Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,801,032	C→T	G323E (GGA→GAA)	waaR ←	lipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,801,032	0	C	T	100.0%	117.1 / NA	34	G323E (GGA→GAA)	waaR	lipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

TACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTG  >  NC_000913/3800894‑3801173                                                                                                                                           |                                                                                                                                              tACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGatat                                                                                                                                      >  4:49422/1‑148 (MQ=255)   cATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATaa                                                                                                                                    <  6:3062/148‑1 (MQ=255)             cTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCAc                                                                                                                         >  8:102396/1‑149 (MQ=255)                tGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTa                                                                                                                       >  7:45788/1‑148 (MQ=255)                            aTCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTaaaa                                                                                                                    >  6:48813/1‑139 (MQ=255)                            aTCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTaaaa                                                                                                                    <  5:48813/139‑1 (MQ=255)                               ggTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCt                                                                                                       >  2:115956/1‑149 (MQ=255)                                 tAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCttt                                                                                                     <  3:49422/149‑1 (MQ=255)                                    aaaCAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCtttttt                                                                                                  >  8:67881/1‑149 (MQ=255)                                    aaaCAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCtttttt                                                                                                  <  2:138498/149‑1 (MQ=255)                                    aaaCAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCtttttt                                                                                                  <  1:115956/149‑1 (MQ=255)                                                 tttGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCaataat                                                                                     >  8:106808/1‑149 (MQ=255)                                                                ctGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTg                                                                                                                            <  6:143549/95‑1 (MQ=255)                                                                ctGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTg                                                                                                                            >  5:143549/1‑95 (MQ=255)                                                                   aaTCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAatgatg                                                                                                                               >  1:60921/1‑89 (MQ=255)                                                                   aaTCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAatgatg                                                                                                                               <  2:60921/89‑1 (MQ=255)                                                                           taaACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTc                                                             <  1:192662/147‑1 (MQ=255)                                                                           taaACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTc                                                             >  2:192662/1‑147 (MQ=255)                                                                              aCCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCAt                                                           <  8:46953/146‑1 (MQ=255)                                                                              aCCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCAt                                                           >  7:46953/1‑146 (MQ=255)                                                                                  gTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATaa                                                                                                                                    <  2:171961/69‑1 (MQ=255)                                                                                         gTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAGGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGaa                                             >  3:56829/1‑149 (MQ=255)                                                                                            aTTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAAtt                                           <  8:45788/148‑1 (MQ=255)                                                                                               tACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTAAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTc                                        >  6:7853/1‑148 (MQ=255)                                                                                                 cGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCttttttt                                                                                                 >  6:172959/1‑89 (MQ=255)                                                                                                 cGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCttttttt                                                                                                 <  5:172959/89‑1 (MQ=255)                                                                                                  ggTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTa                                      >  6:9489/1‑147 (MQ=255)                                                                                                            tCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTta                           <  5:9489/148‑1 (MQ=255)                                                                                                              ggCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAg                        >  8:33611/1‑149 (MQ=255)                                                                                                                cAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTa                      <  7:106808/149‑1 (MQ=255)                                                                                                                          aGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGAt            <  7:33611/149‑1 (MQ=255)                                                                                                                                cTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATaa      <  6:41151/149‑1 (MQ=255)                                                                                                                                cTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATa       >  5:41151/1‑148 (MQ=255)                                                                                                                                    tataATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTg  >  3:60342/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                              TACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTG  >  NC_000913/3800894‑3801173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑