Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,753,767 C→A 6.2% E22* (GAA→TAA)  ydhV ← putative oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,753,7670CA 6.2% 209.0 / 2.9 64E22* (GAA→TAA) ydhVputative oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base C (33/27);  new base A (2/2);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

GCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCA  >  NC_000913/1753622‑1753913
                                                                                                                                                 |                                                                                                                                                  
gCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGa                                                                                                                                                  <  8:381327/148‑1 (MQ=255)
   ccAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAggg                                                                                                                                               >  2:20475/1‑148 (MQ=255)
          cAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCTCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATAAATAATTTTGTAGAAGAAGCCCATGCCACCGAAAAAACTTTTAAAATTACTGGAATCTTCGAGGGTAAAAtt                                                                                                                                        >  6:41406/1‑148 (MQ=255)
           agatAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTc                                                                                                                                      <  5:387197/146‑1 (MQ=255)
            gTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTcc                                                                                                                                     <  3:142/149‑1 (MQ=255)
               aaTGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGt                                                                                                                                  >  4:366084/1‑149 (MQ=255)
                aTGGGCCGGTAGCAAAGACTAATTTCTTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGt                                                                                                                                  <  6:197404/148‑1 (MQ=255)
                 tGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCg                                                                                                                                >  7:102931/1‑149 (MQ=255)
                    gCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTga                                                                                                                             <  3:322607/149‑1 (MQ=255)
                    gCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTga                                                                                                                             <  2:157344/149‑1 (MQ=255)
                             aaaGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGActc                                                                                                                    >  5:14232/1‑149 (MQ=255)
                             aaaGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGActc                                                                                                                    >  5:19845/1‑149 (MQ=255)
                             aaaGACTAATTAATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTTGCCGAAGCCCATGCCACCGACAAATCTTTTAAACTTACTGTAATCTTCGAGGGTAATATTTCCTGTCGTGAGAGTGActc                                                                                                                    <  2:295454/149‑1 (MQ=255)
                                 aCTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTaa                                                                                                                <  2:153618/149‑1 (MQ=255)
                                 aCTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTaa                                                                                                                >  7:9045/1‑149 (MQ=255)
                                   tAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGActc                                                                                                                    <  6:310961/143‑1 (MQ=255)
                                   tAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGActc                                                                                                                    >  5:310961/1‑143 (MQ=255)
                                     aTTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAatat                                                                                                             <  1:20475/148‑1 (MQ=255)
                                     aTTTATTCGCTTCATCGAAAGGATTCGTGCCTTGCGGTACTTCGTCATACATAATTTTGTAGCCGCAGCCCATGCCACCGACAAAACTTTTAACCATACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAatata                                                                                                            <  5:106009/149‑1 (MQ=255)
                                         aTTCGCTTCATCGAAAGGTTTCGTGCCTGGCGTTACTTCGTCATACCTAATTTTGTAGCCGCAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAAGCTTCGAGGGTAATATTTCCTGTCGTGAGATGGACTCTTAATATATTa                                                                                                         <  3:320591/148‑1 (MQ=255)
                                            cGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCt                                                                                                      <  8:102931/148‑1 (MQ=255)
                                                 cATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGCCAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAAAATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTTTcc                                                                                                  <  4:310159/147‑1 (MQ=255)
                                                   tCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCt                                                                                                      <  3:194451/141‑1 (MQ=255)
                                                   tCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCt                                                                                                      >  4:194451/1‑141 (MQ=255)
                                                   tCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTAGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAat                                                                                                               >  1:204303/1‑132 (MQ=255)
                                                   tCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTAGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAat                                                                                                               <  2:204303/132‑1 (MQ=255)
                                                   tCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTAGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAat                                                                                                               <  2:198151/132‑1 (MQ=255)
                                                   tCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTAGAGGGTAATATTTCCTGTCGTGAGATTGACTATTAat                                                                                                               >  1:198151/1‑132 (MQ=255)
                                                     gAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGt                                                                                            >  8:262199/1‑149 (MQ=255)
                                                        aGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAg                                                                                         >  2:164578/1‑149 (MQ=255)
                                                          gTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGc                                                                                        >  5:213699/1‑148 (MQ=255)
                                                           tttCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAACCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCa                                                                                      <  6:123467/149‑1 (MQ=255)
                                                                tGCCTGGCGGTACTTTGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGAtt                                                                                 >  5:59855/1‑149 (MQ=255)
                                                                            cTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGc                                                                      >  2:158129/1‑148 (MQ=255)
                                                                                 tCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAatat                                                                                                             <  8:224010/104‑1 (MQ=255)
                                                                                 tCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAatat                                                                                                             >  7:224010/1‑104 (MQ=255)
                                                                                   atacataATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTca                                                              <  8:9045/149‑1 (MQ=255)
                                                                                   atacataATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTc                                                               <  6:59855/148‑1 (MQ=255)
                                                                                                   ccGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCATAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTc                                              >  4:374712/1‑149 (MQ=255)
                                                                                                    cGAAGCCCCTGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGTGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTcc                                             <  6:23484/149‑1 (MQ=255)
                                                                                                     gAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCt                                                                                                      >  5:46609/1‑91 (MQ=255)
                                                                                                     gAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCt                                                                                                      <  6:46609/91‑1 (MQ=255)
                                                                                                           cATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGAtttttt                                                                             <  7:290633/110‑1 (MQ=255)
                                                                                                           cATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGAtttttt                                                                             >  8:290633/1‑110 (MQ=255)
                                                                                                           cATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTCACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGa                                       >  7:294538/1‑148 (MQ=255)
                                                                                                                 aCCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAAtttt                                >  6:299206/1‑149 (MQ=255)
                                                                                                                    gACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGGGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTaa                             <  2:107674/149‑1 (MQ=255)
                                                                                                                     aCAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTGAGCCAGGATGTTTTCGGTTGCAAGGTTGAGACAGGAATGTCTTTCCGCGCGATTATTTTTacc                            >  6:290412/1‑147 (MQ=255)
                                                                                                                       aaaaCTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGc                          >  5:150940/1‑149 (MQ=255)
                                                                                                                       aaaaCTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGc                          >  6:253091/1‑149 (MQ=255)
                                                                                                                       aaaaCTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGc                          >  7:424075/1‑149 (MQ=255)
                                                                                                                       aaaaCTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGc                          >  8:178396/1‑149 (MQ=255)
                                                                                                                        aaaCTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGcc                         >  3:35627/1‑149 (MQ=255)
                                                                                                                         aaCTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGccc                        >  1:263740/1‑149 (MQ=255)
                                                                                                                            ttttAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTg                     >  8:131251/1‑149 (MQ=255)
                                                                                                                            ttttAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACAAAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTg                     >  2:295532/1‑149 (MQ=255)
                                                                                                                                    ttACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAg             >  7:53064/1‑149 (MQ=255)
                                                                                                                                      aCTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAgg            <  3:366084/148‑1 (MQ=255)
                                                                                                                                      aCTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAgg            <  1:295532/148‑1 (MQ=255)
                                                                                                                                        tGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGt         >  4:100074/1‑149 (MQ=255)
                                                                                                                                        tGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGt         >  2:53445/1‑149 (MQ=255)
                                                                                                                                            aTCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCTTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTgc     <  6:14232/149‑1 (MQ=255)
                                                                                                                                            aTCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTgc     <  6:19845/149‑1 (MQ=255)
                                                                                                                                               ttCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCa  >  7:316490/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCA  >  NC_000913/1753622‑1753913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑