Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,801,032	C→T	G323E (GGA→GAA)	waaR ←	lipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,801,032	0	C	T	100.0%	65.9 / NA	20	G323E (GGA→GAA)	waaR	lipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/10);  total (10/10)

TCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTG  >  NC_000913/3800886‑3801173                                                                                                                                                   |                                                                                                                                              tCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCt                                                                                                                                             >  5:61773/1‑149 (MQ=255)      tAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTgaga                                                                                                                                         <  6:61773/148‑1 (MQ=255)       aaTACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGat                                                                                                                                        <  4:49928/148‑1 (MQ=255)               gAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAatgat                                                                                                                                <  4:80636/148‑1 (MQ=255)                               ttttCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGt                                                                                                               >  2:15885/1‑149 (MQ=255)                                  tCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGat                                                                                                                                        <  8:142844/121‑1 (MQ=255)                                  tCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGat                                                                                                                                        >  7:142844/1‑121 (MQ=255)                                             aacaacCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCtttttt                                                                                                  >  6:107364/1‑148 (MQ=255)                                                           tGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCaataat                                                                                     <  5:107364/147‑1 (MQ=255)                                                                    tcttctGAATCATAATAAACCAGTTAAATGTTATTTACGATAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCAt                                                                          >  5:149050/1‑149 (MQ=255)                                                                                              aaTGTTATTTACGATAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCAACTTTCCAgggg                                                <  6:149050/149‑1 (MQ=255)                                                                                                tGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTc                                                             <  6:113273/134‑1 (MQ=255)                                                                                                tGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTc                                                             >  5:113273/1‑134 (MQ=255)                                                                                                     tttACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGttt                                                                                                             <  7:18521/81‑1 (MQ=255)                                                                                                     tttACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGttt                                                                                                             >  8:18521/1‑81 (MQ=255)                                                                                                     tttACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAAtttt                                         <  1:15885/149‑1 (MQ=255)                                                                                                            tAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATACCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGc                                  >  7:115675/1‑149 (MQ=255)                                                                                                                                      tCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCaa                                                                                         >  7:85242/1‑68 (MQ=255)                                                                                                                                      tCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCaa                                                                                         <  8:85242/68‑1 (MQ=255)                                                                                                                                            tataATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTg  >  7:85644/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                              TCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTG  >  NC_000913/3800886‑3801173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑