Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,260,401 G→A S673L (TCA→TTA)  tdcE ← pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,260,4010GA98.6% 219.8 / NA 73S673L (TCA→TTA) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (45/27);  minor base T (0/1);  total (45/28)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCC  >  NC_000913/3260258‑3260544
                                                                                                                                               |                                                                                                                                               
gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTaagaag                                                                                                                                            <  5:133813/149‑1 (MQ=255)
gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTaagaag                                                                                                                                            <  7:221074/149‑1 (MQ=255)
 cagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTTCCCAGCGCCGCAGGAACGATTAAGAAgg                                                                                                                                           >  8:81699/1‑149 (MQ=255)
 cagcaTTTCCCGATTCATTACGTTGACGTCGAGGTGTTGACCGCCTTCGACATCAGCTTCGTGGTGGAAATACCCATCCAGCAGGGCGACAAGCTTGGTTGTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGTAACCATTAAGAAgg                                                                                                                                           >  5:163296/1‑149 (MQ=255)
  agcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTGCGTGGTGGAAATACCCATCCAGCAGGACGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGgt                                                                                                                                          <  6:357154/149‑1 (MQ=255)
   gcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGgtg                                                                                                                                         <  4:304924/149‑1 (MQ=255)
          ccGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTAc                                                                                                                                      >  6:267913/1‑145 (MQ=255)
          ccGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACgaca                                                                                                                                  <  2:70287/149‑3 (MQ=255)
                  ttACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCt                                                                                                                          >  7:64958/1‑149 (MQ=255)
                     cgttgacgttgaGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTg                                                                                                                       <  6:58751/149‑1 (MQ=255)
                     cgttgacgttgaGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTg                                                                                                                       <  8:72526/149‑1 (MQ=255)
                       ttgacgttgaGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGc                                                                                                                     <  7:388239/149‑1 (MQ=255)
                             ttgaGGTGTTGACCGCCTTCGACATCCGATTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGt                                                                                                               <  5:381459/149‑1 (MQ=255)
                                    gtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACgg                                                                                                         >  4:270968/1‑148 (MQ=255)
                                    gtTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGAGTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACgg                                                                                                         >  5:187758/1‑148 (MQ=255)
                                    gtTGACCGCCTTCGACATCCGCATCGTGGTGGAAATAACCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCTCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACgt                                                                                                         >  5:229345/1‑147 (MQ=255)
                                     ttGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACgg                                                                                                         >  5:190806/1‑147 (MQ=255)
                                          cGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTc                                                                                                  <  1:115516/149‑1 (MQ=255)
                                          cGCATTCGTAATCAGCATGGTGGTGGTATAACACATGCAGCAGGGCGAGTAGGTTGGGTTTACGTACTGGATCTTCTTTGGCCTGCTCCGCAGGAACGATTAATAATGTGTACTAGATATCATCTTTGGCGTATTTTAAAGGCAGTTTc                                                                                                  <  8:154713/149‑1 (MQ=255)
                                            ccTTCGTCATCTGCTTCGTTGTGGAAATACACATCCTGCAGGCCGTCAAGGTTGGTTTTACGTAATGGATCTTCTTTGCCCAGCGCCGCAGGATCGATTTAGAAGGTGTACGAGTTCACATCTTTGGCTTAGGTGAACGGCAGTTTCGc                                                                                                <  4:103567/149‑1 (MQ=255)
                                            ccTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGc                                                                                                >  3:103567/1‑149 (MQ=255)
                                            ccTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGc                                                                                                >  7:233344/1‑149 (MQ=255)
                                                 gACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTAc                                                                                                                                      <  6:199251/106‑1 (MQ=255)
                                                 gACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTAc                                                                                                                                      >  5:199251/1‑106 (MQ=255)
                                                 gACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGTCGTTGGTGAACGGCAGTTACGCCACCg                                                                                           >  1:179163/1‑149 (MQ=255)
                                                       cGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTc                                                                                      >  3:49813/1‑148 (MQ=255)
                                                        gCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCaa                                                                                    >  5:202606/1‑149 (MQ=255)
                                                            cGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGGGAACGGCAGTTTCGCCACCGACGTCAATGa                                                                                 <  3:16770/148‑1 (MQ=255)
                                                              tggtggAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGCTCTTCTTTTCCCAGCTCCGCAGGAACGATTAAGAAGGGGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc                                                                              >  7:281588/1‑149 (MQ=255)
                                                                gtggAAATACCCATCCAGCAGGCAGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCa                                                                            >  6:375353/1‑149 (MQ=255)
                                                                 tggAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCAc                                                                           <  6:202606/149‑1 (MQ=255)
                                                                   gAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACgg                                                                         <  5:267913/149‑1 (MQ=255)
                                                                    aaaTACCCATCCAGCAGGCCGACAAGGTTGGTTTAACGTACTGGATCTTCTTTGCCAAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGAGCCCATCATTGGGGTAGGGGAACGGCAGTTTCGCCACCGACGTCAAGCAGGCCACGGc                                                                        >  5:310253/1‑149 (MQ=255)
                                                                    aaaTACCCATCCAGCAGGCCGACAAGGTTGGTGTTACGTACTGGATCTTCTTTGCCCAGCGCCACAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGc                                                                        <  2:50339/149‑1 (MQ=255)
                                                                          ccATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCttt                                                                  >  4:213138/1‑149 (MQ=255)
                                                                          ccATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCttt                                                                  >  6:80223/1‑149 (MQ=255)
                                                                            aTCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGt                                                                                       <  1:221389/126‑1 (MQ=255)
                                                                            aTCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGt                                                                                       >  2:221389/1‑126 (MQ=255)
                                                                             tCCAGCCGGCCGACAAGGTTGGTGTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCg                                                               <  2:399183/149‑1 (MQ=255)
                                                                             tCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGCCGTCAATGAGGCCACGGCACCTTTGCg                                                               >  5:71296/1‑149 (MQ=255)
                                                                               cagcagGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCgg                                                              <  6:229345/148‑1 (MQ=255)
                                                                                agcagGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGt                                                             >  6:173752/1‑148 (MQ=255)
                                                                                agcagGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTc                                                            >  6:201865/1‑149 (MQ=255)
                                                                                 gcagGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACTATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATg                                                                                  >  4:34693/1‑126 (MQ=255)
                                                                                 gcagGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACTATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATg                                                                                  <  3:34693/126‑1 (MQ=255)
                                                                                       cGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCa                                                     >  1:193093/1‑149 (MQ=255)
                                                                                         aCAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGa                                                        <  4:355174/144‑1 (MQ=255)
                                                                                         aCAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGa                                                        >  3:355174/1‑144 (MQ=255)
                                                                                            tggttgcttTTACGTACTGGATGTTCTTTGCCCAGCGCAGCAGGAACTCTTAAGAATGTGTACGAGATCCCATCTTTGGCGTAGGTGATCGGCAGTTTCGCCACCGACGTCAATGAGGCCTCGGGACCTTTGCGGTCACGACCATGCAt                                                <  4:28539/148‑1 (MQ=255)
                                                                                                 ggttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGt                                           >  6:265772/1‑149 (MQ=255)
                                                                                                   ttttACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCCCGGCACCTTTTCGGTCACGACCAAGCATCGGGt                                           >  6:215735/1‑147 (MQ=255)
                                                                                                    tttACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAg                                        >  5:91883/1‑149 (MQ=255)
                                                                                                               gATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGgcgc                              >  5:348027/1‑148 (MQ=255)
                                                                                                               gATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGgcgc                              >  5:262068/1‑148 (MQ=255)
                                                                                                               gATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGgcgc                              <  5:80223/148‑1 (MQ=255)
                                                                                                                   ttcttTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACg                         >  6:206565/1‑149 (MQ=255)
                                                                                                                     cttTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGgt                       >  8:295467/1‑149 (MQ=255)
                                                                                                                     cttTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGgt                       >  1:227299/1‑149 (MQ=255)
                                                                                                                     cttTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGgt                       >  5:387453/1‑149 (MQ=255)
                                                                                                                     cttTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGAAGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACg                         <  5:375353/147‑1 (MQ=255)
                                                                                                                          cccAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTcc                  >  5:225677/1‑149 (MQ=255)
                                                                                                                          cccAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCCACACCGACGTCAAAGAGGACCCGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCCCCCGGCGCGAACGGTGTTcc                  >  1:150198/1‑149 (MQ=255)
                                                                                                                            cAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGCGGCCACGGCAACTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGGGTTcctg                >  8:113804/1‑147 (MQ=255)
                                                                                                                            cAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCgg                >  7:329758/1‑149 (MQ=255)
                                                                                                                             aGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGgc               >  4:9533/1‑149 (MQ=255)
                                                                                                                               cgccgcAGGAACGATTAAGACGGTGTACGAGCTCCCAGCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTCCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGgcgc             <  3:187752/149‑1 (MQ=255)
                                                                                                                               cgccgcAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGgcgc             <  2:227299/149‑1 (MQ=255)
                                                                                                                                 ccgcAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGcga           >  2:295834/1‑149 (MQ=255)
                                                                                                                                 ccgcAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGcga           <  2:193093/149‑1 (MQ=255)
                                                                                                                                  cgcAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGcgac          >  5:333570/1‑149 (MQ=255)
                                                                                                                                    cAGGAACGATTAAGCCGGGGTACGAGATCCCATCTTTGGCGTAGGGGCACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGTTTAGCGCCCGGCGCGAACGGTGTTCCGGCGcgacga        <  7:113804/149‑1 (MQ=255)
                                                                                                                                      ggAACGATTAAGAAGGTGTAGGAGATCCCATGTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGAcc      >  4:243131/1‑149 (MQ=255)
                                                                                                                                        aaCGATTAAGCAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGt    >  5:357707/1‑149 (MQ=255)
                                                                                                                                          cGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTcc  >  7:335541/1‑149 (MQ=255)
                                                                                                                                          cGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCAGGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTcc  <  6:190806/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCC  >  NC_000913/3260258‑3260544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: