Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,654	G→T	intergenic (‑186/‑523)	gltA ← / → sdhC	citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,654	0	G	T	100.0%	94.9 / NA	28	intergenic (‑186/‑523)	gltA/sdhC	citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/13);  total (15/13)

ATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTC  >  NC_000913/754515‑754793                                                                                                                                            |                                                                                                                                            aTTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCa                                                                                                                                    <  1:3957/149‑1 (MQ=255)                       ggCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGaa                                                                                                                             <  6:107807/134‑1 (MQ=255)                       ggCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGaa                                                                                                                             >  5:107807/1‑134 (MQ=255)                               ccGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACtt                                                                                                                  <  6:121575/137‑1 (MQ=255)                               ccGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACtt                                                                                                                  >  5:121575/1‑137 (MQ=255)                               ccGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACtaa                                                                                                      >  7:2794/1‑149 (MQ=255)                               ccGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACtaa                                                                                                      >  7:23615/1‑149 (MQ=255)                                cGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACtaa                                                                                                      >  1:30153/1‑148 (MQ=255)                                         tttGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTcc                                                                                             <  2:30153/148‑1 (MQ=255)                                                   cgcTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTg                                                                                  <  2:40070/149‑1 (MQ=255)                                                     ctctGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGt                                                                                <  8:2794/149‑1 (MQ=255)                                                     ctctGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGt                                                                                >  7:32533/1‑149 (MQ=255)                                                          tgAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACt                                                                                                                                         >  8:78163/1‑87 (MQ=255)                                                          tgAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATa                                                                           <  8:32533/149‑1 (MQ=255)                                                            aaaGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACt                                                                         >  2:98687/1‑149 (MQ=255)                                                                    gAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCACGAATGAATTGGTCAATAATACCACACt                                                                 >  6:94116/1‑149 (MQ=255)                                                                        aCCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTa                                                             <  8:23615/149‑1 (MQ=255)                                                                          cTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCa                                                                              >  7:137904/1‑130 (MQ=255)                                                                          cTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCa                                                                              <  8:137904/130‑1 (MQ=255)                                                                                              gcTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGaaa                                       >  7:47250/1‑149 (MQ=255)                                                                                                    aGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGa                                 <  5:94116/149‑1 (MQ=255)                                                                                                      gTAAAGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACt                               >  1:149381/1‑149 (MQ=255)                                                                                                         aaGGATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCa                            >  2:30904/1‑149 (MQ=255)                                                                                                            gATCCATTGATGACGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATaa                         <  2:149381/149‑1 (MQ=255)                                                                                                                       gaCGAATAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACACAt              >  3:7891/1‑149 (MQ=255)                                                                                                                           aaTAAATGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATtata          >  6:159184/1‑149 (MQ=255)                                                                                                                                aTGGCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACTCATTATATGCtt     <  4:7891/149‑1 (MQ=255)                                                                                                                                   gCGAATCAATTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTc  <  1:98687/149‑1 (MQ=255)                                                                                                                                            |                                                                                                                                            ATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTC  >  NC_000913/754515‑754793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑