Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,722,202 T→G 100% D750E (GAT→GAG pka → protein lysine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,722,2020TG99.0% 365.4 / ‑4.2 102D750E (GAT→GAGpkaprotein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (46/55);  total (47/55)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 102 total aligned reads displayed.

GAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAC  >  NC_000913/2722056‑2722342
                                                                                                                                                  |                                                                                                                                            
gagaGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCt                                                                                                                                            <  1:284290/149‑1 (MQ=255)
gagaGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTACCCAACGAAGAGCt                                                                                                                                            >  1:83094/1‑149 (MQ=255)
gagaGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGGTCCGCCCGATTTTGCCAGAAGATGAGCCCCAACTTCAGCAATTCATTTCGCGAGTCACCGAAGAAGAGCt                                                                                                                                            >  7:159893/1‑149 (MQ=255)
  gagTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCttt                                                                                                                                          <  2:192839/149‑1 (MQ=255)
     tCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTttatt                                                                                                                                       <  1:559/149‑1 (MQ=255)
       ggCTGGCAGTGCGCCCTTATCCGCATCAGCTTGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTAc                                                                                                                                     <  2:254319/149‑1 (MQ=255)
             cAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTAc                                                                                                                               <  4:58187/149‑1 (MQ=255)
               gTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACtt                                                                                                                             >  1:419528/1‑149 (MQ=255)
                tGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACtt                                                                                                                             >  4:254331/1‑148 (MQ=255)
                   gcCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGc                                                                                                                         <  3:87040/149‑1 (MQ=255)
                   gcCCTTATCCGCATCAGCGGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCTGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGc                                                                                                                         <  3:97839/149‑1 (MQ=255)
                    cccTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGc                                                                                                                         >  8:418407/1‑148 (MQ=255)
                     ccTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGc                                                                                                                         >  7:386918/1‑147 (MQ=255)
                     ccTCATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCGACCACTTCAGCAATTCATGTCGCGAGTCCCCAAAGAAGATCTTTATTACCGCTACTTAAGc                                                                                                                         >  2:81318/1‑147 (MQ=255)
                         aTCCGCTTCAGCTGGTAGATTGGGTAGAATTGAAAAACGGTGATAGCTGCTTGTTCAGCCCGATTTTGCCAGATGATGAGCCTCTACTTCATCAATTGTTTTCGCGAGTCACCTAAGAAGAGCTTTATTACAGCTACTTTTGCgata                                                                                                                     <  8:177921/147‑3 (MQ=255)
                         aTCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCgaga                                                                                                                     <  5:206090/147‑1 (MQ=255)
                             gCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAAc                                                                                                                >  4:337761/1‑148 (MQ=255)
                               aTCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGaa                                                                                                             >  8:36065/1‑149 (MQ=255)
                                tCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAAt                                                                                                            >  7:54144/1‑149 (MQ=255)
                                  aGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAAtt                                                                                                           <  1:32993/148‑1 (MQ=255)
                                   gCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTa                                                                                                         <  1:406214/149‑1 (MQ=255)
                                    cTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTAc                                                                                                        <  7:36065/149‑1 (MQ=255)
                                     tGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAAtt                                                                                                           >  1:151901/1‑145 (MQ=255)
                                     tGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAAtt                                                                                                           <  2:151901/145‑1 (MQ=255)
                                      ggAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTAcc                                                                                                       >  7:92272/1‑148 (MQ=255)
                                       gaagaaTGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTAccc                                                                                                      <  2:95363/148‑1 (MQ=255)
                                       gaagaaTGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTAcc                                                                                                       <  3:254331/147‑1 (MQ=255)
                                       gaagaaTGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCa                                                                                                     <  7:418407/149‑1 (MQ=255)
                                          gaaTGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATTAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATg                                                                                                   >  6:362674/1‑148 (MQ=255)
                                           aaTGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGaa                                                                                                 <  5:362674/149‑1 (MQ=255)
                                              gggTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGAt                                                                                              >  3:312220/1‑149 (MQ=255)
                                                gTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGAtt                                                                                             >  3:179216/1‑148 (MQ=255)
                                                 tAGAATTGAAAAACGTTGAACGCTGCGTTTTCCGCCAGATTTTGACATAAGATGAGCCACAACTTCAGCAATTCATTTAGCGATTAACCAAAGAAGAGCTTTATTACCTCTACTTTATCGTTATCAACTAATTTACCCATGAAGATTTa                                                                                           <  3:321583/149‑1 (MQ=255)
                                                 tAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTa                                                                                           <  2:419528/149‑1 (MQ=255)
                                                   gAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACAAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGc                                                                                         <  5:189040/149‑1 (MQ=255)
                                                    aaTTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTGGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCTACGAATTTACCCATGAAGATTTAGc                                                                                         <  3:208456/148‑1 (MQ=255)
                                                     aTTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGcc                                                                                        >  1:305887/1‑148 (MQ=255)
                                                        gAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACa                                                                                    >  7:133543/1‑149 (MQ=255)
                                                        gAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGACAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACa                                                                                    >  7:133550/1‑149 (MQ=255)
                                                                     cGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGa                                                                       <  8:54144/149‑1 (MQ=255)
                                                                          ctTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTAc                                                                   >  8:338799/1‑148 (MQ=255)
                                                                             gTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATc                                                               >  5:268710/1‑149 (MQ=255)
                                                                              ttCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCg                                                              <  3:377721/149‑1 (MQ=255)
                                                                              ttCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCg                                                              <  2:305887/149‑1 (MQ=255)
                                                                                   cccGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGaaa                                                         >  3:305073/1‑149 (MQ=255)
                                                                                          ttGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGt                                                   <  6:268710/148‑1 (MQ=255)
                                                                                           tGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGtt                                                  <  3:82719/148‑1 (MQ=255)
                                                                                            gCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAACGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGttt                                                 <  5:379456/148‑1 (MQ=255)
                                                                                              cAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTg                                                >  2:431898/1‑147 (MQ=255)
                                                                                               agaagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAg                                             >  8:44719/1‑149 (MQ=255)
                                                                                                 aagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCg                                           <  4:305073/149‑1 (MQ=255)
                                                                                                 aagaTGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCg                                           >  8:242193/1‑149 (MQ=255)
                                                                                                     tGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAg                                             <  4:23468/143‑1 (MQ=255)
                                                                                                     tGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAg                                             >  3:23468/1‑143 (MQ=255)
                                                                                                     tGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTac                                       >  1:69787/1‑149 (MQ=255)
                                                                                                      gAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATTACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTac                                       >  1:424831/1‑148 (MQ=255)
                                                                                                      gAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTac                                       >  3:132454/1‑148 (MQ=255)
                                                                                                       aGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTacga                                     <  7:338799/149‑1 (MQ=255)
                                                                                                         ccACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGc                                                                                                                         <  7:425345/63‑1 (MQ=255)
                                                                                                         ccACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGc                                                                                                                         >  8:425345/1‑63 (MQ=255)
                                                                                                         ccACAACTTCACCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCCAGATCAACGAATATACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGAACGGGAAATGGCGTTTGTAGCGGTacgacg                                   >  5:122125/1‑149 (MQ=255)
                                                                                                          cacaACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTCTATTACCGCTACTTTAGc                                                                                                                         >  4:185731/1‑62 (MQ=255)
                                                                                                          cacaACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTCTATTACCGCTACTTTAGc                                                                                                                         <  3:185731/62‑1 (MQ=255)
                                                                                                            caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAAGGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGCTCGGGAAATGGCGTTTGTAGCGGTACGACGTAt                                <  2:55689/149‑1 (MQ=255)
                                                                                                            caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAt                                >  5:82365/1‑149 (MQ=255)
                                                                                                            caACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAt                                <  6:139316/149‑1 (MQ=255)
                                                                                                               cTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATAAACGAATTTACCCATGAAGATTTAGACAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGa                             >  7:374403/1‑149 (MQ=255)
                                                                                                                ttCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTacgac                                    <  5:235429/141‑1 (MQ=255)
                                                                                                                ttCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTacgac                                    >  6:235429/1‑141 (MQ=255)
                                                                                                                 tCAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATc                           <  8:133550/149‑1 (MQ=255)
                                                                                                                  cAGCAATTCATTTCGCGAGTCACCGAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGAt                            <  8:159893/147‑1 (MQ=255)
                                                                                                                  cAGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATc                           <  8:133543/148‑1 (MQ=255)
                                                                                                                  cAGCAATTCATTTCGCGAGTCACCAAACAAGAGCTTTATTACCGCTACTTTACCGAGATCATCGAATTTACCCATGAAGATTAAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGAt                            <  1:170992/147‑1 (MQ=255)
                                                                                                                   aGCAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCa                          >  7:186343/1‑148 (MQ=255)
                                                                                                                    gCAATTCATTTCGCTAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACTACGTATTGATCaaa                        <  8:428892/149‑1 (MQ=255)
                                                                                                                     cAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAAc                       >  7:176667/1‑149 (MQ=255)
                                                                                                                     cAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAAc                       <  4:132454/149‑1 (MQ=255)
                                                                                                                     cAATTCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAAc                       <  4:312220/149‑1 (MQ=255)
                                                                                                                      aaTTCATTTCGCGAGTCACCCAAGAAGAGCTTTCTTACCGCTCCTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATCACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTCTTGATCAAACg                      <  8:132463/149‑1 (MQ=255)
                                                                                                                        ttCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACgg                     >  8:394859/1‑148 (MQ=255)
                                                                                                                        ttCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGa                    <  1:431898/149‑1 (MQ=255)
                                                                                                                         tCATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACgg                     >  2:192094/1‑147 (MQ=255)
                                                                                                                          cATTTCGCGAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGaa                   <  8:186343/148‑1 (MQ=255)
                                                                                                                               cgcgAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGAt              <  1:192094/148‑1 (MQ=255)
                                                                                                                                gcgAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAt                                <  7:29189/129‑1 (MQ=255)
                                                                                                                                gcgAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAt                                >  8:29189/1‑129 (MQ=255)
                                                                                                                                  gAGTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTc          >  4:25831/1‑149 (MQ=255)
                                                                                                                                   aGTCACCAAAGAAGAGCTTTATTACCGATACTTTAGCGAGATCAACGAATTTTCCCATGAAGATTTAGCCAAGATGACAGTGTTCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCg         <  2:83094/149‑1 (MQ=255)
                                                                                                                                    gTCACCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCgg        >  2:402532/1‑149 (MQ=255)
                                                                                                                                       aCCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGAt                            <  4:1429/126‑1 (MQ=255)
                                                                                                                                       aCCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGAt                            >  3:1429/1‑126 (MQ=255)
                                                                                                                                       aCCAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGt     >  4:65978/1‑149 (MQ=255)
                                                                                                                                         cAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGTCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAg                                             >  3:16399/1‑107 (MQ=255)
                                                                                                                                         cAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGTCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAg                                             <  4:16399/107‑1 (MQ=255)
                                                                                                                                         cAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTc          <  5:422476/142‑1 (MQ=255)
                                                                                                                                         cAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTc          >  6:422476/1‑142 (MQ=255)
                                                                                                                                         cAAAGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCa   >  4:376368/1‑149 (MQ=255)
                                                                                                                                          aaaGAAGAGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAc  <  7:242193/149‑1 (MQ=255)
                                                                                                                                              aagaGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAtt                               <  8:321606/116‑1 (MQ=255)
                                                                                                                                              aagaGCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTAtt                               >  7:321606/1‑116 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
GAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCAC  >  NC_000913/2722056‑2722342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: