Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 911,086 IS2 (–) +5 bp intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)16 (0.630) 16/256 0.6 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 911086 = 0 (0.000)intergenic (‑37/+96) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
* ? NC_000913 = 382590NA (NA)16 (0.640) 16/254 0.6 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 911090 0 (0.000)intergenic (‑41/+92) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

AGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCC  >  NC_000913/910943‑911224
                                                                                                                                                   |                                                                                                                                      
aGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAg                                                                                                                                        <  3:41535/148‑1 (MQ=255)
       cACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGt                                                                                                                                       >  3:33705‑M2/1‑141 (MQ=255)
       cACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGt                                                                                                                                       <  4:33705‑M2/142‑2 (MQ=255)
            aGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccc                                                                                                                            <  3:102682‑M2/148‑13 (MQ=255)
                     agagagTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttcca                                                                                                                  >  4:102682‑M2/1‑127 (MQ=255)
                           tctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATgg                                                                                                             <  7:74924‑M2/32‑1 (MQ=255)
                             tccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATgg                                                                                                             >  5:5099‑M2/115‑146 (MQ=255)
                                  tgACTCCCCAGCTGCGTTTCACCCCGGCCGATTCGAGTGTTTTGGCGAAATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCACCTGAATGTGATAACGGTAACAAGTTTAGtggctttgcccctatatttcaagacgactggt                                                                                                        <  8:65192‑M2/146‑33 (MQ=255)
                                       atacatcaatcatctgttctacaatgtatagtctaaaaacttcttttaatcctatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAaggagg                                                                                                <  6:5099‑M2/45‑1 (MQ=255)
                                                        cccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttat                                                                                                      <  6:101436‑M2/126‑35 (MQ=255)
                                                        cccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttat                                                                                                      >  5:101436‑M2/1‑92 (MQ=255)
                                                            caatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCAt                                                                                                                                   <  6:5922‑M2/10‑1 (MQ=255)
                                                            caatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCAt                                                                                                                                   >  5:5922‑M2/84‑93 (MQ=255)
                                                               gATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaaccc                                                                                            <  6:66960‑M2/129‑45 (MQ=255)
                                                               gATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaaccc                                                                                            >  5:66960‑M2/1‑85 (MQ=255)
                                                                   tagtctaaaaactagtattaagactatcacttatttaagtgctattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGTGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAg                                                                    >  7:35057‑M2/77‑149 (MQ=255)
                                                                         ttttGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccg                                                              <  2:11356‑M2/149‑75 (MQ=255)
                                                                          aaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAatat                                                              >  3:104516‑M2/70‑148 (MQ=255)
                                                                             ggCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtgg                                                           >  2:100315‑M2/1‑71 (MQ=255)
                                                                              gCGATCTAAGCTGCAACCGTTTGGTTCATGGTTCTCCATGTCCTGTATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgcagccgcagatattcccgtggc                                                          >  7:65192‑M2/1‑70 (MQ=255)
                                                                               cGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcga                                                        >  1:11356‑M2/1‑69 (MQ=255)
                                                                                 gtattaagactatcacttatttcagtgaaattggctgcctgccgatgcagggggccagcctaTTTAGCCCATCTGCCGGAGGGGCAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCgg                                                        <  4:104516‑M2/85‑1 (MQ=255)
                                                                                                               ttgggtgtcgggagatccaggtggccagcctaTTTCGTTCCTCGGACGGAGGGTGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATccc                         <  8:35057‑M2/116‑1 (MQ=255)
                                                                                                                  cATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcg                      >  5:21321‑M2/1‑34 (MQ=255)
                                                                                                                       ctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGAt                >  6:120995‑M2/25‑149 (MQ=255)
                                                                                                                       ccTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgag                                                       >  1:28168‑M2/1‑29 (MQ=255)
                                                                                                                       ccTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgag                                                       <  2:28168‑M2/110‑82 (MQ=255)
                                                                                                                                       ccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGcc  >  6:28138‑M2/9‑147 (MQ=255)
                                                                                                                                       ccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGcc  <  5:28138‑M2/139‑1 (MQ=255)
                                                                                                                                            aaGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgc                            >  4:36679‑M2/1‑8 (MQ=255)
                                                                                                                                            aaGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgc                            <  3:36679‑M2/116‑109 (MQ=255)
                                                                                                                                              aTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgaga                                   >  7:59693‑M2/2‑107 (MQ=255)
                                                                                                                                              aTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgaga                                   <  8:59693‑M2/106‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                      
AGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCC  >  NC_000913/910943‑911224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.