5440885/1‑101 (MQ=255)

                                                                             tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACa                      <  1:5485915/101‑1 (MQ=255)

                                                                              aTCTACTTCGTAGTGTTCAGTTCGGTTATCCGCATGTTCAAACTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATAGTTACTGAAGAAGCCAACAg                     >  2:6310601/1‑101 (MQ=255)

                                                                               tCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGc                    >  1:1445064/1‑101 (MQ=255)

                                                                                  tcTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAac                 <  1:4058033/100‑1 (MQ=255)

                                                                                   cTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca                >  1:1673936/1‑101 (MQ=255)

                                                                                   cTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca                <  2:4214069/101‑1 (MQ=255)

                                                                                     tCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACt              <  2:1193054/101‑1 (MQ=255)

                                                                                     tCGGGGTGTTCAGTTCGGTTACCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACt              <  2:6587939/101‑1 (MQ=255)

                                                                                      cGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTg             >  2:4527769/1‑101 (MQ=255)

                                                                                        tggcgTTCCGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgat           >  2:2162047/1‑100 (MQ=255)

                                                                                          gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAGCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaaga         <  1:1022853/101‑1 (MQ=255)

                                                                                           tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaagaa        <  1:4964698/101‑1 (MQ=255)

                                                                                            gtTCAGTTCGGTTATCCGCATGTTCAACCTGGAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTGACTGAAGAGGCCAACAGCAACACTGaagaag       >  2:3117028/1‑101 (MQ=255)

                                                                                            gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGgagaag       >  1:4749934/1‑101 (MQ=255)

                                                                                             ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAgg      <  1:1593320/101‑1 (MQ=255)

                                                                                                tgTTCGGTTATCCGCATGTTCACCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCt   <  2:5788858/100‑1 (MQ=255)

                                                                                                aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCTTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCt   >  2:3317279/1‑101 (MQ=255)

                                                                                                aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTTAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCt   <  2:2791400/101‑1 (MQ=255)

                                                                                                aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGGTACTGAAGAAGCCAACAGCAACACTGAAAAAGGTCt   >  2:6008711/1‑101 (MQ=255)

                                                                                                 gTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTg  <  2:5122542/101‑1 (MQ=255)

TACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTG  >  NC_000913/704136‑704333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`