| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,260,401 | G→A | 5.9% | S673L (TCA→TTA) | tdcE ← | pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,260,401 | 0 | G | A | 5.9% | 381.8 / 8.6 | 119 | S673L (TCA→TTA) | tdcE | pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (61/51); new base A (5/2); total (66/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCG > NC_000913/3260254‑3260548 | tCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTga < 1:157797/149‑1 (MQ=255) agcagcATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTgag > 3:204815/1‑147 (MQ=255) gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGCCCGCCTTCGACCTCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAg < 6:220640/149‑1 (MQ=255) gcagcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAg < 8:173852/149‑1 (MQ=255) agcaTTTCCCGATTGATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGGAGGCCGACATGGTTTGTTTTACGTAGTGGATCTTCTTTGCCCAGCGCGTCAGGAACGATTGAGAAGgt < 4:373476/149‑1 (MQ=255) tttCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTTGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTAc < 7:61319/149‑1 (MQ=255) ttCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTAc > 4:371944/1‑148 (MQ=255) cccGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGGTGCGTGGTGGATATCCGCATCCAGGAGGCCGACTTGGTTGGGTTTACGTGCTGGAACTTCGTGGCCCAGCGGCGCCGGAGCGAGGGAGACTGTGGACggg > 4:38028/1‑147 (MQ=255) ccGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACCAGGGTGGGGTTAGGTACTGGATCTTCTTTGCCCGGCGCCGCAGGAACGATTGAGAAGGTGTACga > 8:227377/1‑147 (MQ=255) aTTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGGGTACGAGATCCCATc < 5:179163/149‑1 (MQ=255) aTTACGTTGACGGTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATc < 3:228719/149‑1 (MQ=255) cgttgacgttgaGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTg < 4:204815/149‑1 (MQ=255) ttGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGc < 6:229748/148‑1 (MQ=255) aCCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGtt > 3:184704/1‑149 (MQ=255) cGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTGTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTc > 1:418342/1‑149 (MQ=255) cTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGc > 3:45858/1‑127 (MQ=255) cTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGc < 4:45858/127‑1 (MQ=255) cTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGcc > 3:247865/1‑149 (MQ=255) cTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGc > 8:244543/1‑148 (MQ=255) aCATCCGCTTCGTGGTGTAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTCTACGAGATCCCATCTTTGGCGTAGGTGAACTGCAGTTTCGCCACCGa > 5:336197/1‑149 (MQ=255) aCATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGtt > 1:214473/1‑139 (MQ=255) aCATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGtt < 2:214473/139‑1 (MQ=255) tCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACga > 6:399352/1‑104 (MQ=255) tCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACga < 5:399352/104‑1 (MQ=255) tCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTg < 6:105440/117‑1 (MQ=255) tCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGt < 4:247865/149‑1 (MQ=255) tCCGCTTCGTAGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTg > 5:105440/1‑117 (MQ=255) cGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGGTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGGGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGCCGTc > 4:294900/1‑148 (MQ=255) cTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAAt < 6:188884/149‑1 (MQ=255) cTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAAt > 5:131706/1‑149 (MQ=255) cGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGa < 7:244543/148‑1 (MQ=255) gtggtgGAAATACCCATCGAGCAGGCCGACAAGGGTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAg < 2:418342/148‑1 (MQ=255) tggtggAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc > 3:280220/1‑149 (MQ=255) tggtggAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc > 4:392873/1‑149 (MQ=255) gtggAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCa > 2:247829/1‑149 (MQ=255) gtggAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCa < 5:217957/149‑1 (MQ=255) aaTACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCa > 4:129093/1‑149 (MQ=255) aCCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCt > 8:116400/1‑149 (MQ=255) cgcATCCAGCAGGCCGTCAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCATGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGTACGGCAGTTTCGCCACCGACGTCAATGAGGGCACGGCACCt < 4:287986/146‑1 (MQ=255) cccATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTg > 2:82112/1‑97 (MQ=255) ccATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTg > 8:33783/1‑96 (MQ=255) ccATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTc > 3:255599/1‑117 (MQ=255) ccATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTc < 4:255599/117‑1 (MQ=255) ccATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGCGGCCACGGCACCttt > 2:305388/1‑149 (MQ=255) ccATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCttt < 7:103233/149‑1 (MQ=255) ccATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCttt > 4:220218/1‑149 (MQ=255) cATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGGCCAGCGCCGCAGGAACGATTGAGAAGGTGTAAGAGCTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTg < 7:440428/149‑1 (MQ=255) cATCCAGCAGGCCGACAAGGTTGGGTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTg < 3:295056/149‑1 (MQ=255) aTCGAGCAGGCTGACAATGTTGGTTTTTGGTACTTGATCTTCTTTGCCCAGCGGCTCAGGAACTTTTGAGAAGGTTTACGAGATCCCATATTTGGCGTAGGTGAACGGAAGTTTCGCCACCGACGTGAATGAGGCCACGGCACCTTTGc < 6:336197/149‑1 (MQ=255) aTCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAg < 7:326373/133‑1 (MQ=255) aTCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGc > 7:98568/1‑149 (MQ=255) ccAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGa > 4:293981/1‑130 (MQ=255) ccAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGa < 3:293981/130‑1 (MQ=255) cagcagGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCgg < 7:116400/148‑1 (MQ=255) agcagGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCCCCGACGTCAATGAGGCCACGGCACCTTTGCGGTc > 1:101803/1‑149 (MQ=255) gcagGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGCACGATTGAGAAGGTGTACGAGCTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCa > 1:72313/1‑149 (MQ=255) cagGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCAc > 4:190211/1‑149 (MQ=255) agGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGt < 5:375839/95‑1 (MQ=255) agGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGt > 6:375839/1‑95 (MQ=255) aCAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCt > 3:231757/1‑78 (MQ=255) aCAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCt < 4:231757/78‑1 (MQ=255) ggttggttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAAt > 8:326940/1‑113 (MQ=255) ggttggttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAAt < 7:326940/113‑1 (MQ=255) ggttggttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATc < 7:397895/149‑1 (MQ=255) gttggttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCg > 2:281822/1‑149 (MQ=255) gttggttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCg > 5:238689/1‑149 (MQ=255) gttggttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCg > 2:42785/1‑149 (MQ=255) gttggttTTACGTACTGGATCTTC‑TTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCgg > 4:348149/1‑149 (MQ=255) gttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCa > 6:114610/1‑88 (MQ=255) gttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCa > 4:6465/1‑88 (MQ=255) gttTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCa < 3:6465/88‑1 (MQ=255) ttttACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGt > 6:66566/1‑147 (MQ=255) aCTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCgg > 6:385400/1‑149 (MQ=255) tGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTa > 3:233850/1‑139 (MQ=255) gATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGa > 4:298965/1‑122 (MQ=255) gATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGgcgc > 5:160795/1‑148 (MQ=255) gATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGgcgc < 2:101803/148‑1 (MQ=255) gATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACgaga < 2:78462/48‑1 (MQ=255) gATCTTCTTTGCCCAGCGCCGCAGGAACGATTAAGAAGGTGTACgaga > 1:78462/1‑48 (MQ=255) tcttctTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGgcgc > 6:400653/1‑146 (MQ=255) cttcttTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAg > 4:273643/1‑95 (MQ=255) cttcttTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAg < 3:273643/95‑1 (MQ=255) tcttTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACgg > 7:129376/1‑149 (MQ=255) cttTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAAc < 1:305388/146‑1 (MQ=255) tttGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGgtg > 8:333922/1‑149 (MQ=255) tGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGgt < 6:160795/146‑1 (MQ=255) cccAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTc < 3:190211/148‑1 (MQ=255) ccAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCg > 1:60716/1‑149 (MQ=255) ccAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGACACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCg > 8:203424/1‑149 (MQ=255) cAGCGCCGCAGGAACGATTAAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCgg > 8:437488/1‑149 (MQ=255) gcgcCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGGAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGgcg < 1:281822/149‑1 (MQ=255) gcgcCGCAGGAACGAGTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGgcg < 7:227377/149‑1 (MQ=255) gAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGgtgt < 4:270287/133‑1 (MQ=255) aaCGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGgc > 4:18096/1‑121 (MQ=255) aaCGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGgc < 3:18096/121‑1 (MQ=255) aTTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAg > 4:373428/1‑69 (MQ=255) aTTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAg < 3:373428/69‑1 (MQ=255) ttGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGc < 7:128444/149‑1 (MQ=255) ttGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGc > 5:95798/1‑149 (MQ=255) tGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCg > 5:15076/1‑149 (MQ=255) | TCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCG > NC_000913/3260254‑3260548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |