New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2700550 =	31 (1.070)	5 (0.200)	5/214	2.6	17.8%	noncoding (31/77 nt)	ohsC	sRNA antisense regulator of shoB toxin
	?	NC_000913	2700601 =	20 (0.820)					intergenic (+5/+17)	ohsC/acpS	sRNA antisense regulator of shoB toxin/holo‑[acyl‑carrier‑protein] synthase 1
Rejected: Frequency below/above cutoff threshold.

GCGGTCACGTTAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACAT  >  NC_000913/2700406‑2700676                                                                                                                                                 |                                                                                                                               gCGGTCACGTTAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTaaa                                                                                                                            <  7:46259/149‑1 (MQ=255)    accacggctacggctatggggcgaggcattaaaactggcggaaaagctgggcgttgcaaatatgcatgtaacgctggcagatgagcggcactatgcttgtgccacggtaattattgaaagttaaaaataataccgttctctTTAAAAAg                                                                                                                         >  2:125339‑M1/142‑149 (MQ=255)         gTTAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTaaaa                                                                                                                    >  2:108040/1‑149 (MQ=255)         gTTAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCGGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTaaaa                                                                                                                    >  2:110311/1‑149 (MQ=255)                     aTCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCt                                                                                                         >  1:25721/1‑148 (MQ=255)                       cAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAGGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTAc                                                                                                      >  7:11460/1‑149 (MQ=255)                              gCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCg                                                                                               >  2:148023/1‑149 (MQ=255)                               attaaaactggcggaaaagctgggcgttgcaaatatgcatgtaacgctggcagatgagcggcactatgcttgtgccacggtaattattgaaagttaaaaataataccgttctctTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCgg                                                                                              >  8:57804‑M1/115‑149 (MQ=255)                                  tcttcGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCgggg                                                                                            <  2:25721/148‑1 (MQ=255)                                   aaactggcggaaaagctgggcgttgcaaatatgcatgtaacgctggcagatgagcggcactatgcttgtgccacggtaattattgaaagttaaaaataataccgttctctTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCggggg                                                                                           >  6:97001‑M1/111‑148 (MQ=255)                                          aaGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTaaaa                                                                                                                    <  7:29339/116‑1 (MQ=255)                                          aaGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTaaaa                                                                                                                    >  8:29339/1‑116 (MQ=255)                                                 gctgggcgttgcaaatatgcatgtaacgctggcagatgagcggcactatgcttgtgccacggtaattattgaaagttaaaaataataccgttctctTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAag                                                                            >  6:65234‑M1/97‑149 (MQ=255)                                                        tATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACg                                                                      <  1:148023/148‑1 (MQ=255)                                                           gCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTaa                                                                                                                             <  6:72192/90‑1 (MQ=255)                                                           gCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTaa                                                                                                                             >  5:72192/1‑90 (MQ=255)                                                                        aGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACttt                                                     <  1:110311/149‑1 (MQ=255)                                                                        aGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACttt                                                     <  1:108040/149‑1 (MQ=255)                                                                                    aaGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGATGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCg                                         >  4:59372/1‑149 (MQ=255)                                                                                      gATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTg                                       <  4:117694/149‑1 (MQ=255)                                                                                        tAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTgg                                      <  3:59372/148‑1 (MQ=255)                                                                                                 tGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGt                                        >  3:96479/1‑137 (MQ=255)                                                                                                 tGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGt                                        <  4:96479/137‑1 (MQ=255)                                                                                                    ttgtgccacggtaattattgaaagttaaaaataataccgttctctTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTAc                                                                                     <  1:31726‑M1/44‑1 (MQ=255)                                                                                                    ttgtgccacggtaattattgaaagttaaaaataataccgttctctTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTAc                                                                                     >  2:31726‑M1/46‑89 (MQ=255)                                                                                                    tCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTg                         >  6:38468/1‑149 (MQ=255)                                                                                                      cATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGc                        <  8:11460/148‑1 (MQ=255)                                                                                                         aaTCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACttttt                                                                                <  8:57770/89‑1 (MQ=255)                                                                                                         aaTCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACttttt                                                                                >  7:57770/1‑89 (MQ=255)                                                                                                           tCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCaa                                                  <  2:93639/117‑1 (MQ=255)                                                                                                           tCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCaa                                                  >  1:93639/1‑117 (MQ=255)                                                                                                               tgttgtTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTg              >  3:21072/1‑149 (MQ=255)                                                                                                                   gttgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCa           <  5:38468/148‑1 (MQ=255)                                                                                                                                    cacaAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGcc            >  6:142788/1‑130 (MQ=255)                                                                                                                                    cacaAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGcc            <  5:142788/130‑1 (MQ=255)                                                                                                                                    cacaAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACAt  <  6:65808/140‑1 (MQ=255)                                                                                                                                    cacaAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACAt  >  5:65808/1‑140 (MQ=255)                                                                                                                                                 TTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTaaa                                                                             <  2:149032‑M1/52‑1 (MQ=255)                                                                                                                                                 TTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTaaa                                                                             >  1:149032‑M1/1‑52 (MQ=255)                                                                                                                                                 |                                                                                                                               GCGGTCACGTTAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACAT  >  NC_000913/2700406‑2700676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.