Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 911,086 IS2 (–) +5 bp intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)29 (1.010) 26/252 0.3 87.4% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 911086 = 4 (0.140)intergenic (‑37/+96) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
* ? NC_000913 = 382590NA (NA)22 (0.770) 20/250 0.6 84.1% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 911090 4 (0.150)intergenic (‑41/+92) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

GACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTC  >  NC_000913/910944‑911238
                                                                                                                                                  |                                                                                                                                                    
gACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGGTTCACCCCTGCCGAGTCGCGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGTTTCTCCATCTCCTGAATGTGATCAAGGTAACAAGTTTAGtg                                                                                                                                                    <  3:89091‑M2/149‑3 (MQ=255)
       aCTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgc                                                                                                                                             <  5:19771‑M2/149‑10 (MQ=255)
        cTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcc                                                                                                                                            <  7:110759‑M2/149‑11 (MQ=255)
                cgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAgggg                                                                                                                                     >  2:44878‑M2/127‑148 (MQ=255)
                            tccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtaataagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGCGGGAAGGGATgg                                                                                                                           >  6:92984‑M2/115‑146 (MQ=255)
                                       cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcactta                                                                                                              >  8:110759‑M2/1‑108 (MQ=255)
                                       cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgccc                                                                                                                                           >  4:43708‑M2/1‑108 (MQ=255)
                                       cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgccc                                                                                                                                           <  3:43708‑M2/119‑12 (MQ=255)
                                         cAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatct                                                                                                                         <  7:908‑M2/135‑30 (MQ=255)
                                         cAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatct                                                                                                                         >  8:908‑M2/1‑106 (MQ=255)
                                           gATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGAt                                                                                                                             >  4:24897/1‑129 (MQ=255)
                                           gATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGAt                                                                                                                             <  3:24897/129‑1 (MQ=255)
                                            aTGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccat                                                                                                        >  6:19771‑M2/1‑103 (MQ=255)
                                                  tctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGtt                                                                                                   >  1:10899‑M2/93‑148 (MQ=255)
                                                      ccccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctg                                                                                                                        <  8:61638‑M2/123‑31 (MQ=255)
                                                      ccccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctg                                                                                                                        >  7:61638‑M2/1‑93 (MQ=255)
                                                        ctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGc                                                                                            >  2:24703‑M2/87‑149 (MQ=255)
                                                        ccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCAGGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGgggatttgcccctatatttccagccatctgttagcactgaacccattacaagcccgct                                                                                            <  8:85280‑M2/149‑59 (MQ=255)
                                                         cTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctg                                                                                           <  6:18487‑M2/149‑60 (MQ=255)
                                                                 ctagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggcgccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGaaa                                                                                   <  2:10899‑M2/72‑1 (MQ=255)
                                                                   gAGTGGTTTGGCGATATAAGATGCAACCGTTTCTTGCATGGCTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttctcacttaacccattacaagcccgctgccgcagata                                                                                  >  4:89091‑M2/1‑80 (MQ=255)
                                                                        aaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAggg                                                                                                                               >  1:157032‑M2/71‑98 (MQ=255)
                                                                        aaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAggg                                                                                                                               <  2:157032‑M2/28‑1 (MQ=255)
                                                                         aaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGCCGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATatc                                                                           <  2:91442‑M2/80‑1 (MQ=255)
                                                                           tGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtg                                                                          >  5:18487‑M2/1‑72 (MQ=255)
                                                                              tagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAggg                                                                                                                                      <  3:75763‑M2/21‑1 (MQ=255)
                                                                               gATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccatta                                                                                                      >  6:122919‑M2/1‑68 (MQ=255)
                                                                                   ttaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCAt                                                                  >  1:54237‑M2/60‑148 (MQ=255)
                                                                                           aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTaaa                                                                                                >  1:70619/1‑110 (MQ=255)
                                                                                           aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTaaa                                                                                                <  2:70619/110‑1 (MQ=255)
                                                                                              acttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGgaga                                                                                                                       <  5:111082‑M2/36‑1 (MQ=255)
                                                                                              acttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGgaga                                                                                                                       >  6:111082‑M2/49‑84 (MQ=255)
                                                                                              acttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATg                                                      >  1:42636‑M2/49‑149 (MQ=255)
                                                                                                  atttaagtgatattggttgtctggagactcaggggcccagtctaTTTAGATCATCTGACGGAGGCGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCga                                                   <  1:44878‑M2/104‑1 (MQ=255)
                                                                                                        gtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGatgatat                                                                                  <  4:45937‑M2/73‑4 (MQ=255)
                                                                                                        gtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGatgatat                                                                                  >  3:45937‑M2/39‑108 (MQ=255)
                                                                                                            gtctccATCTCCTGAATGTTATCACGGTAACAAGTTTAGtggatgtgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgacgcagatattcacgtggcgagcgataacccagcgcactatgcggatgcca                                        >  4:29012‑M2/2‑39 (MQ=255)
                                                                                                                  ttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGc                                  >  3:68975‑M2/29‑149 (MQ=255)
                                                                                                                     tctggcgattcagggggccagtctaTTTACCTCAGCTGACGGAGCGCGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGa                               <  4:68975‑M2/124‑1 (MQ=255)
                                                                                                                       tggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATg                             >  7:115374‑M2/24‑149 (MQ=255)
                                                                                                                        ggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATgg                            >  1:39084‑M2/23‑149 (MQ=255)
                                                                                                                         gagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATGGCCCGATGAAAg                                                                                  >  1:15647‑M2/22‑94 (MQ=255)
                                                                                                                         gagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATg                             <  2:42636‑M2/126‑1 (MQ=255)
                                                                                                                         gAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgc                           <  5:79573‑M2/149‑124 (MQ=255)
                                                                                                                                   gggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc                 <  8:115374‑M2/138‑1 (MQ=255)
                                                                                                                                   gggccacgctaTTTACTTCATCTGCCCGAGGGGGACGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc                 <  2:39084‑M2/138‑1 (MQ=255)
                                                                                                                                     ggAAACAAGTTTAGtggatgtgcccctatatttccagacatctgttctcacttaacccattccaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctct               >  7:85280‑M2/1‑14 (MQ=255)
                                                                                                                                       cagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAg                   >  4:71561‑M2/8‑143 (MQ=255)
                                                                                                                                       cagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAg                   <  3:71561‑M2/136‑1 (MQ=255)
                                                                                                                                         gtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTAc           >  3:17762‑M2/6‑149 (MQ=255)
                                                                                                                                          cAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaag          <  4:125261‑M2/149‑141 (MQ=255)
                                                                                                                                                  gtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcg                                    <  7:99208‑M2/115‑115 (MQ=255)
                                                                                                                                                  gtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcg                                    >  8:99208‑M2/1‑1 (MQ=255)
                                                                                                                                                  gTTCATCTGTCTGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGTGCTTAAGGCGATTATGAGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTc  <  5:92984/149‑1 (MQ=255)
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GACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTC  >  NC_000913/910944‑911238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.