Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,159,199 | G→T | G444V (GGC→GTC) | ptsG → | fused glucose‑specific PTS enzymes: IIB component/IIC component |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,159,199 | 0 | G | T | 98.0% | 335.4 / NA | 99 | G444V (GGC→GTC) | ptsG | fused glucose‑specific PTS enzymes: IIB component/IIC component |
Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (50/47); total (50/49) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.42e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 101 total aligned reads displayed.
CAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTT > NC_000913/1159055‑1159347 | cAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGtcg > 1:252737/1‑147 (MQ=255) tACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCgg > 3:310912/1‑149 (MQ=255) ccAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGc < 7:63172/149‑1 (MQ=255) gCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCg > 5:50302/1‑147 (MQ=255) cGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAg < 3:278142/149‑1 (MQ=255) gAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAACCTGGTCGCAGCGGGCGTAGt > 1:263055/1‑149 (MQ=255) aTGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGt < 4:5530/149‑1 (MQ=255) tGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGtt < 2:252737/149‑1 (MQ=255) gCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGc < 8:47522/143‑1 (MQ=255) gCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGc > 7:47522/1‑143 (MQ=255) tctGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg > 3:130049/1‑149 (MQ=255) tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTACGCTCGACGCAGGTATTACCCGGCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg < 5:186735/147‑1 (MQ=255) tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg < 4:449523/147‑1 (MQ=255) tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg > 8:328510/1‑147 (MQ=255) tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg < 4:310912/147‑1 (MQ=255) tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg < 7:328510/147‑1 (MQ=255) ttGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGtt < 3:91260/149‑1 (MQ=255) gctgcATTAGGTGGTAAAGAAAACATTACTAACCTCGACGCATGAATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTAGCTGGTTCTGGTGtt > 8:117472/1‑147 (MQ=255) ctgcATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCa < 2:242925/148‑1 (MQ=255) gcATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGc > 6:411186/1‑149 (MQ=255) gtggtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGAtttt < 6:282812/148‑1 (MQ=255) tggtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg < 6:50302/149‑1 (MQ=255) ggtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCgg > 4:81602/1‑149 (MQ=255) gtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCgg > 2:273290/1‑148 (MQ=255) gtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCgg > 2:438230/1‑148 (MQ=255) gtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCgg > 6:242442/1‑148 (MQ=255) tAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTa < 4:130049/149‑1 (MQ=255) aaGAAAACATTAATAACATCGACGCATGTATTAACCGTCTGCGCGTCAGCGTTGCAGATTAGTCTAAATTGTATAAGTACGGCCTGAAGAAAATGTTCGCAGCTGTCTAAGTGGTTACTTGTTCTGGTCTTCAGTCGATTTTCGGTAc > 5:291123/1‑148 (MQ=255) aGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACt > 3:179102/1‑148 (MQ=255) aGAAAACATTACTAACCTCGACGCATGTATTACACGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACt > 8:460369/1‑148 (MQ=255) aaaaCATTTCTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGATGATGTGTCTAAAGGGGATCAGGCCGGGCTGAAGAAACTGGTCTCAGCGGGCGTAGTGGTTGCTGGTGCTGGTGTTGAGGCGATTTTCGGTACTaaa < 5:233410/149‑1 (MQ=255) aaCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGATGATGTGTCTAAAGTGTATCAGGCCGGCCTGAAGAAACTGGTCTCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATc < 6:195003/149‑1 (MQ=255) cTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg > 8:243204/1‑132 (MQ=255) cTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg < 7:243204/132‑1 (MQ=255) cTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAAc < 4:400220/149‑1 (MQ=255) aCCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCt > 2:487642/1‑148 (MQ=255) aCCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCt < 5:242442/148‑1 (MQ=255) ccTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCTGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTg > 8:208759/1‑148 (MQ=255) ctctACCCATGTAAAACCCGTCAGCGCGTCAGCTTAGGAGATGTTTCTAAAGTGGATCAGGCCGGCCTCAAGAAACTGCTCGCAGCGGGCGTAGAGGTTGCTGGTTCTCGTGAACAGGCGATATACGGTACAAAAACCTAAAAACTGa > 4:411903/1‑148 (MQ=255) tCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGt < 3:194416/94‑1 (MQ=255) tCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGt > 4:194416/1‑94 (MQ=255) tCGACCCATGTATTAACCGTCTGCGCGTCAGCGATGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGATGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATACGATAACCTGaa > 2:368671/1‑148 (MQ=255) aCGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGGTCTGGTGTTCAGGCGATTTTCGGTAATAAATCCGATAACCTGAAAAcc < 2:470415/149‑1 (MQ=255) cGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCg < 3:232228/149‑1 (MQ=255) gCATGTATTACCCGTCTGCGCGTCAGCGTTGCCGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCga < 8:28576/149‑1 (MQ=255) aTGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAAcc > 5:320856/1‑145 (MQ=255) tGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGa > 3:106491/1‑131 (MQ=255) tGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGa < 4:106491/131‑1 (MQ=255) aTTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCTCAGCGGGCGTAGTGGTTGCTGGTTATGGTGTTCAGGCGATTTTCGGTAGTAAATCCGATAACCTGAAAACCGAgatgga > 5:76477/1‑149 (MQ=255) aTTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTATTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATACGAAAACCTGAAAACCGAgatgga > 2:374472/1‑149 (MQ=255) ttACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat < 1:25286/149‑1 (MQ=255) cccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga > 4:80405/1‑141 (MQ=255) cccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga < 3:80405/141‑1 (MQ=255) ccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAg > 1:60221/1‑148 (MQ=255) ccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGt < 2:60221/149‑1 (MQ=255) cGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGAt < 8:282995/123‑1 (MQ=255) cGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGAt > 7:282995/1‑123 (MQ=255) gTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAc < 7:460369/149‑1 (MQ=255) cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACAt < 4:179102/146‑1 (MQ=255) gTCAGCGTTGCTGAGGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCTGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTaa < 7:289530/149‑1 (MQ=255) tCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAAc < 6:320856/149‑1 (MQ=255) aGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCa < 5:411186/149‑1 (MQ=255) aTGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGt > 4:158234/1‑148 (MQ=255) tgtgTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTaa < 1:438230/149‑1 (MQ=255) tgtCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGa > 8:422603/1‑149 (MQ=255) tCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCCGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACg > 6:463110/1‑149 (MQ=255) tCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCTTAAGACg > 8:273067/1‑149 (MQ=255) cTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGt > 8:440625/1‑149 (MQ=255) tAAAGTGGATCAGGCCTGCCTGAAGAAACTTGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat < 5:55572/115‑1 (MQ=255) tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATcc > 6:29435/1‑91 (MQ=255) tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATcc < 5:29435/91‑1 (MQ=255) tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat > 6:55572/1‑115 (MQ=255) tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGtt > 1:49211/1‑149 (MQ=255) tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGtt > 4:108317/1‑149 (MQ=255) aaGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCTGTAACCACTAATCCGTAAGACGtt < 7:117472/147‑1 (MQ=255) aaGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGt < 2:261140/139‑1 (MQ=255) aaGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGt > 1:261140/1‑139 (MQ=255) aGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTggg < 7:422603/149‑1 (MQ=255) aGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACC‑CTAATCCg < 8:370859/136‑1 (MQ=255) aGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACC‑CTAATCCg > 7:370859/1‑136 (MQ=255) ggggATCAGGCCGGCCTGAAGAAACTGGTCCCAGCGGGCGTATGGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTgggg < 7:102905/147‑1 (MQ=255) gTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGTTGTTCAGGCGATTTTCGGTACTAAATCCTATAACGGGAACACCGAGATGGATGAATCCATCCGTAACCAAGAATCCGTAAGACgggggg > 8:353350/1‑143 (MQ=255) gTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTaa > 2:508027/1‑139 (MQ=255) gTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTaa < 1:508027/139‑1 (MQ=255) gTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTggg > 3:456126/1‑148 (MQ=255) aTCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGAc > 2:345965/1‑149 (MQ=255) tCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGAc > 7:234822/1‑148 (MQ=255) tCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGAc > 2:402421/1‑148 (MQ=255) cAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACt > 1:231498/1‑148 (MQ=255) ggccggccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGgatcggaag > 7:33224/1‑142 (MQ=255) agccggccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGGGTTCAGGCTATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTg < 8:33224/137‑1 (MQ=255) ccggccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGaaa < 6:457325/89‑1 (MQ=255) tcTGATGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGGTCTGGTGTTCAGGCGATTTTCGGTACTAATTCCGATAACCTGAAATCCGAGATGGATGAGTACATCCGTAACCACTAATCGGTAAGACGTTGGGGAGACTAAGGCAGcc < 3:411903/148‑1 (MQ=255) ccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga > 2:264857/1‑92 (MQ=255) ccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga < 1:264857/92‑1 (MQ=255) gaaACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCatctgg < 1:273290/149‑6 (MQ=255) gaaACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATgg < 3:158234/149‑1 (MQ=255) cTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgat < 2:349342/83‑1 (MQ=255) cTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgat > 1:349342/1‑83 (MQ=255) gcgcAGCGGGCGTAGTGGTTGCGGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCtta < 3:81602/149‑2 (MQ=255) | CAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTT > NC_000913/1159055‑1159347 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |