Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,413,461	G→A	intergenic (‑329/‑9)	yfbV ← / → ackA	UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,413,461	0	G	A	100.0%	117.3 / NA	34	intergenic (‑329/‑9)	yfbV/ackA	UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/16);  total (18/16)

TACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCAC  >  NC_000913/2413318‑2413596                                                                                                                                                |                                                                                                                                        tACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACt                                                                                                                                    >  6:49876/1‑149 (MQ=255)    gCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                 <  5:49876/149‑1 (MQ=255)                   cTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTAGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACTTATCAATTATAAGTACTTCCATCTAGAGTAAGt                                                                                                                    >  7:27636/1‑147 (MQ=255)                    tCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAg                                                                                                                 >  4:82983/1‑149 (MQ=255)                                    atcatGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGCACTg                                                                                                 >  7:82728/1‑149 (MQ=255)                                       atGCAAAAAAAATTTGCGGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCgg                                                                                              >  5:8718/1‑149 (MQ=255)                                             aaaaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                 <  8:30769/108‑1 (MQ=255)                                             aaaaaaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTcc                                                                                                                                 >  7:30769/1‑108 (MQ=255)                                                   tttGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTc                                                                                                        <  3:73354/127‑1 (MQ=255)                                                   tttGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTc                                                                                                        >  4:73354/1‑127 (MQ=255)                                                            cATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGc                                                                         >  6:72614/1‑149 (MQ=255)                                                             atgatgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGcc                                                                        <  6:8718/149‑1 (MQ=255)                                                               gatgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTc                                                                                                        >  3:74437/1‑115 (MQ=255)                                                               gatgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTc                                                                                                        <  4:74437/115‑1 (MQ=255)                                                                  gTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGaa                                                                                                    >  4:49438/1‑116 (MQ=255)                                                                   ttAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcatcat                                                                   <  3:82983/148‑1 (MQ=255)                                                                    tAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGt                                                                                                                    >  5:16580/1‑98 (MQ=255)                                                                      aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAATTTAGTACTGGTTc                                                                                                        >  8:83808/1‑108 (MQ=255)                                                                      aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTc                                                                                                        <  7:83808/108‑1 (MQ=255)                                                                      aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGAt                                                               <  5:72614/149‑1 (MQ=255)                                                                          tAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAg                                                           >  1:3785/1‑149 (MQ=255)                                                                                tCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATg                                                     <  8:82728/149‑1 (MQ=255)                                                                                       catcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTg                                                                                 >  4:55864/1‑114 (MQ=255)                                                                                       catcatGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTg                                                                                 <  3:55864/114‑1 (MQ=255)                                                                                                 tACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAttt                                                                           <  2:35444/110‑1 (MQ=255)                                                                                                 tACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAttt                                                                           >  1:35444/1‑110 (MQ=255)                                                                                                                    aCGTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGa                                                 <  4:81522/117‑1 (MQ=255)                                                                                                                      gTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCt                                        >  4:101843/1‑124 (MQ=255)                                                                                                                      gTTTTTTTAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCt                                        <  3:101843/124‑1 (MQ=255)                                                                                                                        ttttttAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTaaa                                                       >  5:67928/1‑107 (MQ=255)                                                                                                                        ttttttAGCCACGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTaaa                                                       <  6:67928/107‑1 (MQ=255)                                                                                                                                   cGTATCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCAc  <  2:3785/149‑1 (MQ=255)                                                                                                                                      aTCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGt                                                          <  4:14172/90‑1 (MQ=255)                                                                                                                                      aTCAATTATAAGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGt                                                          >  3:14172/1‑90 (MQ=255)                                                                                                                                                |                                                                                                                                        TACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCAC  >  NC_000913/2413318‑2413596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑