Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	2,722,123	Δ7 bp	coding (2171‑2177/2661 nt)	pka →	protein lysine acetyltransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2722122	2 (0.070)	30 (1.060)	28/248	0.2	93.8%	coding (2170/2661 nt)	pka	protein lysine acetyltransferase
	?	NC_000913	2722130 =	2 (0.070)					coding (2178/2661 nt)	pka	protein lysine acetyltransferase

TCATCCTTTGCTGGCTTCTGGCAGTGAATTTACCGCGCTGGATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCC  >  NC_000913/2721977‑2722256                                                                                                                                                  |                                                                                                                                       tCATCCTTTGCTGGCTTCTGGCAGTGAATTTACCGCGCTGGATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGctt                                                                                                                                     >  8:29892‑M1/1‑146 (MQ=255) tCATCCTTTGCTGGCTTCTGGCAGTGAATTTACCGCGCTGGATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGctt                                                                                                                                     >  2:130351‑M1/1‑146 (MQ=255)      cTTTGCTGGCTTCTGGCAGTGAATTTACCGCGCTGGATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttcc                                                                                                                                >  6:52834‑M1/1‑141 (MQ=255)                     gCAGTGAATTTACCGCGCTGGATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgcc                                                                                                                   <  1:130351‑M1/147‑22 (MQ=255)                       aGTGAATTTACCGCGCTGGATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaa                                                                                                               <  8:18199‑M1/149‑26 (MQ=255)                            aTTTACCGCGCTGGATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagat                                                                                                            >  2:45354‑M1/1‑119 (MQ=255)                                   gcgcTGGATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaa                                                                                                   <  5:52834‑M1/149‑38 (MQ=255)                                         gATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttca                                                                                              <  1:45354‑M1/148‑43 (MQ=255)                                           tGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagc                                                                                            >  2:379‑M1/1‑104 (MQ=255)                                              cACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttca                                                                                              <  8:101957‑M1/143‑43 (MQ=255)                                              cACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttca                                                                                              >  7:101957‑M1/1‑101 (MQ=255)                                               aCGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaatt                                                                                        >  2:56359‑M1/1‑100 (MQ=255)                                                          ctcGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccac                                                                                                     <  4:70060‑M1/124‑36 (MQ=255)                                                          ctcGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccac                                                                                                     >  3:70060‑M1/1‑89 (MQ=255)                                                                     aaGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGTGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaa                                                                  <  7:29892‑M1/148‑71 (MQ=255)                                                                      aGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcattt                                                                                   >  1:36567‑M1/1‑77 (MQ=255)                                                                      aGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcattt                                                                                   <  2:36567‑M1/130‑54 (MQ=255)                                                                                            gCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagc                                          <  1:379‑M1/149‑95 (MQ=255)                                                                                                            cGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaattta                          >  3:18704‑M1/1‑39 (MQ=255)                                                                                                                tCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaatttac                         <  2:89018‑M1/146‑112 (MQ=255)                                                                                                                tCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaatttac                         >  1:89018‑M1/1‑35 (MQ=255)                                                                                                                tCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAAt                      >  4:30165/1‑149 (MQ=255)                                                                                                                tCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAAt                      >  6:14507/1‑149 (MQ=255)                                                                                                                     tGGAAGAATGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattac                                                      >  3:27494‑M1/1‑30 (MQ=255)                                                                                                                     tGGAAGAATGGGTAGAATTGAAAAACGGTGcttgtcccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattac                                                      <  4:27494‑M1/112‑83 (MQ=255)                                                                                                                          gaaTGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaatttacccatgaagatt              >  2:60136‑M1/1‑25 (MQ=255)                                                                                                                          gaaTGGGTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaatttacccatgaagatt              <  1:60136‑M1/147‑123 (MQ=255)                                                                                                                                gTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaatttacccatgaagatttagc          >  2:89149‑M1/1‑19 (MQ=255)                                                                                                                                gTAGAATTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaatttacccatgaagatttagc          <  1:89149‑M1/145‑127 (MQ=255)                                                                                                                                     aTTGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaatttacccatgaagatttagccaacatga  >  5:46604‑M1/1‑14 (MQ=255)                                                                                                                                      ttGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaatttaccca                      <  3:926‑M1/127‑115 (MQ=255)                                                                                                                                      ttGAAAAACGGTGcttgttccgcccgattttgccagaagatgagccacaacttcagcaattcatttcgcgagtcaccaaagaagatctttattaccgctactttagcgagatcaacgaatttaccca                      >  4:926‑M1/1‑13 (MQ=255)                                                                                                                                                  |                                                                                                                                       TCATCCTTTGCTGGCTTCTGGCAGTGAATTTACCGCGCTGGATGTCACGCTGGATATCTCGCCGTTTGAAGGCGATAACGAGAGTCGGCTGGCAGTGCGCCCTTATCCGCATCAGCTGGAAGAATGGGTAGAATTGAAAAACGGTGAACGCTGCTTGTTCCGCCCGATTTTGCCAGAAGATGAGCCACAACTTCAGCAATTCATTTCGCGAGTCACCAAAGAAGATCTTTATTACCGCTACTTTAGCGAGATCAACGAATTTACCCATGAAGATTTAGCC  >  NC_000913/2721977‑2722256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.