Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,270,668 G→A 5.1% intergenic (+208/+81) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,270,6680GA 5.1% 319.7 / 3.4 100intergenic (+208/+81)rdlC/chaAsRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (51/43);  new base A (3/2);  total (55/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

Only 100 of 102 total aligned reads displayed.

GCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAG  >  NC_000913/1270520‑1270816
                                                                                                                                                    |                                                                                                                                                    
gCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTg                                                                                                                                                      >  5:327520/1‑149 (MQ=255)
     cTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAg                                                                                                                                                 >  3:10277/1‑149 (MQ=255)
               aTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATg                                                                                                                                         >  8:188709/1‑147 (MQ=255)
                 actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATgaag                                                                                                                                      >  2:382940/1‑148 (MQ=255)
                 actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATgaag                                                                                                                                      >  2:248724/1‑148 (MQ=255)
                   tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGaaa                                                                                                                                   >  2:60987/1‑149 (MQ=255)
                   tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGaaa                                                                                                                                   >  3:35131/1‑149 (MQ=255)
                   tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGaaa                                                                                                                                   >  2:86684/1‑149 (MQ=255)
                     cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGaaa                                                                                                                                   <  1:36060/147‑1 (MQ=255)
                     cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATa                                                                                                                                 <  6:327520/149‑1 (MQ=255)
                           aaTTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAAt                                                                                                                             >  3:370067/1‑147 (MQ=255)
                            aTTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAg                                                                                                                          <  7:162588/149‑1 (MQ=255)
                             ttCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGATCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAg                                                                                                                          >  6:168170/1‑148 (MQ=255)
                               cATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCa                                                                                                                        <  1:86684/148‑1 (MQ=255)
                                aTCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAg                                                                                                                       <  7:233700/148‑1 (MQ=255)
                                 tCTTCACTTCATCCCTGAAATGTTTGCAATGAAGATTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTATCCAGGTAGTATGAAGAAATACAATa                                                                                                                            <  7:113158/142‑1 (MQ=255)
                                 tCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTATCCAGGTAGTATGAAGAAATACAATa                                                                                                                            >  8:113158/1‑142 (MQ=255)
                                 tCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTCCAGTGGTTTCGTGCACGGTGTGGTACAGGCTCGCAATTCTGATTAAAGAAGAGTCCAGGTAGTATTAAGAAATACAATAAGCAgg                                                                                                                      >  5:337316/1‑148 (MQ=25)
                                   ttcacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAAt                                                                                                                   >  2:88723/1‑149 (MQ=255)
                                   ttcacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTATGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAAt                                                                                                                   >  3:143880/1‑149 (MQ=255)
                                   ttcacttcaTCCCTGAAATGTTTGCAAAGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGCTGTGGTACAAGCTCGCAATTCTGATTAACGACCTATCCAGGTAGTATGAAGAAATACAATAAGAAGGAAt                                                                                                                   >  3:24544/1‑149 (MQ=37)
                                        ttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTtata                                                                                                              >  3:152427/1‑149 (MQ=255)
                                        ttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTtata                                                                                                              >  1:399386/1‑149 (MQ=255)
                                          caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTatat                                                                                                             >  7:431062/1‑148 (MQ=255)
                                          caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTatat                                                                                                             <  4:370067/148‑1 (MQ=255)
                                          caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTatat                                                                                                             <  1:382940/148‑1 (MQ=255)
                                           cggtatGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTAtatta                                                                                                           <  7:273909/144‑1 (MQ=255)
                                              ccTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTAtc                                                                                                         <  5:211481/148‑1 (MQ=255)
                                              ccTGAAATGTTTGCAATGAAGAGTACATTACGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTACAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTAtct                                                                                                        >  4:82647/1‑149 (MQ=255)
                                               cTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGACTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTAtctc                                                                                                       >  6:337517/1‑149 (MQ=255)
                                                tGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTcc                                                                                                      >  2:135164/1‑149 (MQ=255)
                                                  ataTGTTTGCAATGTAGAGTACATTCCGGCTTTTCAAGAGCTGTTGCAGTGGTTTCGTGCTGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATTCTATAATCAGGAAttt                                                                                                                 <  2:135790/134‑1 (MQ=38)
                                                  aaaTGTTTGCAATGAAGAGTACATTCCGGCTATTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCAGTCCAGCTAGTATGAAGAAATACAATAAGAAGGAAttt                                                                                                                 >  1:135790/1‑136 (MQ=255)
                                                       tttGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGAt                                                                                                >  6:78054/1‑148 (MQ=255)
                                                         tGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTAc                                                                                             >  5:207047/1‑149 (MQ=255)
                                                          gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTAcc                                                                                            >  6:118043/1‑149 (MQ=255)
                                                          gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTAcc                                                                                            >  1:36482/1‑149 (MQ=255)
                                                          gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTAcc                                                                                            >  7:66290/1‑149 (MQ=255)
                                                           cAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCg                                                                                           >  2:415634/1‑149 (MQ=255)
                                                           cAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGGGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTAcc                                                                                            <  2:357211/148‑1 (MQ=255)
                                                             aTGAAGAGTAAATTCCGTATTTTCAACAGCTGTTGAACTGGTTTCGTGCAGGGTGTGGTACAGGCTCGAAATTCTGATTAACGACCTGTCCAGGTAGTTTGAGGAAATACAATAAGCAGGAAATTATATTATCTCCCACGATTACCGt                                                                                          >  8:162885/1‑148 (MQ=255)
                                                                  gagTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCg                                                                                     >  5:364037/1‑148 (MQ=255)
                                                                        aTTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCggag                                                                                  >  6:188712/1‑145 (MQ=255)
                                                                        aTTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCggag                                                                                  >  2:119838/1‑145 (MQ=255)
                                                                        aTTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGgaga                                                                              <  2:399386/149‑1 (MQ=255)
                                                                            cGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATTAAGAAATACAATAAGCATGAATTTATATTATCTCCACCGATTACAGTCATCGGAGGAGatat                                                                           >  3:210252/1‑148 (MQ=255)
                                                                               cTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                               <  5:58285/141‑1 (MQ=255)
                                                                               cTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                               >  6:58285/1‑141 (MQ=255)
                                                                                ttttCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACt                                                                       >  7:175450/1‑148 (MQ=255)
                                                                                  ttCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACt                                                                       <  8:175450/146‑1 (MQ=255)
                                                                                   tCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGt                                                                     >  2:214320/1‑147 (MQ=255)
                                                                                    cAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATGTTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCa                                                                   <  1:60987/148‑1 (MQ=255)
                                                                                    cAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCa                                                                   <  5:188712/148‑1 (MQ=255)
                                                                                         gCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCaaa                                                             <  6:364037/149‑1 (MQ=255)
                                                                                             ttGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAata                                                           <  1:196146/147‑1 (MQ=255)
                                                                                             ttGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATc                                                         <  6:13572/149‑1 (MQ=255)
                                                                                             ctGCAGTGCTTTCGTGGAGGGTGGTGTACAGGCTCGCAATTCTGAATAACGACCTGTCCTGGTACTATGTAGAAATACAATAAGCTCGAAATTATATTATCTCCCCCGATTACCGTCATCTGAGGAGATATAACTGTCAGGCAAATTTc                                                         <  6:337316/148‑1 (MQ=255)
                                                                                               gCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGACTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCg                                                        <  5:337517/148‑1 (MQ=255)
                                                                                                cAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtc                                                      <  8:66290/149‑1 (MQ=255)
                                                                                                cAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtc                                                      <  5:102160/149‑1 (MQ=255)
                                                                                                   tGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtcatc                                                   <  4:152427/149‑1 (MQ=255)
                                                                                                   tGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtcat                                                    >  3:290759/1‑148 (MQ=255)
                                                                                                   aggTATCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTCTCCAGGTAGAATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtcat                                                    >  6:361285/2‑148 (MQ=255)
                                                                                                          gTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAgg                                            <  1:248724/149‑1 (MQ=255)
                                                                                                           tGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggc                                           <  5:168170/149‑1 (MQ=255)
                                                                                                           tGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggc                                           <  2:36482/149‑1 (MQ=255)
                                                                                                              aGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggcgg                                         >  8:160186/1‑148 (MQ=255)
                                                                                                               gggTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggcggc                                        >  4:230406/1‑148 (MQ=255)
                                                                                                                ggTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCa                                       >  6:217404/1‑148 (MQ=255)
                                                                                                                 gtgtGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCaaa                                     <  5:217404/149‑1 (MQ=255)
                                                                                                                      gTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGt                                 <  6:207047/148‑1 (MQ=255)
                                                                                                                      gTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTg                                <  3:230406/149‑1 (MQ=255)
                                                                                                                      gTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTg                                >  8:321453/1‑149 (MQ=255)
                                                                                                                       tACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCaa                                                 >  7:373498/1‑131 (MQ=255)
                                                                                                                       tACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCaa                                                 <  8:373498/131‑1 (MQ=255)
                                                                                                                       tACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGc                               <  1:119838/149‑1 (MQ=255)
                                                                                                                          aGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGc                               >  5:172075/1‑146 (MQ=255)
                                                                                                                          aGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGc                               >  2:244715/1‑146 (MQ=255)
                                                                                                                            gCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAt                          <  3:82647/149‑1 (MQ=255)
                                                                                                                             cTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtg                         >  6:146169/1‑149 (MQ=255)
                                                                                                                              tCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgc                        >  2:172913/1‑149 (MQ=255)
                                                                                                                                gCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTc                                                                    <  4:373490/103‑1 (MQ=255)
                                                                                                                                gCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTc                                                                    >  3:373490/1‑103 (MQ=255)
                                                                                                                                   aTTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgcgctgc                   <  5:78054/149‑1 (MQ=255)
                                                                                                                                    ttCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTg                                <  7:66998/135‑1 (MQ=255)
                                                                                                                                    ttCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTg                                >  8:66998/1‑135 (MQ=255)
                                                                                                                                    ttCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgcgctgcg                  >  8:230442/1‑149 (MQ=255)
                                                                                                                                    ttCTGATTAACGACCTATCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATc                                                         >  2:221699/1‑110 (MQ=255)
                                                                                                                                    ttCTGATTAACGACCTATCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATc                                                         <  1:221699/110‑1 (MQ=255)
                                                                                                                                       tGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTTCCAGATGCGCTGCGCCa               >  2:360005/1‑149 (MQ=255)
                                                                                                                                       tGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCa               >  6:297207/1‑149 (MQ=255)
                                                                                                                                        gATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCAt              >  5:196099/1‑149 (MQ=255)
                                                                                                                                         aTTAACGTCGTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCGCGATTACCGTCATCGGAGGAGTTATAACTGTCAGGCAAATTTCGTCATCAAATAGGCGGCAAACTGTGCCAGATGCGCTGCGCCAtt             <  8:431062/149‑1 (MQ=255)
                                                                                                                                         aTTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCa               <  1:135164/147‑1 (MQ=255)
                                                                                                                                         aTTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCAtt             <  1:172913/149‑1 (MQ=255)
                                                                                                                                          ttAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg            <  7:188709/149‑1 (MQ=255)
                                                                                                                                                aCCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCGTCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGa           >  6:103896/1‑144 (MQ=255)
                                                                                                                                                aCCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCGTCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGa           <  5:103896/144‑1 (MQ=255)
                                                                                                                                                 ccTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGt     <  1:214320/149‑1 (MQ=255)
                                                                                                                                                    gTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAg  >  6:128044/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAG  >  NC_000913/1270520‑1270816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: