| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 123,061 | C→T | R15R (CGC→CGT) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 123,061 | 0 | C | T | 100.0% | 15.3 / NA | 6 | R15R (CGC→CGT) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (3/3); total (3/3) | |||||||||||
GGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATC > NC_000913/122968‑123079 | ggACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGACTGGCTCCAGGCg < 3:63323/109‑1 (MQ=255)ggACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGACTGGCTCCAGGCg > 4:63323/1‑109 (MQ=255) tttCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGACTGGCTCCAGGCggtg < 6:4824/105‑4 (MQ=255) gTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGACTGGCTCCAGGCggtg > 5:4824/1‑103 (MQ=255) aTAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGa > 7:69669/1‑54 (MQ=255) aTAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGa < 8:69669/54‑1 (MQ=255) | GGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATC > NC_000913/122968‑123079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |