Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	169,686	Δ1 bp	coding (2203/2244 nt)	fhuA →	ferrichrome outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	169,686	0	G	.	100.0%	195.5 / NA	43	coding (2203/2244 nt)	fhuA	ferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/20);  total (23/20)

ATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATA  >  NC_000913/169558‑169826                                                                                                                                 |                                                                                                                                             aTGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTgcaa                                                                                                                         <  7:23953/149‑1 (MQ=255)  tGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTgcaac                                                                                                                        <  2:69917/149‑1 (MQ=255)  tGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTgcaac                                                                                                                        >  2:57933/1‑149 (MQ=255)                  gATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTc                                                                                                        >  8:114591/1‑149 (MQ=255)                      tGGCGCGAGTTGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAAt                                                                                                    >  4:150139/1‑149 (MQ=255)                                cGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTg                                                                                          <  4:100287/149‑1 (MQ=255)                                 ggCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACTCTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTg                                                                                          <  7:88744/148‑1 (MQ=255)                                  gCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTggg                                                                                        <  1:57933/149‑1 (MQ=255)                                               cAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCg                                                                           <  7:114591/149‑1 (MQ=255)                                                 aCGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTcc                                                                            <  3:119783/146‑1 (MQ=255)                                                 aCGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTcc                                                                            >  4:119783/1‑146 (MQ=255)                                                           gCATGTTATCAACCTGTTCGAGCGTGAATACGTCGCCAGCTGCTTTCACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGGCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTgg                                                                                         <  7:32371/123‑1 (MQ=255)                                                           gCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGACAGGTCGTTGCAACCGCACCCTTCCGTTTCTAATTTCTCTTTAgg                                                                                         >  8:32371/1‑123 (MQ=255)                                                            cATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTcac                                                               >  8:142461/1‑148 (MQ=255)                                                              tGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAg                                                            >  5:117756/1‑149 (MQ=255)                                                                  aacaacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTgggg                                                                                       >  2:160723/1‑118 (MQ=255)                                                                  aacaacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTgggg                                                                                       <  1:160723/118‑1 (MQ=255)                                                                   acaacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTAAGGCTGCTACTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCAGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGc                                                       >  7:61652/1‑149 (MQ=255)                                                                       ccTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGt                                                    >  8:117857/1‑148 (MQ=255)                                                                         tGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTcc                                                                            >  8:83423/1‑122 (MQ=255)                                                                         tGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTcc                                                                            <  7:83423/122‑1 (MQ=255)                                                                                     aaTACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGc                                               >  2:162925/1‑139 (MQ=255)                                                                                     aaTACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGc                                               <  1:162925/139‑1 (MQ=255)                                                                                     aaTACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATacac                                     <  3:150139/149‑1 (MQ=255)                                                                                        aCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCtt                                                                                                               >  8:95243/1‑72 (MQ=255)                                                                                        aCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCtt                                                                                                               <  7:95243/72‑1 (MQ=255)                                                                                         cGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAAt                                 >  1:172531/1‑149 (MQ=255)                                                                                         cGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGAGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAAt                                 <  7:117857/149‑1 (MQ=255)                                                                                          gTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTc                                                                                                        >  6:174153/1‑77 (MQ=255)                                                                                          gTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTc                                                                                                        <  5:174153/77‑1 (MQ=255)                                                                                          gTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAAt                                 >  6:90758/1‑148 (MQ=255)                                                                                                       ttAACACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCAc                   >  8:121123/1‑149 (MQ=255)                                                                                                         aaCACTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACtt                 >  7:121419/1‑149 (MQ=255)                                                                                                          acacTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCa                                              >  1:110834/1‑119 (MQ=255)                                                                                                          acacTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCa                                              <  2:110834/119‑1 (MQ=255)                                                                                                          acacTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACtt                 <  5:90758/148‑1 (MQ=255)                                                                                                             cTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATacac                                     >  2:73151/1‑125 (MQ=255)                                                                                                             cTTATGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATacac                                     <  1:73151/125‑1 (MQ=255)                                                                                                                 tGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACt          <  7:142461/148‑1 (MQ=255)                                                                                                                 tGGCTGCTTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTg         >  7:63533/1‑149 (MQ=255)                                                                                                                   gctgctTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGc        <  6:117756/148‑1 (MQ=255)                                                                                                                    ctgctTCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAg                                             >  2:53048/1‑110 (MQ=255)                                                                                                                        ttCTGGGGC‑CAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAata  >  8:30709/1‑149 (MQ=255)                                                                                                                                 |                                                                                                                                             ATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATA  >  NC_000913/169558‑169826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑