BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,529,203	C→A	8.6%	pseudogene (1314/2049 nt)	rhsE →	RhsE protein in rhs element

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,529,203	0	C	A	8.6%	336.2 / 14.6	118	pseudogene (1314/2049 nt)	rhsE	RhsE protein in rhs element
Reads supporting (aligned to +/- strand): ref base C (44/62); new base A (5/6); total (49/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 118 total aligned reads displayed.

GAGACAGAGAACGGCGAGCGGGAAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGA  >  NC_000913/1528925‑1529471

gagaCAGAGAACGGCGCACGGGGAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCt                                                                                                                                                                                                                                                                              <  1:796993/281‑1 (MQ=21)

        gaACGGCGAGCGGGAAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTgg                                                                                                                                                                                                                                                                      >  1:387447/1‑281 (MQ=34)

                aGCGGGAAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCtcatc                                                                                                                                                                                                                                                              >  1:385005/1‑281 (MQ=35)

                 gCGGGAAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGCCGGTGGAGCACCTGGCCAGCCAGGTGGAGCCGGAATACACACCGGGGCGAAAAGGTCATTTTTACCACCGCGACCACCGGGGCCTGCCGCTGgcgcgcccca                                                                                                                                                                                                                                                             >  2:684241/1‑275 (MQ=255)

                       aaaaGCGCAGCGGCGCAGCCTTGCAGAGCGACTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCTGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGaa                                                                                                                                                                                                                                                        <  1:173023/280‑1 (MQ=255)

                       aaaaGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGaa                                                                                                                                                                                                                                                        <  1:181241/280‑1 (MQ=35)

                                gcggcgCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAGATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATa                                                                                                                                                                                                                                              <  1:166559/281‑1 (MQ=25)

                                           tGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACAAACCGGCGCGAAAAGTTCATTTTTACCACTGCGCCCACCGGGGCCTGCCGCTGGCGCTAATCAGCGCAGACGGCAATACGGCggggggg                                                                                                                                                                                                                                   >  2:860963/1‑278 (MQ=255)

                                            tcccaggaCGCTCCGGCAAGAAGGGGGTGGGAGCAGCCACGGCGTGGTGTTCCCGGCTGAACTGTTGCGGCTGCGTGACAGGCTGGAGGGAGAGATCCGAGCAGACGGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGGTCAGTGCGGGCTGGCGGTGGGGCAACTGGCCAGACGGGTGGAGCCGAAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGcgcg                                                                                                                                                                                                                                  <  2:567191/275‑1 (MQ=255)

                                             gCAGAGACGCTCCGGCAGGAAGGGAGTGATAACGGCAACGGGGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAAACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGcgcg                                                                                                                                                                                                                                  <  1:1198650/280‑1 (MQ=255)

                                             gCAGAGACGCTCCGGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGcgcg                                                                                                                                                                                                                                  <  1:357064/280‑1 (MQ=37)

                                             gCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGcgcg                                                                                                                                                                                                                                  <  1:684241/280‑1 (MQ=37)

                                                   aCGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGACACCACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATCCGGCGTGGCGCGGGGAGTa                                                                                                                                                                                                                           >  1:133877/1‑281 (MQ=255)

                                                      cTCCAGCAGGAAGGGAGGGGGAACGGCCCAGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACAAACCTGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatg                                                                                                                                                                                                                         <  1:298206/280‑1 (MQ=255)

                                                          agcagGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTAtgatga                                                                                                                                                                                                                     <  2:115453/280‑1 (MQ=39)

                                                                 aGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGTGTGAGCAGGGAAAGTCGGGCGTGGCCTGTGCAGTGCGGGCTGACGGTGGAGCAACTTGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGAAATAAGGCGTGGCGCGGGGGGTAGGGTGAATGTGGCa                                                                                                                                                                                                             >  1:921401/1‑281 (MQ=14)

                                                                 aGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGCCGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCa                                                                                                                                                                                                             >  1:563247/1‑281 (MQ=39)

                                                                 aGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGTGCgg                                                                                                                                                                                                                                 >  1:641544/1‑261 (MQ=37)

                                                                     aGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCaa                                                                                                                                                                                                            <  1:568314/278‑1 (MQ=39)

                                                                            aCGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTaa                                                                                                                                                                                                   <  1:860963/280‑1 (MQ=39)

                                                                               gCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCCTCAGCGAAGACGGCAATACGGCGTGGCGCGGGGGGGATGATGAATGGGGCAACCAGGTTAATg‑ag                                                                                                                                                                                              >  1:935058/1‑281 (MQ=255)

                                                                                ccACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATg‑ag                                                                                                                                                                                              <  1:1054911/280‑1 (MQ=39)

                                                                                         ggtgTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGc                                                                                                                                                                                    <  2:387447/281‑1 (MQ=39)

                                                                                               cccGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCAc                                                                                                                                                                               <  1:879122/280‑1 (MQ=40)

                                                                                                ccGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGGGGCGCGGGGAGTATGATGAATGGGGCAACCCGCCTATTG‑TGGAGAAACCGCAGCAcc                                                                                                                                                                              >  1:1223416/1‑280 (MQ=255)

                                                                                                     tGAACTGGTTCGGCTGCTGGAAAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGTCGGGCGTGGCTTGCGCAGTGCGGGCAGACGGTGGAGCAACTGGCCAGCACGGTGGAGCCGGAATAAACACCGGCGCGAAAAGTTCATTGTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGAAGGCAATACGGCGTGGGGGGGGGAGTATGATGAATGGGGGAAACAGCCTAATG‑CGGAGAACCCGCagtccctcacc                                                                                                                                                                         >  2:288604/1‑270 (MQ=11)

                                                                                                         cTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGGTGAATGGGGCCACCAGGTTAATG‑GGGAGAACCAGCATCACCCGCACCacc                                                                                                                                                                     >  2:29103/1‑278 (MQ=255)

                                                                                                                      tgGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGgcg                                                                                                                                                                                                                                                                    <  2:759392/173‑1 (MQ=38)

                                                                                                                      tgGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGgcg                                                                                                                                                                                                                                                                    >  1:759392/1‑173 (MQ=38)

                                                                                                                          cAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAggg                                                                                                                                                    <  1:492204/280‑1 (MQ=255)

                                                                                                                             gCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCCATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAAGCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGgc                                                                                                                                                   >  1:878565/1‑278 (MQ=255)

                                                                                                                              cTGGGGGAAGAAATCCGGGCAGACCGCGTGGGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGgcagca                                                                                                                                               <  1:29103/281‑1 (MQ=255)

                                                                                                                              cTGGGGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGgcagca                                                                                                                                               <  1:490616/281‑1 (MQ=12)

                                                                                                                              atGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCAAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGgcagca                                                                                                                                               <  1:288604/280‑1 (MQ=255)

                                                                                                                                       gaaATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                                                                                                      >  1:680954/1‑281 (MQ=255)

                                                                                                                                             cggggcgcccGCGGGAGCTGTGAAAGCCGGGCGTGGGTGGGCAAGGGGGGGCTGACGGTGGGGCAACGGGCCAGACGGGTGGGGCCGAAATACGCACCGGCGAGAAAGGTTTATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATGAGCGAAGACGGCAATCCGGCGTGGCGCGGGGAGGATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGt                                                                                                                                 <  2:935058/272‑1 (MQ=14)

                                                                                                                                             cggggcgACCGCGGGAGCAGTGAAAGCCGGGCGTGGCGGCGGCAGTGCGGGCTGGCGGTGGAGCAACTGGCCAGAAAGGGGGAGCCGGAATACACGCCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGGCGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGt                                                                                                                                 <  2:196788/274‑1 (MQ=11)

                                                                                                                                             cGGGCAGACCGGATGGGCACTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCCGACGGTGGAGCAACTGGCCAGACAGGTGTAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGt                                                                                                                                 <  1:200677/280‑1 (MQ=255)

                                                                                                                                             cGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCGTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGt                                                                                                                                 <  1:79909/280‑1 (MQ=255)

                                                                                                                                                 cAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCgggc                                                                                                                            <  1:802371/281‑2 (MQ=255)

                                                                                                                                                  aGACCGGGGGTGCAGGAAAAGGCCGGGGGGGCTTGCGCAGTTGGGGCTGACGGGTGAGCAATGGGCCAGACAGGTGGGGCCGGAATACACACCGGCGCGAAAAGTTCATTGTTACCACTGCGACCACCGGGGCGTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCgggg                                                                                                                            <  2:588140/280‑1 (MQ=14)

                                                                                                                                                  aGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGc                                                                                                                           <  1:208162/281‑1 (MQ=255)

                                                                                                                                                  aGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGc                                                                                                                           <  1:929395/281‑1 (MQ=255)

                                                                                                                                                    aCCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTAGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCCTATCAGCGAAGACGGCAATACGGCGTGGCGGGGGGAGGACGACTAATGGGGCCACCACCATAATG‑AGGGGACACCGCATCACCTGCCCCAACAGTACCCTCTGCCAGGGAACAAGCACGCTGAGGATGCgggggcg                                                                                                                         >  2:679855/1‑278 (MQ=11)

                                                                                                                                                                    acagcGGGCGTGGCTTGCGCAGTGCGGGGTGACGGTGCAGCCACTGGCCAGGCCGGGGGGCCCGGAATCCACACCGGGGCGAAAAGTTCATTTTTACCACGTAGTCCCCCGGGGCCTGCCGCTGGAGCTCATCAGCGGAGACGGCAATCCGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCAc                                                                                                         <  2:342793/276‑1 (MQ=14)

                                                                                                                                                                       ccGGGCGTGGCTGGGGGAGTCCGGGCCGAGGGTGGGTAAACTGTCCAGTCAGGTGGGAGCGGACTACGCACCGGCGCGAAAAATTCATTTTTACCGCTGCGGCCGCCGGGGCCGGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGGGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCgg                                                                                                      <  2:911087/280‑1 (MQ=14)

                                                                                                                                                                           gCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGc                                                                                                                                                                           >  1:263433/1‑208 (MQ=255)

                                                                                                                                                                           gCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGc                                                                                                                                                                           <  2:263433/208‑1 (MQ=255)

                                                                                                                                                                           gCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑CACCGTCACCGGCACTa                                                                                                 >  1:487228/1‑281 (MQ=255)

                                                                                                                                                                                gCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGACTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCt                                                                                                                          >  1:364617/1‑252 (MQ=255)

                                                                                                                                                                                gCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCGGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGTGCGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTTACGTCTGCCAGGGCAGAAGCATGCTGAGGGGTCGGGCCGGGACGAT‑AACAGTACCCCGCCCTAGGATc                                                                                            >  2:187809/1‑281 (MQ=14)

                                                                                                                                                                                 ggttcggagggcgggcggGGGGTGGAGCCACGTGCCGGACGGTGGGAGCCGCAATACACCCCGGGTCGAAAAGTTAATTTTTGCCCCGGCTGCCGCCGGGGCACGCCGCTGGCGCCCATCCGCCAAGACGGCAATACGGAGTGGCGCGGGGAGTATGATGAAGGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGGTGAGGGGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATc                                                                                            <  2:1223416/270‑1 (MQ=17)

                                                                                                                                                                                           gcgggcggACGGTGGAGCAACTGGCCAGACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAgggg                                                                                 <  1:478910/281‑1 (MQ=255)

                                                                                                                                                                                           gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAgggg                                                                                 <  1:311227/281‑1 (MQ=255)

                                                                                                                                                                                           gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTGCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAgggg                                                                                 <  1:654045/281‑1 (MQ=255)

                                                                                                                                                                                               gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGTGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGAGGAATGGGGCAACCAGCTTAATGAAGGAGAACACCAAGCAACTGACACAGACGTACCGTCTGCTCGGGCAGCAGCATGATTAGGAGAAGGGGCCGTCCTTTAAACCGT‑AACGGCACCATGATTCGTTCGAGGGGcc                                                                               >  2:576499/1‑279 (MQ=14)

                                                                                                                                                                                                    cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGCCCGACGGGGCCAGCCGCTGGCGCGCCACAGCGAAGACGGCCATACGGCGTGGCGCGGGGAGTGTGATGGCTAGGGAAACCAGCTTAATG‑AGGAGAACCCGGATTACCTGCATCGACCGTACCGTCTGCCAGTGCCGCAACATGACGAGGGGTTGGGTGTGTGCTAT‑AGGCGTGACCGGCACCACTATCCGTGGCAGGGGCGGgatga                                                                         >  2:458482/1‑278 (MQ=14)

                                                                                                                                                                                                    cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTACTG‑AGGAGAACCGGCATCACCTGCACCAGCCGTACGGTCAGCCAGGGGAGCAGCAGGATGAGGAGTCGGGGCTGTACTCT‑AACCGTCACCGGCACTACCCACCGTTGCAGGGGCGGtagag                                                                         >  2:59492/1‑277 (MQ=17)

                                                                                                                                                                                                    cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTg                                                                                                                         <  1:696775/233‑1 (MQ=255)

                                                                                                                                                                                                    cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTg                                                                                                                         >  2:696775/1‑233 (MQ=255)

                                                                                                                                                                                                               cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGCTGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGAccc                                                             >  1:838260/1‑281 (MQ=255)

                                                                                                                                                                                                               cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGAccc                                                             >  1:862717/1‑281 (MQ=255)

                                                                                                                                                                                                                 ggCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTg                                                                                                                         >  2:31849/1‑220 (MQ=255)

                                                                                                                                                                                                                 ggCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTg                                                                                                                         <  1:31849/220‑1 (MQ=255)

                                                                                                                                                                                                                 ggCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCCCCCCGGACCCGa                                                           >  1:1028336/1‑281 (MQ=255)

                                                                                                                                                                                                                 ggCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGCGTATGATGAATGGGGCAACCAGCTTAAGG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGAAGCATGACGAGGAGTCGGGGCTGTACGAT‑AACCGTCACCGGCACTACGATCGGTTGAAGGGGGGGTATACAACCCCGGACCCGa                                                           >  2:275907/1‑281 (MQ=11)

                                                                                                                                                                                                                           ggggACCCGGAATACACCCCGGCGAGAAAAGTTCATTTTTGCCACTGCGACCACCGGGGCCTGCCGCTGGGGCTAATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGACCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTga                                                  <  2:359410/278‑1 (MQ=255)

                                                                                                                                                                                                                             ggAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑ACCCGTCACCGGCACCACGCTCCGTTGGAGGGGCGGGATACCACCCCGGACCCGACGGGGTTgggcg                                               >  2:463490/1‑277 (MQ=255)

                                                                                                                                                                                                                                 ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCat                                                                                                                                           >  1:866763/1‑186 (MQ=255)

                                                                                                                                                                                                                                   ggaaaagccacCGGCGCGGAGATTTCATTTTTTGCCCTGCGACCACCGGGGCCTACCGCTGGCGCTCAACAGGGAGGACGGGAAGACGGCGTGGCGCGGGGAGTATGATAAATGAGGAAACCGGCTTAATG‑AGGGGAACCCGCATCACCTGCACCAGCCATACCGTCTGCCAGGGAAGCGGCATGATGGGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGGACTGCGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGtgga                                          <  2:731742/272‑1 (MQ=255)

                                                                                                                                                                                                                                   ggAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGAAGCAGCATGATGAGGAGTCGGGGCGGTACTAT‑AACCGTCACCGGCCCTACGATCCGTTGCAGGGGCGGTGTATCACCCCGGACCCGAGTGGGTTGAGAGGgggag                                         >  2:729118/1‑280 (MQ=255)

                                                                                                                                                                                                                                    gaaacaacacCGGCGGGAAGAGATCATTTTTACCACTGCGACCGCCGGGGCCGCCCGCAGGCGCTCACCAGCGAGGACGGAAAAGCGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCAGCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGAGCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGtggatg                                        <  2:239721/275‑1 (MQ=17)

                                                                                                                                                                                                                                    gAATACCCACCGGCGCGAATAGTTCATTTTTACCACTGCGACCACCAGGGCCTGCCGCTGGAGCTCATCAGCGAAGACGGAAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAAGTAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGtgga                                          <  1:576499/280‑1 (MQ=255)

                                                                                                                                                                                                                                    gAATACACCCCGGCGGGAAAAGTTCATTTTTACCCCTGCGACCACCGGGGCCTGCCGATGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGt                                             <  1:187809/276‑1 (MQ=255)

                                                                                                                                                                                                                                     aaTACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTGGAGAGGtggatgg                                       >  1:140850/1‑281 (MQ=255)

                                                                                                                                                                                                                                      atccccccaggCGCGAAAAGTTCATTGTTTCCACTGCGCCCCCCGGGGCCGGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGGATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGtggatgga                                      <  2:174644/272‑1 (MQ=255)

                                                                                                                                                                                                                                      aTACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACg                                                                                               >  1:500254/1‑224 (MQ=255)

                                                                                                                                                                                                                                      aTACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACg                                                                                               <  2:500254/224‑1 (MQ=255)

                                                                                                                                                                                                                                       tacaacgcggcggAAAAGGCTCGTTTTTATCCCGGCGCCCACCGGGGAGTGCGCCGGGGGCTTATGAGGGAAGAGGGCAAAACGGCGGGGGGCGGGGAGTATGAGGAATGGGGCAACCAGCTTAAAG‑AGGAGAACCCGCATCACGTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGtggatgga                                      <  2:487228/273‑1 (MQ=14)

                                                                                                                                                                                                                                       tACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGtggatgga                                      <  1:135387/280‑1 (MQ=255)

                                                                                                                                                                                                                                       gacaacccGGCGCTATAAGTTCATTTTTTCCACTTCTACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATTGGGCAACCAGCTTAATG‑GGGAGAACCCGCGTCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGGTCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGtggatgga                                      <  1:1112531/274‑1 (MQ=17)

                                                                                                                                                                                                                                              cGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCg                                                                                                                                                                   >  2:961843/1‑149 (MQ=255)

                                                                                                                                                                                                                                              cGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCg                                                                                                                                                                   <  1:961843/149‑1 (MQ=255)

                                                                                                                                                                                                                                              cGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                                                                                                        >  2:125413/1‑176 (MQ=255)

                                                                                                                                                                                                                                              cGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGCGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGCGAGGtggatgga                                      >  1:239721/1‑273 (MQ=255)

                                                                                                                                                                                                                                              cGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTAt                              <  1:518348/281‑1 (MQ=255)

                                                                                                                                                                                                                                               ggggctaaaaGTTCATTTTTACCACAGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑GGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTACCCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATc                             <  1:1156845/275‑1 (MQ=255)

                                                                                                                                                                                                                                                 cgcgCAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCa                                                                                                                                                       >  1:947543/1‑158 (MQ=255)

                                                                                                                                                                                                                                                 cgcgAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGGGGTGGATGGAATATGttacag                           >  1:784062/1‑276 (MQ=255)

                                                                                                                                                                                                                                                 cgcgAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCa                                                                                                                                                       <  2:947543/158‑1 (MQ=255)

                                                                                                                                                                                                                                                 cgcgAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGgcagca                                                                                                                                               >  2:137228/1‑166 (MQ=255)

                                                                                                                                                                                                                                                 cgcgAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGgcagca                                                                                                                                               <  1:137228/166‑1 (MQ=255)

                                                                                                                                                                                                                                                 cgcgAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCCCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGATTAATG‑AGGAGAACCCGCATCTCCTGCACCAGCTGTACCGTCTGCCAGGGCAGTAGCATGATGCGGAGTCGGGGCTGTATTAT‑AACGGTCAACGGCACTAAGATCCGTGGCAGGGGCGGTATATCACCACGGAACCGATTGGGTGGGGGGGTGGATGGAatattgttaag                           >  2:1205360/1‑274 (MQ=255)

                                                                                                                                                                                                                                                                   aCCACTGCGACCACCGTGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCA‑CCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATAc        <  1:458482/281‑1 (MQ=255)

                                                                                                                                                                                                                                                                    ccACTGCGACCACCGGGGCCTGACGCTGGCGCGTATGAGCGAAGACGGCAATACGGCGTGGCGCGGGGGGTATGATGAATGGTGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCCCCCCGGACCAGATTGGGTTGAGAGTTAGATGGAATATGTATCAGTATCCGTTGAATCCCATAc        <  1:971023/281‑1 (MQ=18)

                                                                                                                                                                                                                                                                     gccggCGACCACCGGGGACTGCCGCTGGGGCTTATCAGGGAGGACGGCAAGACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACa       <  1:679855/277‑1 (MQ=255)

                                                                                                                                                                                                                                                                          ggACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGa  <  1:544305/280‑1 (MQ=255)

                                                                                                                                                                                                                                                                            accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGaa                                                                                                                                                                                         <  2:603829/97‑1 (MQ=16)

                                                                                                                                                                                                                                                                            accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGaa                                                                                                                                                                                         >  1:603829/1‑97 (MQ=16)

                                                                                                                                                                                                                                                                                  ggggCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGgcagca                                                                                                                                               <  2:612592/133‑1 (MQ=255)

GAGACAGAGAACGGCGAGCGGGAAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATG‑AGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTAT‑AACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGA  >  NC_000913/1528925‑1529471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`