New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3461875153 (1.380)5 (0.040)
+TTG		 3/454 NT 3.2% coding (408/510 nt) gspH Type II secretion system protein GspH
?NC_000913 3461876 = 154 (1.350)coding (409/510 nt) gspH Type II secretion system protein GspH
Rejected: Frequency below/above cutoff threshold.

GGCGCGGTTTACCGCTGCACTTGAATTAGCCATCGACCGCGCCACGCTCAGTGGTCAGCCTGTCGGTATCCATTTCTCTGATTCAGCATGGCGCATTATGGTACCGGGCAAAACACCATCAGCCTGGCGCTGGGTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATCACGCCCTTCTCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3461599‑3461875
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGTATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTT  >  NC_000913/3461876‑3462135
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                    
GGCGCGGTTTACCGCTGCACTTGAATTAGCCATCGACCGCGCCACGCTCAGTGGTCAGCCTGTCGGTATCCATTTCTCTGATTCAGCATGGCGCATTATGGTACCGGGCAAAACACCATCAGCCTGGCGCTGGGTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATCACGCCCCTCTCTTTGT                                                                                                                                                                                                                                                                     >  1:798665/1‑281
  CGCGGTTTACCGCTGCACTTGAATTAGCCATCGACCGCGCCACGCTCAGTGGTCAGCCTGTCGGTATCCATTTCTCTGATTCAGCATGGCGCATTATGGTACCGGGCAAAACACCATCAGCCTGGCGCTGGGTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATCACGCCCTTCTCTTTGTTG                                                                                                                                                                                                                                                                   <  2:1166452/281‑1
                                                                                                                                                         CGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATCACGCCCTTCTCTTTGTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGT                                                                                                                                                                                                                       <  1:1181423/174‑1
                                                                                                                                                         CGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATCACGCCCTTCTCTTTGTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGT                                                                                                                                                                                                                       >  2:1181423/1‑174
                                                                                                                                                                                                                                                                GGACAAATCACGCCCTT‑TCTTT‑TT‑TTGATGGCTAACGCCGGGACCGGTGTGCCGCTCCTGACGTTGGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAGCAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGTATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTT  <  2:649211/281‑1
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                    
GGCGCGGTTTACCGCTGCACTTGAATTAGCCATCGACCGCGCCACGCTCAGTGGTCAGCCTGTCGGTATCCATTTCTCTGATTCAGCATGGCGCATTATGGTACCGGGCAAAACACCATCAGCCTGGCGCTGGGTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATCACGCCCTTCTCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3461599‑3461875
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGTATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTT  >  NC_000913/3461876‑3462135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.