Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,722,202	T→G	D750E (GAT→GAG)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,722,202	0	T	G	100.0%	143.3 / NA	41	D750E (GAT→GAG)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/20);  total (21/20)

TGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTC  >  NC_000913/2722066‑2722334                                                                                                                                         |                                                                                                                                     tGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGc                                                                                                                          >  5:26508/1‑149 (MQ=255)   gCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTAc                                                                                                                             >  8:109454/1‑144 (MQ=255)   gCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTAc                                                                                                                             <  7:109454/144‑1 (MQ=255)           cccTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTttat                                                                                                                                >  5:78603/1‑133 (MQ=255)           cccTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTttat                                                                                                                                <  6:78603/133‑1 (MQ=255)                           cTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTAc                                                                                                >  2:67206/1‑149 (MQ=255)                                       gTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGAtt                                                                                     >  5:112166/1‑148 (MQ=255)                                        tAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGAtt                                                                                     >  5:170305/1‑147 (MQ=255)                                           aaTTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCGACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGc                                                                                 >  2:47395/1‑148 (MQ=255)                                             ttGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCaa                                                                              >  5:119251/1‑149 (MQ=255)                                             ttGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCaa                                                                              >  1:159317/1‑149 (MQ=255)                                               gAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTa                                                                                   <  6:127617/142‑1 (MQ=255)                                               gAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTa                                                                                   >  5:127617/1‑142 (MQ=255)                                                aaaaaCGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACa                                                                            <  6:125292/148‑1 (MQ=255)                                                  aaaCGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGa                                                                         >  2:40830/1‑149 (MQ=255)                                                  aaaCGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGa                                                                         <  6:170305/149‑1 (MQ=255)                                                           aCGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATc                                                                 <  6:112166/148‑1 (MQ=255)                                                                 ctTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTa                                                            <  6:119251/147‑1 (MQ=255)                                                                       ccGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCtt                                                                                                                                   <  2:50596/70‑1 (MQ=255)                                                                       ccGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCtt                                                                                                                                   >  1:50596/1‑70 (MQ=255)                                                                           ccGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAAt                                                >  5:9495/1‑149 (MQ=255)                                                                                   gCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGttt                                         <  1:47395/148‑1 (MQ=255)                                                                                    ccAGGAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTg                                        >  5:126917/1‑148 (MQ=255)                                                                                      agaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAg                                     <  2:159317/149‑1 (MQ=255)                                                                                           aTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTa                                >  1:139516/1‑149 (MQ=255)                                                                                               gCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTacgac                            <  1:40830/149‑1 (MQ=255)                                                                                                ccACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACTAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAg                                     <  2:13664/139‑1 (MQ=255)                                                                                                ccACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACTAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAg                                     >  1:13664/1‑139 (MQ=255)                                                                                                    aaCTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAtt                       <  1:67206/149‑1 (MQ=255)                                                                                                         cAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATc                   <  6:126917/148‑1 (MQ=255)                                                                                                          aGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCa                  >  5:107657/1‑148 (MQ=255)                                                                                                              aTTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACg              >  6:44190/1‑148 (MQ=255)                                                                                                                  aTTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGtt                                          <  3:70417/116‑1 (MQ=255)                                                                                                                  aTTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGtt                                          >  4:70417/1‑116 (MQ=255)                                                                                                                  aTTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAaga         <  6:26508/149‑1 (MQ=255)                                                                                                                     tCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAgaga       <  5:44190/148‑1 (MQ=255)                                                                                                                       gcgAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAt                        <  5:96542/129‑1 (MQ=255)                                                                                                                       gcgAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAt                        >  6:96542/1‑129 (MQ=255)                                                                                                                       gcgAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATc                   <  2:66795/134‑1 (MQ=255)                                                                                                                       gcgAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATc                   >  1:66795/1‑134 (MQ=255)                                                                                                                         gAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTc  <  6:9495/149‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTC  >  NC_000913/2722066‑2722334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑