Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC39.5% 42.4 / 19.8 43intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base T (9/14);  new base C (13/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 6.45e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTT  >  NC_000913/4295989‑4296548
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                              
gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTCCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAcgccgc                                                                                                                                                                                                                                                                                           >  1:522804/1‑285 (MQ=18)
     cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                                                                                                                                                                       >  1:668860/1‑284 (MQ=25)
      gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAAGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGACTTAGCGGGATTTTGTAGGTCGGATACGGCGTTTCCGCCGCCTCCGa                                                                                                                                                                                                                                                                                     >  1:44669/1‑285 (MQ=255)
        aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTTATGCCGCATTCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCCCACAGCGCAATAGCCTTATTCAGCGTGATTTGGTAGGTCGGGTAAGGCGTCTCCGCCGCATCCGAcc                                                                                                                                                                                                                                                                                   >  2:444634/1‑284 (MQ=11)
          tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAGCGCGTCTTATCAGGCATACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAGGCCGTTTACGCCGCCTCCGAAAt                                                                                                                                                                                                                                                                                  >  1:911207/1‑284 (MQ=255)
               tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTCGCGTGATTTTGTCGGTCGGATCCGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTCTCAGGCCTACGCCCAACCGCGCCATAGCCTGCATTGTCTTGATCTTGGAGGTAGGACAAGGCGTTTACGCCCCactcacactcaatct                                                                                                                                                                                                                                                                            >  2:701741/1‑271 (MQ=11)
               tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTCTGTAGTTCGGTTACCGCCTTTACGCCGCATCCGCCATCact                                                                                                                                                                                                                                                                              >  2:214132/1‑281 (MQ=11)
                gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATGTTGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCAATGcc                                                                                                                                                                                                                                                                           >  1:352875/1‑285 (MQ=21)
                 aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTCACGCGTCTTAGCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTCCGGATCAGGCGTTTACGCCGCTTCCCActccattgcc                                                                                                                                                                                                                                                                           >  2:70027/1‑275 (MQ=11)
                      gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAAGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTCAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGTCGCATCCGACATCAATCCC‑TGATg                                                                                                                                                                                                                                                                     >  1:937192/1‑284 (MQ=16)
                                      cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCC‑TGATGCGACGCTTGCCGCGT‑c                                                                                                                                                                                                                                                    >  1:405520/1‑284 (MQ=17)
                                              gcagttccgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCC‑TGATGCGACGCTTGCCGCGT‑CTTATCAGGc                                                                                                                                                                                                                                           <  1:689000/278‑1 (MQ=9)
                                                   tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCCGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGCTTTTGTAGGTCGGTTAAGGCGTTTACGCCGCATCCGACATCAATGCC‑TGATGCGACGCTTGCCGCGT‑CTTATCAGGCCTat                                                                                                                                                                                                                                       >  1:890495/1‑283 (MQ=255)
                                                   tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCC‑TGATGCGACGCTTGCCGCGT‑CTTATCAGGCCTat                                                                                                                                                                                                                                       >  1:618024/1‑283 (MQ=17)
                                                   tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGAAATCAATGCC‑TGTTGCGACGCCTGCCGCGA‑CTTATCAGGCCTatc                                                                                                                                                                                                                                      >  2:166207/1‑283 (MQ=255)
                                                                                                                                                                                gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCCG‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt                                                                                                       >  2:114966/1‑285 (MQ=255)
                                                                                                                                                                                    tCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt                                                                                                   <  1:166207/285‑1 (MQ=255)
                                                                                                                                                                                             gctggcaCGCTTCGCGCGTCTTATCAGGCTTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                                                                          <  1:126780/279‑1 (MQ=11)
                                                                                                                                                                                                        tgccgcgTCTTCTCAGTCCTACGCCGGTCAGCTCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCAACCGACAGCCACGGC‑TGTTGCGACGCTTAACGCGG‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                <  1:560750/281‑1 (MQ=14)
                                                                                                                                                                                                        tgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                               <  1:40148/282‑1 (MQ=255)
                                                                                                                                                                                                        tgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                               <  1:777061/282‑1 (MQ=255)
                                                                                                                                                                                                                 taaccAGACCTCCGCAAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGCCCGCAGCCGACATCAACGCC‑TGATGCGCCGCTTAACGCGT‑CTTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                      <  1:804823/281‑1 (MQ=14)
                                                                                                                                                                                                                  acccAGGACGACGACAGACAGCGGAATGGCTGTATGTAGCGGGGTTGTGGAGGTCTAAGAAGGCGTGTACGCCGGCGCCGACATAAACGCCGTGA‑GCGACGCTGAGCGGGT‑CTTATCAGGCCTACTCCAGAAGGCGAAATAGCCTGATTTAGCGTGAGTTTGGAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                      <  2:99618/281‑1 (MQ=9)
                                                                                                                                                                                                                         ccTACGCCGGCAAGGGCAATAGCCTGAGTTAGCGGGATTTTGTAGGCGGGGTAAGGCGTTTAGTCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCGTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa                                                              <  2:404455/285‑1 (MQ=17)
                                                                                                                                                                                                                                cAGACATCGCAATAGCCTGATTTAGCGTGCTTTTGTATGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATTCTACGCTTAACGCGT‑CTGATCAGGCCTACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTGT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                       <  1:701741/285‑1 (MQ=17)
                                                                                                                                                                                                                                cAGACAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTTATGCCGCCTCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCATGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                       <  1:488429/285‑1 (MQ=255)
                                                                                                                                                                                                                                cAGAAAGGGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGGCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                       <  1:527318/285‑1 (MQ=255)
                                                                                                                                                                                                                                 aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                      <  1:641237/269‑1 (MQ=255)
                                                                                                                                                                                                                                     aGCACAATAGCCTGATTTTGCGTGATTTGGGAGGTCGGATAAGGCGTTTATGCCGCATCTGACATCAACGCC‑AGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAACGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                  <  1:476491/285‑1 (MQ=17)
                                                                                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGATTGCCGCGTCTTATCAGGCCTATCTTACCCGTCGGTTAATTTTTCAAACTCTTCACTCCCGGTATCAAACCCTTCCATACAGCTCAGGTTc                                                >  1:336187/1‑285 (MQ=255)
                                                                                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTACCGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGCTTTTGTAGGTCGGATAAGGCCTTT‑‑ACGCCGCATCCGACCTCAATGCCTGATGCGACCCTGGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTc                                                >  2:490334/1‑285 (MQ=17)
                                                                                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGtt                                                 >  1:462131/1‑284 (MQ=255)
                                                                                                                                                                                                                                       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGAGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCCCTTCACACCCGGACTCCACCCCTTc                                                                >  2:641237/1‑269 (MQ=14)
                                                                                                                                                                                                                                           aaagcttaagttAGCTTGATTGTGTAGGTCTGATAAGGCGTTTAGGCCGCATCCGACAGCACCGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCATACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                             <  1:399150/274‑1 (MQ=17)
                                                                                                                                                                                                                                            tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACATCTTCACACCCGGTATCAAACCCTTCCATACAGTTCAGGTTCAAcc                                            >  1:840695/1‑284 (MQ=255)
                                                                                                                                                                                                                                                 ggATTTGGCTGGAGTTTGTAGGTGGTATAAGGCGTTTAGGCCGCATCGGACATCAGCGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGGCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCTTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa                                      <  2:72789/284‑1 (MQ=18)
                                                                                                                                                                                                                                                   gtttttcgggagtctGTAGGGCTGATAAGACGTTTAGTCCCGATCCGAGATCAACGCC‑GCATGGGACGCTTAACGCGT‑CGTATCAGGCCTTCGCCAGACAGCTCAAGAGCCTGATTTTGCGTGATTTTTTAGTTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCGTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg                                     <  2:45379/270‑1 (MQ=18)
                                                                                                                                                                                                                                                        gctggATTTTGTAGTGCGGAAAAGCCGTATATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCTCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                <  1:182879/280‑1 (MQ=18)
                                                                                                                                                                                                                                                         cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGATTTAGCGTGCTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTATCTTAACCGTTGGTTAA‑TTTTCAACCCCTTCACACCCGGTATCAAACCCTTCCATACCGCTCAGGTCCAACCCGTTCAGCGCCtttt                             >  2:526748/1‑285 (MQ=17)
                                                                                                                                                                                                                                                             aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCt                          >  1:606540/1‑285 (MQ=255)
                                                                                                                                                                                                                                                             aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCt                          >  1:839503/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                     gtgagaacagggCGTTAATGCAGCATCCGAAATCAACGCC‑TGATGCGACGCTTAA‑GTGTGTTTGTCGTGCATACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttcttc                  <  2:234649/275‑1 (MQ=21)
                                                                                                                                                                                                                                                                                gCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAAcc       <  2:668860/285‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     tATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt  <  2:839503/285‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                              
GACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCC‑TGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTT  >  NC_000913/4295989‑4296548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑