Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,657,4340GT20.7% 69.0 / 11.9 29S501Y (TCT→TAT) ‡hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base G (15/8);  new base T (3/3);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGC  >  NC_000913/2657296‑2657582
                                                                                                                                          |                                                                                                                                                    
agcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGAc                                                                                                                                            >  4:62299/1‑149 (MQ=255)
     gcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGaa                                                                                                                                       >  8:79827/1‑149 (MQ=255)
         tGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGa                                                                                                                                   <  7:79827/149‑1 (MQ=255)
                tttCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTc                                                                                                                             >  6:52347/1‑148 (MQ=255)
                  tCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCaa                                                                                                                           <  5:52347/148‑1 (MQ=255)
                                              gTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAAt                                                                                                                                      <  1:74247/109‑1 (MQ=255)
                                              gTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAAt                                                                                                                                      >  2:74247/1‑109 (MQ=255)
                                                    ccAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGAc                                                                                                                                            <  3:85232/97‑1 (MQ=255)
                                                    ccAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGAc                                                                                                                                            >  4:85232/1‑97 (MQ=255)
                                                        cATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGa                                                                                                                                             <  3:32407/92‑1 (MQ=255)
                                                        cATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGa                                                                                                                                             >  4:32407/1‑92 (MQ=255)
                                                                          cTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGAc                                                                       <  8:23064/144‑1 (MQ=255)
                                                                          cTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGAc                                                                       >  7:23064/1‑144 (MQ=255)
                                                                            gCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAAc                                                                >  7:35291/1‑149 (MQ=255)
                                                                                  cATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTc                                                                                <  5:110161/127‑1 (MQ=255)
                                                                                  cATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTc                                                                                >  6:110161/1‑127 (MQ=255)
                                                                                                tCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTc                                                             >  5:97145/1‑132 (MQ=255)
                                                                                                tCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTc                                                             <  6:97145/132‑1 (MQ=255)
                                                                                                tCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATgcgctac                                            >  1:141448/1‑146 (MQ=255)
                                                                                                  tttGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGc                                          >  2:93719/1‑149 (MQ=255)
                                                                                                         atcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCgg                                   >  6:119023/1‑149 (MQ=255)
                                                                                                         atcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCgg                                   >  6:121040/1‑149 (MQ=255)
                                                                                                                     tCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGaa                        <  8:35291/148‑1 (MQ=255)
                                                                                                                       gCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATAc                     >  6:44845/1‑149 (MQ=255)
                                                                                                                           caggTCAAACCGTCTTACGGTCTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCgg                                   >  4:129039/3‑131 (MQ=255)
                                                                                                                           caggTCAAACCGTCTTACGGTCTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCgg                                   <  3:129039/129‑1 (MQ=255)
                                                                                                                                  gACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGc                                                                                             >  2:137779/1‑66 (MQ=255)
                                                                                                                                  gACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGc                                                                                             <  1:137779/66‑1 (MQ=255)
                                                                                                                                     cGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCaaaa       >  1:117940/1‑149 (MQ=255)
                                                                                                                                          gACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACgcgc  <  3:62299/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
AGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGC  >  NC_000913/2657296‑2657582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑