Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,287,443 G→T intergenic (‑72/‑321) acs ← / → nrfA acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,287,4430GT93.9% 100.2 / ‑1.0 33intergenic (‑72/‑321)acs/nrfAacetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (16/15);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

TGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAG  >  NC_000913/4287307‑4287576
                                                                                                                                        |                                                                                                                                     
tgAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTgg                                                                                                                           >  7:133259/1‑149 (MQ=255)
   gggTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGc                                                                                                                         >  1:149654/1‑148 (MQ=255)
       ttATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGa                                                                                                                       >  1:135922/1‑146 (MQ=255)
                     tCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAg                                                                                                        >  8:139492/1‑147 (MQ=255)
                                          gtgtgtTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACgg                                                                                   >  4:32636/1‑147 (MQ=255)
                                                   tgAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAAtt                                                                                                 <  8:16365/124‑1 (MQ=255)
                                                   tgAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAAtt                                                                                                 >  7:16365/1‑124 (MQ=255)
                                                           gCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGt                                                                <  1:56652/149‑1 (MQ=255)
                                                             tCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGt                                                                >  4:2972/1‑147 (MQ=255)
                                                                       gTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAg                                                    >  4:123392/1‑149 (MQ=255)
                                                                              ttGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGAAAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAtt                                              >  3:20886/1‑148 (MQ=255)
                                                                                 tAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAAtt                                          >  2:120541/1‑149 (MQ=255)
                                                                                    gATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCtt                                       <  8:133259/149‑1 (MQ=255)
                                                                                    gATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCtt                                       >  4:29662/1‑149 (MQ=255)
                                                                                        ttAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAg                                   <  3:123392/149‑1 (MQ=255)
                                                                                          aataatATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAgg                                  >  6:68092/1‑148 (MQ=255)
                                                                                           ataataTGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGa                                 <  7:139492/148‑1 (MQ=255)
                                                                                                   tgGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAg                         <  2:149654/148‑1 (MQ=255)
                                                                                                      cATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTat                     <  2:48257/149‑1 (MQ=255)
                                                                                                       aTAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAg                         >  3:130634/1‑144 (MQ=255)
                                                                                                       aTAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAg                         <  4:130634/144‑1 (MQ=255)
                                                                                                              ttAAATGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAAtt                                          <  6:21623/120‑1 (MQ=255)
                                                                                                                 aaTGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAAt                                                                                         <  5:39223/70‑1 (MQ=255)
                                                                                                                 aaTGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAAt                                                                                         >  6:39223/1‑70 (MQ=255)
                                                                                                                 aaTGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGtt           <  1:120541/148‑1 (MQ=255)
                                                                                                                 aaTGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGtt           <  2:135922/148‑1 (MQ=255)
                                                                                                                  aTGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                                      >  7:104061/1‑120 (MQ=255)
                                                                                                                  aTGTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                                      <  8:104061/120‑1 (MQ=255)
                                                                                                                    gTAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAtttt                                                                       >  4:72138/1‑85 (MQ=255)
                                                                                                                     tAGGGGTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGaaaa      <  3:29662/149‑1 (MQ=255)
                                                                                                                         ggTATTGGCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAagag  <  3:2972/149‑1 (MQ=255)
                                                                                                                                gCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                                      >  1:188387/1‑106 (MQ=255)
                                                                                                                                gCAGTTTTTTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                                      <  2:188387/106‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAG  >  NC_000913/4287307‑4287576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑