Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 141,212 T→A 5.3% N5I (AAT→ATT)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913141,2120TA 5.3% 243.0 / 3.6 75N5I (AAT→ATT) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (35/36);  new base A (2/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

CGACGCCGAGCATCACAAGGCCAGCGATAGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGT  >  NC_000913/141066‑141357
                                                                                                                                                  |                                                                                                                                                 
cGACGCCGAGCATCACACGGCCAGCTATTGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCTCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGTGTAATCGTCGCGAGCCTGTATTg                                                                                                                                                 <  8:330344/149‑1 (MQ=255)
    gCCGAGCATCACAAGGCCAGCGATAGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTaa                                                                                                                                             <  7:245898/149‑1 (MQ=255)
     ccGAGCATCACAAGGCCAGCGATAGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTCTTAAt                                                                                                                                            >  7:100112/1‑149 (MQ=255)
          cATCACAAGGCCAGCGATAGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCa                                                                                                                                       <  7:34790/149‑1 (MQ=255)
            tCACAAGGCCAGCGATAGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTg                                                                                                                                          >  4:191094/1‑144 (MQ=255)
            tCACAAGGCCAGCGATAGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTg                                                                                                                                          <  3:191094/144‑1 (MQ=255)
                aaGGCCAGCGATAGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAaga                                                                                                                                 <  2:334197/149‑1 (MQ=255)
                  ggCCAGCGATAGGGTTGTACAAGGATCCGCCAATGGCGACCAGCCAGCCTCCGCCAATGAGTAGATACATCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAgaga                                                                                                                               <  8:100112/149‑1 (MQ=255)
                          aTAGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCAtt                                                                                                                        >  6:134139/1‑148 (MQ=255)
                              ggTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCaa                                                                                                                    <  6:353867/148‑1 (MQ=255)
                                  gtaCCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCa                                                                                                                <  7:268670/148‑1 (MQ=255)
                                    aCCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCAtt                                                                                                              >  8:314254/1‑148 (MQ=255)
                                     ccAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTaa                                                                                                            >  7:288477/1‑149 (MQ=255)
                                     ccAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTaa                                                                                                            >  8:356740/1‑149 (MQ=255)
                                            ccgccAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTta                                                                                                     <  5:134139/149‑1 (MQ=255)
                                             cgccAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTtag                                                                                                    <  7:314254/149‑1 (MQ=255)
                                               ccAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCaa                                                                                                                    <  6:29929/131‑1 (MQ=255)
                                               ccAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCaa                                                                                                                    >  5:29929/1‑131 (MQ=255)
                                               ccAATCGCGACCAGCCACCCTCCGCAATTGAGTAGAAACACCCCGCAAAGCGCTGCAAACCGAGCAGTTAGCCTGCCCTGTATTCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCTTTAATATTTtagta                                                                                                  <  2:363814/149‑1 (MQ=255)
                                                 aaTCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTtagtag                                                                                                 >  2:59638/1‑148 (MQ=255)
                                                          cagccagcCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAattaatta                                                                                       >  7:352579/1‑149 (MQ=255)
                                                          cagccagcCTCCGCAAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAattaatta                                                                                       <  8:303294/149‑1 (MQ=255)
                                                            gccagccTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAattaatta                                                                                       >  4:128069/1‑147 (MQ=255)
                                                              cagccTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAgg                                                                                   <  3:128069/149‑1 (MQ=255)
                                                               agccTCCGCCAATGAGTAGATAAATACCGCAAAGCGATGCAAAAAGGGCTGATAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACAATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGt                                                                                  >  1:182394/1‑149 (MQ=255)
                                                                gccTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGtt                                                                                 <  7:348019/149‑1 (MQ=255)
                                                                  cTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGttt                                                                                >  1:94817/1‑148 (MQ=255)
                                                                   tCCGCCAATGAGTAGATACCGCCCGCAACGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGCGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGtttt                                                                               >  4:47260/1‑148 (MQ=255)
                                                                          aTGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTAATGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACAtgtg                                                                       >  7:125916/1‑149 (MQ=255)
                                                                            gAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACAtgtgt                                                                      >  1:405556/1‑148 (MQ=255)
                                                                             aGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGa                                                                    <  3:312985/149‑1 (MQ=255)
                                                                              gTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGAt                                                                   >  2:131516/1‑149 (MQ=255)
                                                                                  aTACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGAGCCGAGTAATCGTCGCGAGCCAGTATTGGTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGAAATTAATTATATGTTTTAACATGTGTGATCGt                                                                >  8:440642/1‑148 (MQ=255)
                                                                                    acggCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGtcat                                                             >  5:382757/4‑149 (MQ=255)
                                                                                       gCCCGCAAAGAGCTGCAAAAAGGGCAATTAGCGTGACAAGAAATCGTCGCGAGCCAGTATTGTTAATAGCCATAAAGAGAAACCATTTCAATACAATAATAATTTAGTAGCAATTAATTATATGATTTAACATGAGTGATCTtcatca                                                           >  4:300470/1‑148 (MQ=255)
                                                                                        cccGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATcac                                                          >  1:382528/1‑148 (MQ=255)
                                                                                        cccGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATcac                                                          >  6:324254/1‑148 (MQ=255)
                                                                                                gcgcTGCAAAAACGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGc                                                 <  2:435291/149‑1 (MQ=255)
                                                                                                   cTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCttt                                              <  8:352579/149‑1 (MQ=255)
                                                                                                    tGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTtta                                             >  6:219164/1‑149 (MQ=255)
                                                                                                      cAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGa                                                   >  2:212035/1‑141 (MQ=255)
                                                                                                      cAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGa                                                   <  1:212035/141‑1 (MQ=255)
                                                                                                      cAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTttatt                                           <  2:405556/149‑1 (MQ=255)
                                                                                                        aaaaGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTta                                                                                                     <  8:119685/89‑1 (MQ=255)
                                                                                                        aaaaGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTta                                                                                                     >  7:119685/1‑89 (MQ=255)
                                                                                                        aaaaCGGCTGTTAGCGTTACGAGTAATCGTCTCTAGCCTGTATTGTTAATTGCCATAAAGATACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCCTCACAATTCGAGCTTTATTaa                                         <  5:351565/149‑1 (MQ=255)
                                                                                                         aaaGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAAt                                                       <  5:18600/134‑1 (MQ=255)
                                                                                                         aaaGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAAt                                                       >  6:18600/1‑134 (MQ=255)
                                                                                                          aaGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACa                                       <  2:382528/149‑1 (MQ=255)
                                                                                                          aaGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTAATGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACa                                       <  8:125916/149‑1 (MQ=255)
                                                                                                               cTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAGTTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTc                                  >  4:82310/1‑149 (MQ=255)
                                                                                                               cTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTc                                  >  3:336958/1‑149 (MQ=255)
                                                                                                                tGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTcc                                 >  1:212344/1‑149 (MQ=255)
                                                                                                                  ttAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCcgc                               >  1:172808/1‑149 (MQ=255)
                                                                                                                  ttAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCcgc                               <  5:210386/149‑1 (MQ=255)
                                                                                                                  ttAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGGCATCACAATTCGAGCTTTATTAACAGATTCcgc                               <  1:59638/149‑1 (MQ=255)
                                                                                                                     gCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAAc                                        <  6:29619/137‑1 (MQ=255)
                                                                                                                     gCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAAc                                        >  5:29619/1‑137 (MQ=255)
                                                                                                                      cGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCgaat                           <  5:324254/149‑1 (MQ=255)
                                                                                                                       gTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCgaatg                          <  6:15600/149‑1 (MQ=255)
                                                                                                                          aCGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAaga                                                                                                                                 <  5:118586/43‑1 (MQ=255)
                                                                                                                          aCGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAaga                                                                                                                                 >  6:118586/1‑43 (MQ=255)
                                                                                                                           cGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATa                      <  8:288477/149‑1 (MQ=255)
                                                                                                                           cGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATa                      <  6:382757/149‑1 (MQ=255)
                                                                                                                              gTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAAATCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGtt                   >  7:320119/1‑149 (MQ=255)
                                                                                                                               tAATCGTCGCGAGCCTGTATTGTT‑ATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGttt                  >  4:231305/1‑148 (MQ=255)
                                                                                                                               tAATCGTCGCGAGCCTGTAATGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAg                     <  3:385529/146‑1 (MQ=255)
                                                                                                                               tAATCGTCGCGAGCCTGTAATGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAg                     >  4:385529/1‑146 (MQ=255)
                                                                                                                                 aTCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAAATATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGtttt                 >  1:386741/1‑148 (MQ=255)
                                                                                                                                    gtcgCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTg             <  1:131516/149‑1 (MQ=255)
                                                                                                                                       gcgAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGta          >  2:410362/1‑149 (MQ=255)
                                                                                                                                       gcgAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGta          >  4:94766/1‑149 (MQ=255)
                                                                                                                                             cTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGc    <  2:94817/149‑1 (MQ=255)
                                                                                                                                                tATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCgt  <  4:336958/148‑1 (MQ=255)
                                                                                                                                                tATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCgt  <  2:172808/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGACGCCGAGCATCACAAGGCCAGCGATAGGGTAGTACCAGGAGCCGCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGT  >  NC_000913/141066‑141357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑