| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 2258692 = | 28 (0.970) | 6 (0.210) | 6/244 | 2.6 | 18.7% | coding (605/942 nt) | psuK | pseudouridine kinase |
| ? | NC_000913 | 2258736 = | 25 (0.890) | coding (561/942 nt) | psuK | pseudouridine kinase | |||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
ACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGGTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGTGCCTCTTC > NC_000913/2258589‑2258861 | aCCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGGTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCgg < 5:122475/148‑1 (MQ=255) gataatgccgccaacgtacccgtatttgtcgatccggtttcggcatggaaaggtgtcaaagtgcgcgaccggctaaatcagatccacactctcaagccaaacCGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGtt > 7:31143‑M1/103‑149 (MQ=255) atatCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGGTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCtt < 6:83365/149‑1 (MQ=255) atatCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGGTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCtt < 6:118646/149‑1 (MQ=255) taaACGCCGTCGCCGCCCATGCTCAATACCAGTCGGTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGAt < 4:122234/149‑1 (MQ=255) atccggtttcggcatggaaatgtgtcaaagtgcgcgaccgtctaaatcagatccacactctcaagccaaacCGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCgg > 3:108051‑M1/72‑116 (MQ=255) atccggtttcggcatggaaatgtgtcaaagtgcgcgaccgtctaaatcagatccacactctcaagccaaacCGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCgg < 4:108051‑M1/45‑1 (MQ=255) ggaaatgtgtcaaagtgcgcgaccgtctaaatcagatccacactctcaagccaaacCGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGttt < 1:113587‑M1/48‑1 (MQ=255) ggaaatgtgtcaaagtgcgcgaccgtctaaatcagatccacactctcaagccaaacCGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGttt > 2:113587‑M1/57‑104 (MQ=255) gTCGGTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACAt > 6:70350/1‑149 (MQ=255) ggTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTc < 3:122102/149‑1 (MQ=255) tgaaaTGTTGATGGAACCAGGAAGAAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCGTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCACGCACTTTGACACATTTCCATGc < 4:66234/144‑1 (MQ=255) ggCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCGTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGCTTTAGACGGTCACGCACTTTGACACATTTCCATGc > 3:66234/1‑148 (MQ=255) gATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGttt > 8:79124/1‑83 (MQ=255) gATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGttt < 7:79124/83‑1 (MQ=255) gATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGa > 1:150722/1‑149 (MQ=255) gcagcaACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACAtt > 6:45571/1‑121 (MQ=255) gcagcaACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACAtt < 5:45571/121‑1 (MQ=255) gcaACTTTTGCCACATCTTCACGCCCTGCCAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACggg > 8:62012/1‑149 (MQ=255) aCTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGAt < 5:105408/133‑1 (MQ=255) aCTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGAt > 6:105408/1‑133 (MQ=255) cacaTCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTc > 5:117596/1‑90 (MQ=255) cacaTCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTc < 6:117596/90‑1 (MQ=255) aaaccgccttgaagcggaaaccctgagtgggattgcgctgtcagggcgGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTcc < 1:113587‑M2/56‑1 (MQ=255) aaaccgccttgaagcggaaaccctgagtgggattgcgctgtcagggcgGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTcc > 2:113587‑M2/49‑104 (MQ=255) aaccgccttgaagcggaaaccctgagtgggattgcgctgtcagggcgGTTTGGCTTGAGAGTGTGGATCTGATTTAGCCGGTCGCGCACTTTGACACCTTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATc > 7:31143‑M2/48‑149 (MQ=255) ccgccttgaagcggaaaccctgagtgggattgcgctgtcagggcgGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGAt < 4:108051‑M2/71‑1 (MQ=255) ccgccttgaagcggaaaccctgagtgggattgcgctgtcagggcgGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGAt > 3:108051‑M2/46‑116 (MQ=255) CGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTc < 3:43547‑M1/80‑1 (MQ=255) CGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTc > 4:43547‑M1/1‑80 (MQ=255) GCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACaa > 8:54178‑M1/1‑120 (MQ=255) GCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACaa < 7:54178‑M1/120‑1 (MQ=255) gcggaaaccctgagtgggattgcgctgtcagggcgGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCAGAATCCATGc < 8:31143‑M2/114‑1 (MQ=255) gcAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACgg < 5:4859/115‑1 (MQ=255) gcAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACgg > 6:4859/1‑115 (MQ=255) aTCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGTGc < 2:150722/149‑1 (MQ=255) aCTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGTGCCTCtt < 5:70350/149‑1 (MQ=255) cGCTTCAAGTCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACTCATTTCCATGCCGATACGGGATCGACAATTACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGTGCCTCTTc < 3:66082/138‑1 (MQ=255) gCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCa < 1:119035/115‑1 (MQ=255) gCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCa > 2:119035/1‑115 (MQ=255) gTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTAc < 6:93301/87‑1 (MQ=255) gTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTAc > 5:93301/1‑87 (MQ=255) | ACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGGTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGTGCCTCTTC > NC_000913/2258589‑2258861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |